2‐D DIGE analysis of Senegalese sole (<b><i>Solea senegalensis</i></b>) testis proteome in wild‐caught and hormone‐treated F1 fish

Forné, Ignasi; Agulleiro, Maria Josep; Asensio, Esther; Abián, Joaquín; Cerdà, Joan

doi:10.1002/pmic.200800696

Cited by 33 publications

(19 citation statements)

References 46 publications

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“…Riba 2004;Costa et al 2009a, b;Oliva et al 2010). In addition, some successful attempts using ''omic'' approaches can already been found, although most also relate to aquaculture, such as transcription analysis by cDNA microarrays and PCR techniques (Manchado et al 2008;Osuna-Jiménez et al 2009;Prieto-Á lamo et al 2009 and a few others) and proteomics (Forné et al 2009;Salas-Leiton et al 2009;Costa et al 2010).…”

Section: Introductionmentioning

confidence: 97%

Transcriptomic analyses in a benthic fish exposed to contaminated estuarine sediments through laboratory and in situ bioassays

et al. 2011

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The transcription of contaminant response-related genes was investigated in juvenile Senegalese soles exposed to sediments from three distinct sites (a reference plus two contaminated) of a Portuguese estuary (the Sado, W Portugal) through simultaneous 28-day laboratory and in situ bioassays. Transcription of cytochrome P450 1A (CYP1A), metallothionein 1 (MT1), glutathione peroxidase (GPx), catalase (CAT), caspase 3 (CASP3) and 90 kDa heat-shock protein alpha (HSP90AA) was surveyed in the liver by real-time PCR. CASP3 transcription analysis was complemented by surveying apoptosis through the TUNEL reaction. After 14 days of exposure, relative transcription was either reduced or decreased in fish exposed to the contaminated sediments, revealing a disturbance stress phase during which animals failed to respond to insult. After 28 days of exposure all genes' transcription responded to contamination but laboratory and in situ assays depicted distinct patterns of regulation. Although sediments revealed a combination of organic and inorganic toxicants, transcription of the CYP1A gene was consistently correlated to organic contaminants. Metallothionein regulation was found correlated to metallic and organic xenobiotic contamination in the laboratory and in situ, respectively. The transcription of oxidative stress-related genes can be a good indicator of general stress but caution is mandatory when interpreting the results since regulation may be influenced by multiple factors. As for MT1, HSP90 up-regulation has potential to be a good indicator for total contamination, as well as the CASP3 gene, even though hepatocyte apoptosis depicted values inconsistent with sediment contamination, showing that programmed cell death did not directly depend on caspase transcription alone.

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Section: Introductionmentioning

confidence: 97%

Transcriptomic analyses in a benthic fish exposed to contaminated estuarine sediments through laboratory and in situ bioassays

et al. 2011

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“…Much research has been performed on this species in the areas of aquaculture, marine ecology and ecotoxicology. ''Omics'' (proteomics and transcriptomics) techniques have, inclusively, been performed on Senegalese soles, although more for aquaculture-than for ecotoxicology-related studies (e.g., Forné et al 2009Forné et al , 2011Salas-Leiton et al 2009;Osuna-Jiménez et al 2009;Costa et al 2010b).…”

Section: Introductionmentioning

confidence: 99%

Hepatic proteome changes in Solea senegalensis exposed to contaminated estuarine sediments: a laboratory and in situ survey

