2,5‐Dihydroxybenzoic acid salts for matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometric lipid analysis: Simplified spectra interpretation and insights into gas‐phase fragmentation

Abstract

Neutral DHB salts avoid separation of lipid species into several ionization states when used as matrices in MALDI-TOF MS. This allows for simplified lipid spectra interpretation. Due to the high cationization efficiency of DHB matrix salts, certain lipid classes become detectable which cannot be analyzed easily using standard acidic DHB.

Simple approach to derivatization of alcohols and phenols for the analysis by matrix(surface)‐assisted laser desorption/ionization time‐of‐flight mass spectrometry

“…Some recent papers describe new derivatization techniques for use with GC/MS, ESI‐MS, and MALDI‐TOFMS in the analysis of steroid alcohols. Application of non‐derivatization MALDI‐MS with DHB salts as matrices to sterol analysis has been tested as well …”

“…Application of non-derivatization MALDI-MS with DHB salts as matrices to sterol analysis has been tested as well. [26] Figures 4(a)-4(c) show the MALDI mass spectra of a mixture of six sterols derivatized by bromoacetyl chloride in the presence of triethylamine, pyridine or D 5 -pyridine. The peaks in the spectrum correspond to masses of the cationic parts of these six sterol derivatives, no other peaks being observed.…”

Direct Analysis of Human Sputum for Differentiating Non-small Cell Lung Cancer by Neutral Desorption Extractive Electrospray Ionization Mass Spectrometry

“…Therefore, the signal detected at m/z 756 was attributed to dipalmitoyl phosphatidylcholine sodium salt, a main component of pulmonary surfactant. [29][30][31]…”

Addition of CsCl reduces ion suppression effects in the matrix‐assisted laser desorption/ionization mass spectra of triacylglycerol/phosphatidylcholine mixtures and adipose tissue extracts

“…The mass spectrometer was calibrated using a lipid mixture of known composition. As DHB is a suitable matrix for both, 337 and 355 nm lasers, experiments can be also performed by using Nd:YAG lasers …”