2-Keto-l-gulonic acid inhibits the growth of Bacillus pumilus and Ketogulonicigenium vulgare

Zhang, Qian; Lyu, Shuxia

doi:10.1007/s11274-023-03700-6

Cited by 2 publications

(1 citation statement)

References 27 publications

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“…The presence of cytochrome c in K. vulgare couples the production of 2-KGA with the respiratory chain [27], and ROS inhibits the growth of K. vulgare and the production of 2-KGA [67]. Except for the conversion process of L-sorbose to 2-KGA and other metabolic activities that produce a lot of ROS, 2-KGA as the target product also causes oxidative stress in K. vulgare [68,69].…”

Section: Alleviation Of Oxidative Pressure In Fermentation Systemsmentioning

confidence: 99%

Symbiosis Mechanism of Associated Bacteria on 2-keto-L-gulonic Acid Production via Mixed Fermentation: A Review

Chen,

Liu,

Liu

et al. 2023

Fermentation

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Vitamin C, a water-soluble vitamin with strong reducing power, cannot be synthesized by the human body and participates in a variety of important biochemical reactions. Vitamin C is widely used in the pharmaceutical, food, health care, beverage, cosmetics, and feed industries, with a huge market demand. The classical two-step fermentation method is the mainstream technology for vitamin C production. D-sorbitol is transformed into L-sorbose by Gluconobacter oxydans in the first step of fermentation; then, L-sorbose is transformed into 2-keto-L-gulonic acid (2-KGA) by a coculture system composed of Ketogulonicigenium vulgare and associated bacteria; and finally, 2-KGA is transformed into vitamin C through chemical transformation. The conversion of L-sorbose into 2-KGA in the second fermentation step is performed by K. vulgare. However, considering the slow growth and low 2-KGA production of K. vulgare when cultured alone, it is necessary to add an associated bacteria to stimulate K. vulgare growth and 2-KGA production. Although the mechanism by which the associated bacteria promote K. vulgare growth and 2-KGA production has extensively been studied, this remains a hot topic in related fields. Based on the latest achievements and research, this review summarizes the metabolic characteristics of K. vulgare and associated bacteria and elucidates the mechanism by which the associated bacteria promote the growth and 2-KGA production of K. vulgare.

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Section: Alleviation Of Oxidative Pressure In Fermentation Systemsmentioning

confidence: 99%

Symbiosis Mechanism of Associated Bacteria on 2-keto-L-gulonic Acid Production via Mixed Fermentation: A Review

Chen,

Liu,

Liu

et al. 2023

Fermentation

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Rhodotorula mucilaginosa A8, a potential helper strain in a vitamin C microbial fermentation process

Zhang,

Liao,

Lyu

2024

J Basic Microbiol

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In the vitamin C microbial fermentation system, oxidative stress limits the growth and 2‐keto‐l‐gulonic acid (2‐KLG, the precursor of vitamin C) production of Ketogulonicigenium vulgare. Most Bacillus strains, as helper strains, have been reported to release key biomolecules to reduce oxidative stress and promote the growth and 2‐KLG production of K. vulgare. To understand the specific mechanism by which the helper strain and K. vulgare interact to reduce oxidative stress, a novel helper strain, Rhodotorula mucilaginosa A8, was used to construct a consortium in the co‐culture fermentation system. Based on the activities of the antioxidant enzymes and quantitative polymerase chain reaction (qPCR) analysis, R. mucilaginosa A8 could reduce oxidative stress and increase 2‐KLG production in K. vulgare by upregulating antioxidant enzyme activities and related gene‐expression levels. In addition, the carotenoids of R. mucilaginosa promoted 2‐KLG production in K. vulgare. Coculture of R. mucilaginosa with K. vulgare increased the yield of carotenoids. This study suggested that helper strains with the ability to reduce oxidative stress in K. vulgare would likely act as potential helper strains for facilitating 2‐KLG biosynthesis. This work could provide a theoretical basis for the search for potential helper strains for vitamin C microbial fermentation and for the construction of synthetic microbial communities to produce valuable products.

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2-Keto-l-gulonic acid inhibits the growth of Bacillus pumilus and Ketogulonicigenium vulgare

Cited by 2 publications

References 27 publications

Symbiosis Mechanism of Associated Bacteria on 2-keto-L-gulonic Acid Production via Mixed Fermentation: A Review

Symbiosis Mechanism of Associated Bacteria on 2-keto-L-gulonic Acid Production via Mixed Fermentation: A Review

Rhodotorula mucilaginosa A8, a potential helper strain in a vitamin C microbial fermentation process

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