2-Aminopyridine as a standard for low-wavelength spectrofluorimetry

Absolute spectrofluorometry

“…13). The corrected fluo· rescence spectrum of 2-amino pyridine de termined by Rusakowicz and T esta [45] usin g a tun gste n lamp for calibration is given in table 5. Th e spectrum meas ured b L = tungsten lamp calibration, Q = quantum counter calibration in this laboratory using the quantum counter met,hod of calibration is in good agreement with their spectrum.…”

“…A number of absolute standards have been proposed, notably by Lippert et al [5], but the quinine spectrum is the only one measured by a number of authors. The new standard 2-amino pyridine proposed by Rusakowicz and Testa [45] covers approximately the same wavelength range as 2-naphthol and is to be preferred since it does not have the temperature effects shown by the latter.…”

Reference materials for fluorescence measurement

“…Melhuish [12] also presented a corrected spectrum for 2-aminopyridine in 1 N H2SO4 (excitation 280-330 nm, emission 340-450 nm). This standard is recommended in that wavelength region by Testa [14]. PCarboline has also been proposed as a standard for the 450 nm range [15].…”

DOC, absorption and fluorescence properties of estuarine and coastal waters of the Northern Tyrrhenian Sea

“…2-amino-pyridine was used as standard for the fluorescence excited at 280 nm and quinine sulfate for that excited at 355 nm. Literature ⌽ o (r) were 0.6 for 2-amino-pyridine (Rusakowicz and Testa, 1968) and 0.51 for quinine sulfate (Green and Blough, 1994).…”