2-Step lectin-magnetic separation (LMS) strategy combined with AuNPs-based colorimetric system for S. aureus detection in blood

Vancomycin-conjugated polydopamine-coated magnetic nanoparticles for molecular diagnostics of Gram-positive bacteria in whole blood

“…The results suggest that the use of van-PDA-MNP eliminated the non-speci c adsorption of blood cells and the formation of aggregates in the blood, which resulted in successful removal of the PCR inhibitory compounds and improved LOD. As previously reported [23,32], van and lectin conjugated with MNPs were used to magnetically separate bacteria from blood. As the conventionally used MNPs interact with blood cells and form aggregates, they have included an extra step for treating whole blood to remove the blood cells through natural sedimentation [23] or centrifugation after they have lysed red blood cells [32].…”

“…As previously reported [23,32], van and lectin conjugated with MNPs were used to magnetically separate bacteria from blood. As the conventionally used MNPs interact with blood cells and form aggregates, they have included an extra step for treating whole blood to remove the blood cells through natural sedimentation [23] or centrifugation after they have lysed red blood cells [32]. Because of the ability of the PDA coating to prevent non-speci c adsorption of blood cells and aggregation, van-PDA-MNPs can be used to capture and preconcentrate target bacteria from whole blood without the need for additional pretreatment steps.…”

Upconversion Nanoprobes Based on a Horseradish Peroxidase-Regulated Dual-Mode Strategy for the Ultrasensitive Detection of Staphylococcus aureus in Meat

“…9 Despite the reliability of the results obtained, a considerably longer time is required and is labor-intensive. 10 These traditional detection methods are not able to respond imminently to sudden public health and food poisoning incidents. As a result, several advanced approaches have been appeared in the literature to reduce the assay time for S. aureus detection such as the real-time multiplex polymerase chain reaction (PCR), enzyme-linked immunosensor assay (ELISA), and loop-mediated isothermal amplification.…”

One-step-immunoassay of procalcitonin enables rapid and accurate diagnosis of bacterial infection

“…9,10 Because septic symptoms including fever, tachycardia, tachypnea, and leukocytosis 4,7 are not sepsis-specic, or overlap with those of other noninfectious systemic inammation, 4,11 either blood culture or detection of sepsis-specic biomarkers has been used as diagnostic assay method. [9][10][11][12] Blood culture is a direct method for conrming bacterial infection [13][14][15][16] but it takes 1 to 3 days until the assay is completed and therefore is not suitable for rapid diagnosis. Furthermore, this laborintensive method suffers from low sensitivity and may miss slow-growing or fastidious bacteria.…”

“…Furthermore, this laborintensive method suffers from low sensitivity and may miss slow-growing or fastidious bacteria. 16,17 Polymerase chain reaction (PCR)-based assay can detect bacterial cells within several hours but is not sufficiently sensitive for the blood samples containing bacteria at low concentration such as approximately 1 to 100 colony forming units. Therefore, this PCR method oen requires a culture-enrichment step, leading to delayed assay and increased cost.…”