2-O-(beta-d-Glucuronyl)-7-O-(2-amino-2-deoxy-alpha-d-glucopyranosyl)-l-glycero-d-manno-heptose:a Constituent of the Bordetella pertussis Endotoxin

Chaby, Richard; Moreau, Monique; Szabó, Ladislas

doi:10.1111/j.1432-1033.1977.tb11615.x

Cited by 12 publications

(2 citation statements)

References 11 publications

(12 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It should be mentioned critically that only a smaller part of the R. sulfoviridis lipopolysaccharide could so far be accounted for by analytical determinations. The concom-itant presence of acidic and amino sugars makes a complete hydrolysis of lipopolysaccharides a difficult task [13].…”

Section: Discussionmentioning

confidence: 99%

Lipopolysaccharide with 2,3-diamino-2,3-dideoxyglucose containing lipid A in Rhodopseudomonas sulfoviridis

Ahamed¹

1982

FEMS Microbiology Letters

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Lipopolysaccharide with 2,3-diamino-2,3-dideoxyglucose containing lipid A in Rhodopseudomonas sulfoviridis

Ahamed¹

1982

FEMS Microbiology Letters

View full text Add to dashboard Cite

“…Although a glucosamine residue with a free amino group is present in the core oligosaccharide from a strain of Shigella ,flexneri serotype 6 [46], and possibly in other oligosaccharides from Escherichia coli [47] and Bordetella pertussis [48], the closest similarity to P . maltophilia is found for Xanthomonas species [37, 49 -541.…”

Section: IVmentioning

confidence: 99%

Lipopolysaccharides from Pseudomonas maltophilia

Neal

Wilkinson

2005

European Journal of Biochemistry

View full text Add to dashboard Cite

Structural studies have been carried out on the 0-specific polysaccharide, the core oligosaccharide, and the lipid A from the lipopolysaccharide of Pseudomonas maltophilia NCTC 10257. By means of 13C nuclear magnetic resonance spectroscopy, a tetrasaccharide repeating-unit for the 0-specific polymer has been confirmed. However, the data suggest that the L-rhamnopyranosyl residue at the branching point has the c1 configuration rather than as proposed previously [Neal, D. J. and Wilkinson, S. G. (1979) Carhohydr. Res. 69, 191 -2011. The core oligosaccharide contains residues of D-glucose, D-mannose phosphate, D-galactosamine (not N-acetylated), D-galacturonic acid, and a 3-deoxyoctulosonic acid (but no aldoheptose). A partial structure for the oligosaccharide is proposed. Lipid A is based on phosphorylated glucosamine residues, with N-fatty acyl and 0-fatty acyl substituents. The major fatty acids are 9-methyldecanoic acid, 2-hydroxy-9-methyldecanoic acid, 3-hydroxy-9-methyldecanoic acid (each ester-linked), 3-hydroxydodecanoic acid, and 3-hydroxy-I I-methyldodecanoic acid (both mainly amide-linked). The results of this study provide further evidence for a relationship Although a detailed description of Pseudomonas maltophilia was published only in 1961 [l], the organism has attracted increasingly numerous and diverse investigations. Phenotypic characterisation [l -51 has shown that the spccies is rather remote from other pseudomonads, and a relationship with Xanthomonas species has been suggested by the determination of ribosomal RNA homologies [6] and by the immunological comparison of glutamine synthetases [7]. In addition to its taxonomic interest [8], P. maltophilia also commands attention from clinical microbiologists [9] because of its activity as an opportunist pathogen. Other properties which have recently been discovered include the binding of human gonadotropin [lo], the production of an extracellular ribonuclease [l I], the possession of alginolytic activity [I 21, and accumulation in the rhizosphere of cruciferous plants [I 31. Our own involvement with P. maltophilia stems from the observation [14] that the organism does not exhibit the sensitivity to EDTA that characterises Pseudomonas aeruginosa, the type species of the genus Pseudomonas.In previous studies [15, 161 of the type strain of P. maltophilia (NCTC 10257) we have carried out general analyses of the cell envelope and determined the composition of its lipopolysaccharide component. A structure for the repeatingunit of the presumptive 0-specific side-chain of the lipopolysaccharide has also been proposed. In the present paper we describe the 13C nuclear magnetic resonance spectrum of the presumptive side-chain, and the results of structural studies of the core and lipid-A regions of the lipopolysaccharide. between P. maltophilia and some Xanthomonas species. MATERIALS AND METHODS Isolation and Fractionation of LipopolysaccharideMethods used for the batch culture of Pseudomonus maltophilia NCTC 10257, the preparation of cell envelopes, the ext...

show abstract

Structure of a hexasaccharide proximal to the hydrophobic region of lipopolysaccharides present inBordetella pertussis endotoxin preparations

Lebbar

Caroff

Szabó

et al. 1994

Carbohydrate Research

View full text Add to dashboard Cite

2-O-(beta-d-Glucuronyl)-7-O-(2-amino-2-deoxy-alpha-d-glucopyranosyl)-l-glycero-d-manno-heptose:a Constituent of the Bordetella pertussis Endotoxin

Cited by 12 publications

References 11 publications

Lipopolysaccharide with 2,3-diamino-2,3-dideoxyglucose containing lipid A in Rhodopseudomonas sulfoviridis

Lipopolysaccharide with 2,3-diamino-2,3-dideoxyglucose containing lipid A in Rhodopseudomonas sulfoviridis

Lipopolysaccharides from Pseudomonas maltophilia

Structure of a hexasaccharide proximal to the hydrophobic region of lipopolysaccharides present inBordetella pertussis endotoxin preparations

Contact Info

Product

Resources

About