2‐Aminoethylphosphonate utilization in <i>Pseudomonas putida</i> <scp>BIRD</scp>‐1 is controlled by multiple master regulators

Murphy, Andrew; Scanlan, David J.; Chen, Yin; Bending, Gary D.; Hammond, John P.; Wellington, E. M. H.; Lidbury, Ian

doi:10.1111/1462-2920.15959

Cited by 4 publications

(7 citation statements)

References 69 publications

(136 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Likewise, in marine systems, phnJ and phnD gene abundances and expression levels correlate negatively with Pi concentrations [ 62 , 66 ]. However, in recent transcriptional studies, Teikari et al [ 11 ] and Murphy et al [ 70 ] suggested that the expression of phnG-M and other phosphonate genes might be activated only in the presence of a suitable phosphonate substrate, based on the bipartite structure of the C-P lyase gene cluster. That proposal is supported by results seen in our coculture systems (Fig.…”

Section: Discussionmentioning

confidence: 99%

The facilitating role of phycospheric heterotrophic bacteria in cyanobacterial phosphonate availability and Microcystis bloom maintenance

Zhao,

Lin,

Zeng

et al. 2023

Microbiome

View full text Add to dashboard Cite

Background Phosphonates are the main components in the global phosphorus redox cycle. Little is known about phosphonate metabolism in freshwater ecosystems, although rapid consumption of phosphonates has been observed frequently. Cyanobacteria are often the dominant primary producers in freshwaters; yet, only a few strains of cyanobacteria encode phosphonate-degrading (C-P lyase) gene clusters. The phycosphere is defined as the microenvironment in which extensive phytoplankton and heterotrophic bacteria interactions occur. It has been demonstrated that phytoplankton may recruit phycospheric bacteria based on their own needs. Therefore, the establishment of a phycospheric community rich in phosphonate-degrading-bacteria likely facilitates cyanobacterial proliferation, especially in waters with scarce phosphorus. We characterized the distribution of heterotrophic phosphonate-degrading bacteria in field Microcystis bloom samples and in laboratory cyanobacteria “phycospheres” by qPCR and metagenomic analyses. The role of phosphonate-degrading phycospheric bacteria in cyanobacterial proliferation was determined through coculturing of heterotrophic bacteria with an axenic Microcystis aeruginosa strain and by metatranscriptomic analysis using field Microcystis aggregate samples. Results Abundant bacteria that carry C-P lyase clusters were identified in plankton samples from freshwater Lakes Dianchi and Taihu during Microcystis bloom periods. Metagenomic analysis of 162 non-axenic laboratory strains of cyanobacteria (consortia cultures containing heterotrophic bacteria) showed that 20% (128/647) of high-quality bins from eighty of these consortia encode intact C-P lyase clusters, with an abundance ranging up to nearly 13%. Phycospheric bacterial phosphonate catabolism genes were expressed continually across bloom seasons, as demonstrated through metatranscriptomic analysis using sixteen field Microcystis aggregate samples. Coculturing experiments revealed that although Microcystis cultures did not catabolize methylphosphonate when axenic, they demonstrated sustained growth when cocultured with phosphonate-utilizing phycospheric bacteria in medium containing methylphosphonate as the sole source of phosphorus. Conclusions The recruitment of heterotrophic phosphonate-degrading phycospheric bacteria by cyanobacteria is a hedge against phosphorus scarcity by facilitating phosphonate availability. Cyanobacterial consortia are likely primary contributors to aquatic phosphonate mineralization, thereby facilitating sustained cyanobacterial growth, and even bloom maintenance, in phosphate-deficient waters.

show abstract

Section: Discussionmentioning

confidence: 99%

The facilitating role of phycospheric heterotrophic bacteria in cyanobacterial phosphonate availability and Microcystis bloom maintenance

Zhao,

Lin,

Zeng

et al. 2023

Microbiome

View full text Add to dashboard Cite

show abstract

“…As in the case of other metabolic networks, the expression of bacterial genes involved in phosphonate catabolism is under tight control. Such control can vary substantially depending on the organism and on the pathway and can be complex and multi-layered [126,127]. Below, we try to provide some general principles on the phenomenon, while illustrating some well-studied examples of regulation.…”

Section: Regulation Of Phosphonate Degradation Pathwaysmentioning

confidence: 99%

“…Further studies revealed that a number of other Alphaproteobacteria possessing phnWX and phnWYA operons were also able to grow on AEP as the sole N source in the presence of phosphate [39]. In a recent, detailed study [126], Murphy et al provided evidence that the AEP catabolism in Pseudomonas putida BIRD1 is not only under the PhoBR regulation in phosphate-limiting conditions. Specifically, phnWX gene expression also occurred under C or N depletion, and it was regulated by the two-component system regulators CbrAB and NtrBC, respectively [126].…”

