2,4-Dichlorophenoxy acetic acid, abscisic acid, and hydrogen peroxide induced resistance-related components against potato early blight (Alternaria solani, Sorauer)

Nassar, Atef; Adss, Ibrahim A.

doi:10.1016/j.aoas.2016.04.005

Cited by 14 publications

(5 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, the use of PAL inhibitors AIP and AOPP (100 µM) inhibited lignin accumulation in tracheary elements (Dennis et al 2004 ). PAL is the key enzyme for lignification barriers to fungal invasion into the cell walls of VBs (Cass et al 2015 ) and was shown to be regulated by the herbicide 2,4-DPA in potato plants (Nassar and Adss 2016 ). Treatment of seedlings with AOPP (Pan et al 2008 ), 1.0 mM 2,4-DPA, and 0.5 M acetic acid (Tomás-Barberán et al 1997 ), a chemical inhibitor of PAL activity, reduced the accumulation of lignin content.…”

Section: Discussionmentioning

confidence: 99%

Immunocompromisation of wheat host by L-BSO and 2,4-DPA induces susceptibility to the fungal pathogen Fusarium oxysporum

Sahu,

Kumari,

Mittra

2024

Stress Biology

View full text Add to dashboard Cite

Susceptibility is defined as the disruption of host defence systems that promotes infection or limits pathogenicity. Glutathione (GSH) is a major component of defence signalling pathways that maintain redox status and is synthesised by γ-glutamyl cysteine synthetase (γ-ECS). On the other hand, lignin acts as a barrier in the primary cell wall of vascular bundles (VBs) synthesised by phenylalanine ammonia-lyase (PAL) in the intracellular system of plants. In this study, we used two inhibitors, such as L-Buthionine-sulfoximine (BSO), which irreversibly inhibits γ-ECS, and 2,4-dichlorophenoxyacetic acid (DPA), which reduces PAL activity and leads to the induction of oxidative stress in wheat (Triticum aestivum) seedlings after exposure to Fusarium oxysporum. Seedlings treated with 1 mM L-BSO and 2,4-DPA showed high levels of hydrogen peroxide (H2O2), malondialdehyde (MDA), carbonyl (CO) content, and low activity of antioxidative enzymes [superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR)] as compared to wild-type (WT) seedlings under F. oxysporum infection. Further, the content of reduced glutathione (RGSH), ascorbate (ASC), and lignin was decreased in BSO and DPA treated seedlings as compared to WT seedlings during Fusarium infection. Moreover, treatment with BSO and DPA significantly inhibited the relative activity of γ-ECS and PAL (P ≤ 0.001) in WT seedlings during Fusarium infection, which led to disintegrated VBs and, finally, cell death. Our results demonstrate that inhibition of γ-ECS and PAL by BSO and DPA, respectively, disrupts the defence mechanisms of wheat seedlings and induces susceptibility to F. oxysporum.

show abstract

Section: Discussionmentioning

confidence: 99%

Immunocompromisation of wheat host by L-BSO and 2,4-DPA induces susceptibility to the fungal pathogen Fusarium oxysporum

Sahu,

Kumari,

Mittra

2024

Stress Biology

View full text Add to dashboard Cite

show abstract

“…Though we did not examine the ethylene content, the significantly increased levels of precursors for ethylene biosynthesis in the stylar end and the intermediate segment of cucumber fruit in 2, 4-D groups at 4 DAF ( Figure 4 ) might be an indicator of ethylene accumulation in 2,4-D treated cucumber fruit. Excessive use of 2,4-D often results in the generation of reactive oxygen species (ROS) that is associated with enhanced antioxidant pathways as illustrated in 2,4-D treated mung beans and peas [ 44 , 45 ], mango fruit under chilling injury [ 48 ] and potato infected with Alternaria solani [ 49 ]. The observed significant increased levels of some oxidation products, such as oxidized lipids, and contrastingly decreased levels of antioxidants, such as ascorbate, in the stylar end of the cucumber fruit at 10 DAF, indicated that 2,4-D treatment resulted in the generation of ROS in cucumber fruit.…”

Section: Discussionmentioning

confidence: 99%

Effects of 2,4-Dichlorophenoxyacetic Acid on Cucumber Fruit Development and Metabolism

