2-D Clinostat for Simulated Microgravity Experiments with Arabidopsis Seedlings

Wang, Hui; Li, Xugang; Krause, Lars; Görög, Mark; Schüler, Oliver; Hauslage, Jens; Hemmersbach, Ruth; Kircher, Stefan; Lasok, Hanna; Haser, Thomas; Rapp, Katja; Schmidt, Jürgen; Yu, Xin; Pasternak, Taras; Aubry-Hivet, Dorothée; Tietz, Olaf; Dovzhenko, Alexander; Palme, Klaus; Ditengou, Franck Anicet

doi:10.1007/s12217-015-9478-1

Cited by 20 publications

(8 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Application of clinorotation, where plant is not gravistimulated, but disorientated in relation to gravity vector, is a good contribution to the research in this field (Kordyum, ; Wang et al, ). Clinorotated plants do not sense direct impact of gravity vector and this could possibly, unmask mechanisms of graviperception hidden under constant gravity.…”

Section: Introductionmentioning

confidence: 99%

Putative gravisensors among microtubule associated proteins

Shevchenko¹

2017

Cell Biology International

View full text Add to dashboard Cite

Despite of long period of investigation (over 100 years), still a lot of questions remain unclear about molecular mechanisms of plant graviperception. This requires designing new experiments and new approaches to be applied in gravitational biology. Investigation of plant cell reactions under clinorotation (plant disorientation in respect to gravity vector) is of significant importance to such type of research. Clinorotation is known to cause changes of cell polarity and exert mechanical stress in plant cells. Microtubular cytoskeleton is highly dynamic structure and it responds to both of these stresses. Due to turgor pressure and cell elongation, endogenous mechanical forces influence microtubule orientation in order to coordinate cell growth. Rearrangements of microtubules are regulated by numerous associated proteins which functional activity is not fully clear. In this review, we discuss how MT associated proteins regulate cortical MT arrays under mechanical stress and consider how these proteins may act as plant cell gravisensors. Investigation of microtubule associated proteins under clinorotation might shed the light on molecular mechanism of plant cytoskeleton arrangement and its involvement in initial reactions of cell graviperception.

show abstract

Section: Introductionmentioning

confidence: 99%

Putative gravisensors among microtubule associated proteins

Shevchenko¹

2017

Cell Biology International

View full text Add to dashboard Cite

show abstract

“…Thus, fast clinorotation would only be appropriate in a very limited radius from the center of rotation, or for short times of microgravity simulation. Previously, researchers applied the criterion of centrifugal force limit to determine the usable space for effective microgravity simulation during clinorotation [ 31 , 38 , 73 , 74 ]. These limits ranged between 0.00009 g – 0.2 g and were determined by investigating the minimal centrifugal force that caused directional growth in oats clinorotated horizontally (0.0001 g) [ 41 ], in lentils in a centrifuge in the GRAVI-1 space experiment (0.000014 g) [ 42 ], or the partial- g effect on rhizoids of Chara globularis in parabolic flights (0.05 g) [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

“…Despite the common use of clinostats and the vast research that was performed on these devices, no clear set of rules has been established as for the optimal clinostat settings. Angular velocity varies between studies from 1–2 rpm up to 60 rpm without a justification [ 31 , 32 , 33 , 34 , 35 ]. In addition, we can distinguish two types of clinorotation depending on the orientation of the sample in relation to the clinorotation axis: vertical clinorotation (VC), with the longitudinal growth axis of the plant perpendicular to the rotation axis, or horizontal clinorotation (HC), with the longitudinal seedling axis parallel to the rotation axis (see Figure 1 ) [ 36 ].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Graviresponse and Biological Effects of Vertical and Horizontal Clinorotation in Arabidopsis thaliana Root Tip

Villacampa

Sora

Herranz

et al. 2021

Plants

View full text Add to dashboard Cite

Clinorotation was the first method designed to simulate microgravity on ground and it remains the most common and accessible simulation procedure. However, different experimental settings, namely angular velocity, sample orientation, and distance to the rotation center produce different responses in seedlings. Here, we compare A. thaliana root responses to the two most commonly used velocities, as examples of slow and fast clinorotation, and to vertical and horizontal clinorotation. We investigate their impact on the three stages of gravitropism: statolith sedimentation, asymmetrical auxin distribution, and differential elongation. We also investigate the statocyte ultrastructure by electron microscopy. Horizontal slow clinorotation induces changes in the statocyte ultrastructure related to a stress response and internalization of the PIN-FORMED 2 (PIN2) auxin transporter in the lower endodermis, probably due to enhanced mechano-stimulation. Additionally, fast clinorotation, as predicted, is only suitable within a very limited radius from the clinorotation center and triggers directional root growth according to the direction of the centrifugal force. Our study provides a full morphological picture of the stages of graviresponse in the root tip, and it is a valuable contribution to the field of microgravity simulation by clarifying the limitations of 2D-clinostats and proposing a proper use.

show abstract

“…1). The 2-D clinostat was used in several microgravity simulation experiments, including Arabidopsis 34 seedlings and V. natriegens 35 . Since space experiments require an extraordinary effort due to the planning, cost and experiment design, various ground-based approaches have been developed and are applied over the last centuries 36 .…”

Section: Introductionmentioning

confidence: 99%

Molecular response of Deinococcus radiodurans to simulated microgravity explored by proteometabolomic approach

Ott

Fuchs

Moeller

et al. 2019

Sci Rep

Self Cite

View full text Add to dashboard Cite

Regarding future space exploration missions and long-term exposure experiments, a detailed investigation of all factors present in the outer space environment and their effects on organisms of all life kingdoms is advantageous. Influenced by the multiple factors of outer space, the extremophilic bacterium Deinococcus radiodurans has been long-termly exposed outside the International Space Station in frames of the Tanpopo orbital mission. The study presented here aims to elucidate molecular key components in D. radiodurans, which are responsible for recognition and adaptation to simulated microgravity. D. radiodurans cultures were grown for two days on plates in a fast-rotating 2-D clinostat to minimize sedimentation, thus simulating reduced gravity conditions. Subsequently, metabolites and proteins were extracted and measured with mass spectrometry-based techniques. Our results emphasize the importance of certain signal transducer proteins, which showed higher abundances in cells grown under reduced gravity. These proteins activate a cellular signal cascade, which leads to differences in gene expressions. Proteins involved in stress response, repair mechanisms and proteins connected to the extracellular milieu and the cell envelope showed an increased abundance under simulated microgravity. Focusing on the expression of these proteins might present a strategy of cells to adapt to microgravity conditions.

show abstract

2-D Clinostat for Simulated Microgravity Experiments with Arabidopsis Seedlings

Cited by 20 publications

References 28 publications

Putative gravisensors among microtubule associated proteins

Putative gravisensors among microtubule associated proteins

Analysis of Graviresponse and Biological Effects of Vertical and Horizontal Clinorotation in Arabidopsis thaliana Root Tip

Molecular response of Deinococcus radiodurans to simulated microgravity explored by proteometabolomic approach

Contact Info

Product

Resources

About