Section: Substrate-specific Hydrolytic Pathways Expression Can Be Pho...mentioning

confidence: 99%

“…In a recent, detailed study [126], Murphy et al provided evidence that the AEP catabolism in Pseudomonas putida BIRD1 is not only under the PhoBR regulation in phosphate-limiting conditions. Specifically, phnWX gene expression also occurred under C or N depletion, and it was regulated by the two-component system regulators CbrAB and NtrBC, respectively [126].…”

Section: Substrate-specific Hydrolytic Pathways Expression Can Be Pho...mentioning

confidence: 99%

See 1 more Smart Citation

The Microbial Degradation of Natural and Anthropogenic Phosphonates

Ruffolo,

Dinhof,

Murray

et al. 2023

Molecules

View full text Add to dashboard Cite

Phosphonates are compounds containing a direct carbon–phosphorus (C–P) bond, which is particularly resistant to chemical and enzymatic degradation. They are environmentally ubiquitous: some of them are produced by microorganisms and invertebrates, whereas others derive from anthropogenic activities. Because of their chemical stability and potential toxicity, man-made phosphonates pose pollution problems, and many studies have tried to identify biocompatible systems for their elimination. On the other hand, phosphonates are a resource for microorganisms living in environments where the availability of phosphate is limited; thus, bacteria in particular have evolved systems to uptake and catabolize phosphonates. Such systems can be either selective for a narrow subset of compounds or show a broader specificity. The role, distribution, and evolution of microbial genes and enzymes dedicated to phosphonate degradation, as well as their regulation, have been the subjects of substantial studies. At least three enzyme systems have been identified so far, schematically distinguished based on the mechanism by which the C–P bond is ultimately cleaved—i.e., through either a hydrolytic, radical, or oxidative reaction. This review summarizes our current understanding of the molecular systems and pathways that serve to catabolize phosphonates, as well as the regulatory mechanisms that govern their activity.

show abstract

“…The “omics” techniques (genomics, transcriptomics, proteomics or metabolomics) provide holistic views that increase the global knowledge of the processes under study, such as the identification of metabolic responses and molecular mechanisms to nutrient stress ( Lidbury et al, 2016 , 2021 ; Murphy et al, 2022 ) or conditions like diazotrophic vs. non-diazotrophic conditions ( Madeira et al, 2020 ; Nkongolo and Narendrula-Kotha, 2020 ). Since proteins are the final molecular effectors of gene functions, proteomics emerged as a necessary tool for the identification of proteins in a biological system, defining the proteome as the set of proteins expressed in a given organism/cell in response to certain conditions ( Anderson and Anderson, 1998 ).…”

Section: Introductionmentioning

confidence: 99%

Holistic view of biological nitrogen fixation and phosphorus mobilization in Azotobacter chroococcum NCIMB 8003

Biełło

Lucena²,

López-Tenllado³

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

Nitrogen (N) and phosphorus (P) deficiencies are two of the most agronomic problems that cause significant decrease in crop yield and quality. N and P chemical fertilizers are widely used in current agriculture, causing environmental problems and increasing production costs. Therefore, the development of alternative strategies to reduce the use of chemical fertilizers while maintaining N and P inputs are being investigated. Although dinitrogen is an abundant gas in the atmosphere, it requires biological nitrogen fixation (BNF) to be transformed into ammonium, a nitrogen source assimilable by living organisms. This process is bioenergetically expensive and, therefore, highly regulated. Factors like availability of other essential elements, as phosphorus, strongly influence BNF. However, the molecular mechanisms of these interactions are unclear. In this work, a physiological characterization of BNF and phosphorus mobilization (PM) from an insoluble form (Ca3(PO4)2) in Azotobacter chroococcum NCIMB 8003 was carried out. These processes were analyzed by quantitative proteomics in order to detect their molecular requirements and interactions. BNF led to a metabolic change beyond the proteins strictly necessary to carry out the process, including the metabolism related to other elements, like phosphorus. Also, changes in cell mobility, heme group synthesis and oxidative stress responses were observed. This study also revealed two phosphatases that seem to have the main role in PM, an exopolyphosphatase and a non-specific alkaline phosphatase PhoX. When both BNF and PM processes take place simultaneously, the synthesis of nitrogenous bases and L-methionine were also affected. Thus, although the interdependence is still unknown, possible biotechnological applications of these processes should take into account the indicated factors.

show abstract

2‐Aminoethylphosphonate utilization in Pseudomonas putida BIRD‐1 is controlled by multiple master regulators

Cited by 4 publications

References 69 publications

The facilitating role of phycospheric heterotrophic bacteria in cyanobacterial phosphonate availability and Microcystis bloom maintenance

The facilitating role of phycospheric heterotrophic bacteria in cyanobacterial phosphonate availability and Microcystis bloom maintenance

The Microbial Degradation of Natural and Anthropogenic Phosphonates

Holistic view of biological nitrogen fixation and phosphorus mobilization in Azotobacter chroococcum NCIMB 8003

Contact Info

Product

Resources

About