Zhao

Shi

et al. 2019

IJMS

View full text Add to dashboard Cite

The auxin-like compound 2,4-dichlorophenoxyacetic acid (2,4-D) has been widely used as a plant growth regulator in cucumber fruit production; however, its influence on fruit development and metabolism has not been evaluated. In this study, the phenotype of cucumber fruits in both 2,4-D treatment and non-treatment control groups were recorded, and the metabolome of different segments of cucumber fruit at various sampling time points were profiled by a standardized non-targeted metabolomics method based on UPLC-qTOF-MS. The application of 2,4-D increased the early growth rate of the fruit length but had no significant effect on the final fruit length, and produced cucumber fruits with fresh flowers at the top. The 2,4-D treatment also affected the cucumber fruit metabolome, causing significant changes in the stylar end at 4 days after flowering (DAF). The significantly changed metabolites were mainly involved in methionine metabolism, the citric acid cycle and flavonoid metabolism pathways. At the harvest stage, 2,4–D treatment significantly decreased the levels of flavonoids and cinnamic acid derivatives while increased the levels of some of the amino acids. In summary, exogenous application of 2,4-D can greatly alter the phenotype and metabolism of cucumber fruit. These findings will assist in exploring the mechanisms of how 2,4-D treatment changes the fruit phenotype and evaluating the influence of 2,4-D treatment on the nutritional qualities of cucumber fruit.

show abstract

“…To obtain a dataset with sufficient variation, and hence construct a robust model, different cultivars were used during different years. The choice was made by a plant pathologist, focusing on varieties with medium to high susceptibility to Alternaria solani , namely Spunta Nassar and Adss (2016) , Bintje Van De Vijver et al. (2020) and Fontane Duarte et al.…”

Section: Methodsmentioning

confidence: 99%

Ultra-high-resolution UAV-imaging and supervised deep learning for accurate detection of Alternaria solani in potato fields

Wieme,

Leroux,

Cool

et al. 2024

Front. Plant Sci.

View full text Add to dashboard Cite

Alternaria solani is the second most devastating foliar pathogen of potato crops worldwide, causing premature defoliation of the plants. This disease is currently prevented through the regular application of detrimental crop protection products and is guided by early warnings based on weather predictions and visual observations by farmers. To reduce the use of crop protection products, without additional production losses, it would be beneficial to be able to automatically detect Alternaria solani in potato fields. In recent years, the potential of deep learning in precision agriculture is receiving increasing research attention. Convolutional Neural Networks (CNNs) are currently the state of the art, but also come with challenges, especially regarding in-field robustness. This stems from the fact that they are often trained on datasets that are limited in size or have been recorded in controlled environments, not necessarily representative of real-world settings. We collected a dataset consisting of ultra-high-resolution modified RGB UAV-imagery of both symptomatic and non-symptomatic potato crops in the field during various years and disease stages to cover the great variability in agricultural data. We developed a convolutional neural network to perform in-field detection of Alternaria, defined as a binary classification problem. Our model achieves a similar accuracy as several state-of-the-art models for disease detection, but has a much lower inference time, which enhances its practical applicability. By using training data of three consecutive growing seasons (2019, 2020 and 2021) and test data of an independent fourth year (2022), an F1 score of 0.93 is achieved. Furthermore, we evaluate how different properties of the dataset such as its size and class imbalance impact the obtained accuracy.

show abstract

2,4-Dichlorophenoxy acetic acid, abscisic acid, and hydrogen peroxide induced resistance-related components against potato early blight (Alternaria solani, Sorauer)

Cited by 14 publications

References 30 publications

Immunocompromisation of wheat host by L-BSO and 2,4-DPA induces susceptibility to the fungal pathogen Fusarium oxysporum

Immunocompromisation of wheat host by L-BSO and 2,4-DPA induces susceptibility to the fungal pathogen Fusarium oxysporum

Effects of 2,4-Dichlorophenoxyacetic Acid on Cucumber Fruit Development and Metabolism

Ultra-high-resolution UAV-imaging and supervised deep learning for accurate detection of Alternaria solani in potato fields

Contact Info

Product

Resources

About