et al. 2012

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Assessing toxicity of contaminated estuarine sediments poses a challenge to ecotoxicologists due to the complex geochemical nature of sediments and to the combination of multiple classes of toxicants. Juvenile Senegalese soles were exposed for 14 days in the laboratory and in situ (field) to sediments from three sites (a reference plus two contaminated) of a Portuguese estuary. Sediment characterization confirmed the combination of metals, polycyclic aromatic hydrocarbons and organochlorines in the two contaminated sediments. Changes in liver cytosolic protein regulation patterns were determined by a combination of two-dimensional electrophoresis with de novo sequencing by tandem mass spectrometry. From the forty-one cytosolic proteins found to be deregulated, nineteen were able to be identified, taking part in multiple cellular processes such as anti-oxidative defence, energy production, proteolysis and contaminant catabolism (especially oxidoreductase enzymes). Besides a clear distinction between animals exposed to the reference and contaminated sediments, differences were also observed between laboratory- and in situ-tested fish. Soles exposed in the laboratory to the contaminated sediments failed to induce, or even markedly down-regulated, many proteins, with the exception of a peroxiredoxin (an anti-oxidant enzyme) and a few others, when compared to reference fish. In situ exposure to the contaminated sediments revealed significant up-regulation of basal metabolism-related enzymes, comparatively to the reference condition. Down-regulation of basal metabolism enzymes, related to energy production and gene transcription, in fish exposed in the laboratory to the contaminated sediments, may be linked to sediment-bound contaminants and likely compromised the organisms' ability to deploy adequate responses against insult.

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“…This species also shows highly asynchronous spermatogenesis since once males reach sexual maturity spermatogenic activity and spermiogenesis are maintained almost all year-round, although spermiation is slightly enhanced during the annual fluctuation of female ovulation (García-Ló pez et al 2006a,b). Recent studies of sole have revealed a complex cell-type-specific and stage-specific pattern of gene expression during spermatogenesis, and that human chorionic gonadotropin (hCG) can regulate the expression of genes potentially involved in steroidogenesis, progression of spermatogenesis and germ cell maturation (Cerdà et al 2008, Forné et al 2009, Mechaly et al 2012. However, the specific actions of Fsh and Lh on gene expression during spermatogenesis in Senegalese sole remain unknown.…”

Section: Introductionmentioning

confidence: 99%

Fsh and Lh direct conserved and specific pathways during flatfish semicystic spermatogenesis

Chauvigné

Zapater

Crespo

et al. 2014

Journal of Molecular Endocrinology

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The current view of the control of spermatogenesis by Fsh and Lh in non-mammalian vertebrates is largely based on studies carried out in teleosts with cystic and cyclic spermatogenesis. Much less is known concerning the specific actions of gonadotropins during semicystic germ cell development, a type of spermatogenesis in which germ cells are released into the tubular lumen where they transform into spermatozoa. In this study, using homologous gonadotropins and a candidate gene approach, for which the genes' testicular cell-type-specific expression was established, we investigated the regulatory effects of Fsh and Lh on gene expression during spermatogenesis in Senegalese sole (Solea senegalensis), a flatfish with asynchronous and semicystic germ cell development. During early spermatogenesis, Fsh and Lh upregulated steroidogenesis-related genes and nuclear steroid receptors, expressed in both somatic and germ cells, through steroid-dependent pathways, although Lh preferentially stimulated the expression of downstream genes involved in androgen and progestin syntheses. In addition, Lh specifically promoted the expression of spermatid-specific genes encoding spermatozoan flagellar proteins through direct interaction with the Lh receptor in these cells. Interestingly, at this spermatogenic stage, Fsh primarily regulated genes encoding Sertoli cell growth factors with potentially antagonistic effects on germ cell proliferation and differentiation through steroid mediation. During late spermatogenesis, fewer genes were regulated by Fsh or Lh, which was associated with a translational and posttranslational downregulation of the Fsh receptor in different testicular compartments. These results reveal that conserved and specialized gonadotropic pathways regulate semicystic spermatogenesis in flatfish, which may spatially adjust cell germ development to maintain a continuous reservoir of spermatids in the testis.

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2‐D DIGE analysis of Senegalese sole (Solea senegalensis) testis proteome in wild‐caught and hormone‐treated F1 fish

Cited by 33 publications

References 46 publications

Transcriptomic analyses in a benthic fish exposed to contaminated estuarine sediments through laboratory and in situ bioassays

Transcriptomic analyses in a benthic fish exposed to contaminated estuarine sediments through laboratory and in situ bioassays

Hepatic proteome changes in Solea senegalensis exposed to contaminated estuarine sediments: a laboratory and in situ survey

Fsh and Lh direct conserved and specific pathways during flatfish semicystic spermatogenesis

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