2H‐Pyran‐2‐one and 2H‐Furan‐2‐one Derivatives from the Plant Endophytic Fungus Pestalotiopsis fici

Liu, Shuchun; Liu, Xiangyu; Guo, Liangdong; Che, Yongsheng; Liu, Ling

doi:10.1002/cbdv.201200361

Cited by 27 publications

(17 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Endophytic fungus Pestalotiopsis fici obtained from the branches of Camellia sinensis collected from the suburb of Hangzhou, China was the source of a new a-pyrone derivative ficipyrone A ( 7 ) ( Figure 1 ). Compound 7 showed antifungal activity against the plant pathogen Gibberella zeae with an IC 50 value of 15.9 µM (the positive control ketoconazole showed an IC 50 value of 6.02 µM) [ 17 ].…”

Section: Medicinal Plantsmentioning

confidence: 99%

Endophytic Fungi: A Source of Potential Antifungal Compounds

Deshmukh

Gupta

Prakash

et al. 2018

JoF

108

View full text Add to dashboard Cite

The emerging and reemerging forms of fungal infections encountered in the course of allogeneic bone marrow transplantations, cancer therapy, and organ transplants have necessitated the discovery of antifungal compounds with enhanced efficacy and better compatibility. A very limited number of antifungal compounds are in practice against the various forms of topical and systemic fungal infections. The trends of new antifungals being introduced into the market have remained insignificant while resistance towards the introduced drug has apparently increased, specifically in patients undergoing long-term treatment. Considering the immense potential of natural microbial products for the isolation and screening of novel antibiotics for different pharmaceutical applications as an alternative source has remained largely unexplored. Endophytes are one such microbial community that resides inside all plants without showing any symptoms with the promise of producing diverse bioactive molecules and novel metabolites which have application in medicine, agriculture, and industrial set ups. This review substantially covers the antifungal compounds, including volatile organic compounds, isolated from fungal endophytes of medicinal plants during 2013–2018. Some of the methods for the activation of silent biosynthetic genes are also covered. As such, the compounds described here possess diverse configurations which can be a step towards the development of new antifungal agents directly or precursor molecules after the required modification.

show abstract

Section: Medicinal Plantsmentioning

confidence: 99%

Endophytic Fungi: A Source of Potential Antifungal Compounds

Deshmukh

Gupta

Prakash

et al. 2018

JoF

108

View full text Add to dashboard Cite

show abstract

“…Compounds 95-97 were biologically active exhibiting antifungal activity against M. violaceum at a concentration of 0.05 mg ( Qin et al, 2010 ). A new α-pyrone (2 H -pyran-2-one), ficipyrone A (98), was isolated from solid cultures of the plant endophytic fungus Pestalotiopsis fici from the tea plant Camellia sinensis ( Liu et al, 2013 ). 98 displayed weak antifungal activity against Gibberella zeae , with an IC 50 value of 15.9 μM, compared with the positive control ketoconazole (IC 50 6.02 μM) ( Liu et al, 2013 ).…”

Section: Chemical Diversity Of Antifungal Secondary Metabolites From Endophytic Fungimentioning

confidence: 99%

“…A new α-pyrone (2 H -pyran-2-one), ficipyrone A (98), was isolated from solid cultures of the plant endophytic fungus Pestalotiopsis fici from the tea plant Camellia sinensis ( Liu et al, 2013 ). 98 displayed weak antifungal activity against Gibberella zeae , with an IC 50 value of 15.9 μM, compared with the positive control ketoconazole (IC 50 6.02 μM) ( Liu et al, 2013 ). Structural elucidation of the metabolites produced by the endophytic Phomopsis sp.…”

Section: Chemical Diversity Of Antifungal Secondary Metabolites From Endophytic Fungimentioning

confidence: 99%

Antifungal Secondary Metabolites Produced by the Fungal Endophytes: Chemical Diversity and Potential Use in the Development of Biopesticides

Zhao

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

Plant diseases caused by phytopathogenic fungi can lead to huge losses in the agricultural fields and therefore remain a continuous threat to the global food security. Chemical-based fungicides contributed significantly in securing crop production. However, indiscriminate application of fungicides has led to increased chemical resistance and potential risks to human health and environment. Thus, there is an urgent need for searching for new bioactive natural products and developing them into new biopesticides. Fungal endophytes, microorganisms that reside in the fresh tissues of living plants, are regarded as untapped sources of novel natural products for exploitation in agriculture and/or medicine. Chemical examination of endophytic fungi has yielded enormous antifungal natural products with potential use in the development of biopesticides. This review summarizes a total of 132 antifungal metabolites isolated from fungal endophytes in the past two decades. The emphasis is on the unique chemical diversity of these metabolic products, together with their relevant antifungal properties. Moreover, some “star molecules,” such as griseofulvin and trichothecene, as well as their synthetic derivatives that possess high potential as candidates of new natural fungicides, are also presented herein.

show abstract

“…Among them, chloropupukeananin was the first derivative of chlorinated pupukeanane found in fungi [8]. Compounds such as chloropupukeananin, chloropestolide A, chloropupukeanolide A and siccayne showed cytotoxic activity against human cancer cell lines [9][10][11][12][13][14][15], and pestalofones C and E exhibited good anti-fungal activity on Aspergillus fumigatus [12]. Due to the richness in metabolites, P. fici was sequenced [16] and 76 biosynthetic gene clusters (BGCs) were found using bioinformatic analysis [17].…”

Section: Introductionmentioning

confidence: 99%

An Optimized and Efficient CRISPR/Cas9 System for the Endophytic Fungus Pestalotiopsis fici

Huang

Yin

2021

JoF

View full text Add to dashboard Cite

Endophytic fungi are emerging as attractive producers of natural products with diverse bioactivities and novel structures. However, difficulties in the genetic manipulation of endophytic fungi limit the search of novel secondary metabolites. In this study, we improved the polyethylene glycol (PEG)-mediated protoplast transformation method by introducing the CRISPR/Cas9 system into endophytic fungus Pestalotiopsis fici. Using this approach, we performed genome editing such as site-specific gene insertion, dual-locus mutations, and long DNA fragment deletions in P. fici efficiently. The average efficiency for site-specific gene insertion and two-site gene editing was up to 48.0% and 44.4%, respectively. In addition, the genetic manipulation time with long DNA fragment (5–10 kb) deletion was greatly shortened to one week in comparison with traditional methods such as Agrobacterium tumefaciens-mediated transformation (ATMT). Taken together, the development of the CRISPR/Cas9 system in the endophytic fungus will accelerate the discovery of novel natural products and further biological study.

show abstract

2H‐Pyran‐2‐one and 2H‐Furan‐2‐one Derivatives from the Plant Endophytic Fungus Pestalotiopsis fici

Cited by 27 publications

References 20 publications

Endophytic Fungi: A Source of Potential Antifungal Compounds

Endophytic Fungi: A Source of Potential Antifungal Compounds

Antifungal Secondary Metabolites Produced by the Fungal Endophytes: Chemical Diversity and Potential Use in the Development of Biopesticides

An Optimized and Efficient CRISPR/Cas9 System for the Endophytic Fungus Pestalotiopsis fici

Contact Info

Product

Resources

About