2-arylpropionyl-CoA epimerase: Partial peptide sequences and tissue localization

Renal d-Amino Acid Oxidase Mediates Chiral Inversion ofNG-Nitro-d-arginine

“…Chiral inversion is normally unidirectional in various species including humans, e.g., from R to S inversion for profens, whereas S to R inversion is rare (Jamali et al, 1997). The long-chain acyl-CoA synthetase and 2-arylpropionyl-CoA epimerase have been identified as key enzymes involved in the chiral inversion of 2-arylpropionic acids (Reichel et al, 1995(Reichel et al, , 1997Brugger et al, 1996). Epimerase mRNA and protein are strongly expressed in the rat liver and kidneys and weakly expressed in heart and brain (Reichel et al, 1995(Reichel et al, , 1997.…”

“…The long-chain acyl-CoA synthetase and 2-arylpropionyl-CoA epimerase have been identified as key enzymes involved in the chiral inversion of 2-arylpropionic acids (Reichel et al, 1995(Reichel et al, , 1997Brugger et al, 1996). Epimerase mRNA and protein are strongly expressed in the rat liver and kidneys and weakly expressed in heart and brain (Reichel et al, 1995(Reichel et al, , 1997. Thus, the chiral inversion process for D-amino acids to L-enantiomers is distinctly different from that for 2-arylpropionic acids.…”

Molecular Cloning and Expression of a 2-Arylpropionyl-Coenzyme A Epimerase: A Key Enzyme in the Inversion Metabolism of Ibuprofen

“…An important pathway in the metabolism of 2-arylpropionic acids is the chiral inversion in which the (Ϫ)-R-enantiomers, which are inactive in terms of COX inhibition, are inverted to the COXinhibiting (ϩ)-S form (2,3). Chiral inversion has been investigated in cellular homogenates (4,5), whole isolated cells (6,7), and whole perfused organs (8) including lung (9) and kidney (10). The mechanism of chiral inversion involves three metabolic steps (Fig.…”

“…In the following reaction (Fig. 1B), the resulting (Ϫ)-(R)-ibuprofenoyl-CoA thioester is epimerized (14,15) via a cytosolic and mitochondrial enzyme (16,5), the 2-arylpropionyl-CoA epimerase. Hydrolysis of the thioesters (Fig.…”

“…We raised polyclonal antibodies against the epimerase and analyzed the relationship between the inversion activity in various tissues and the tissue distribution of the protein in guinea pigs and rats (5). Furthermore, three internal peptides of the epimerase were identified (5). To facilitate research into the physiological and pharmacological function of the enzyme, it was necessary to identify the gene structure of the 2-arylpropio-nyl-CoA epimerase.…”