2 Deep learning-based cellular image analysis for intelligent medical diagnosis

Delikoyun, Kerem; Cine, Ersin; Anıl-İnevi, Müge; Sarigil, Oyku; Özçivici, Engin; Tekin, H. Cumhur

doi:10.1515/9783110668322-002

Cited by 3 publications

(2 citation statements)

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“…Lastly, the developments in OOC technology can eliminate animal testing in preclinical studies and enable multiple drug testing on a single chip. Similarly, the integration of emerging “artificial intelligence” techniques into microfluidic-based spheroid-engineering methods by controlling spheroid size formation, evaluating new drug candidates, and analyzing drug responses could lead to the development of next-generation tools for effective drug studies. , These developments can eliminate animal tests and accelerate preclinical analyses and may lead to “clinical trials-on-a-chip” technology in the future.…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, the integration of emerging "artificial intelligence" techniques into microfluidic-based spheroid-engineering methods by controlling spheroid size formation, evaluating new drug candidates, and analyzing drug responses could lead to the development of next-generation tools for effective drug studies. 168,222 These developments can eliminate animal tests and accelerate preclinical analyses and may lead to "clinical trials-on-a-chip" technology in the future. The global 3D cell culture market including spheroid engineering is expected to grow from $1.3 billion (U.S. dollars) in 2022 to $2.6 billion by 2027, at a compounded annual growth rate (CAGR) of 15.6% between 2022 and 2027.…”

Section: Design Parameters Of Microfluidicmentioning

confidence: 99%

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Spheroid Engineering in Microfluidic Devices

et al. 2023

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cell culture techniques are commonly employed to investigate biophysical and biochemical cellular responses. However, these culture methods, having monolayer cells, lack cell−cell and cell−extracellular matrix interactions, mimicking the cell microenvironment and multicellular organization. Three-dimensional (3D) cell culture methods enable equal transportation of nutrients, gas, and growth factors among cells and their microenvironment. Therefore, 3D cultures show similar cell proliferation, apoptosis, and differentiation properties to in vivo. A spheroid is defined as self-assembled 3D cell aggregates, and it closely mimics a cell microenvironment in vitro thanks to cell−cell/matrix interactions, which enables its use in several important applications in medical and clinical research. To fabricate a spheroid, conventional methods such as liquid overlay, hanging drop, and so forth are available. However, these labor-intensive methods result in low-throughput fabrication and uncontrollable spheroid sizes. On the other hand, microfluidic methods enable inexpensive and rapid fabrication of spheroids with high precision. Furthermore, fabricated spheroids can also be cultured in microfluidic devices for controllable cell perfusion, simulation of fluid shear effects, and mimicking of the microenvironment-like in vivo conditions. This review focuses on recent microfluidic spheroid fabrication techniques and also organ-on-a-chip applications of spheroids, which are used in different disease modeling and drug development studies.

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Section: Discussionmentioning

confidence: 99%

Section: Design Parameters Of Microfluidicmentioning

confidence: 99%