20-hydroxyecdysone dilates muscle arterioles in a nitric oxide-dependent, estrogen ER-β receptor-independent manner

Orie, Nelson N.; Raees, Asmaa; Aljaber, Maneera Y.; Mohamed-Ali, Nada; Bensmail, Halima; Hamza, M; Al-Ansari, Nasser; Beotra, Alka; Mohamed‐Ali, Vidya; Al-Maadheed, Mohammed

doi:10.1016/j.phyplu.2021.100078

Cited by 4 publications

(3 citation statements)

References 25 publications

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“…20-Hydroxyecdysone has proved to be beneficial in vivo in a mouse model of acute lung injury [ 78 ]. Furthermore, BIO101 potentially displays anti-fibrotic, vasodilatory [ 79 ] and respiratory and cardioprotective effects associated with restoring RAS balance through MasR activation [ 65 , 80 ]. The results of these clinical trials testing BIO101 and other compounds aiming to rebalance the RAS are eagerly waited for.…”

Section: Methodsmentioning

confidence: 99%

A comparison between virus- versus patients-centred therapeutic attempts to reduce COVID-19 mortality

Camelo

Latil

Agus³

et al. 2021

Emerging Microbes & Infections

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Since December 2019, coronavirus disease 2019 (COVID-19), caused by infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has changed our lives. Elderly and those with comorbidities represent the vast majority of patients hospitalized with severe COVID-19 symptoms, including acute respiratory disease syndrome and cardiac dysfunction. Despite a huge effort of the scientific community, improved treatment modalities limiting the severity and mortality of hospitalized COVID-19 patients are still required. Here, we compare the effectiveness of virus- and patients-centred strategies to reduce COVID-19 mortality. We also discuss the therapeutic options that might further reduce death rates associated with the disease in the future. Unexpectedly, extensive review of the literature suggests that SARS-CoV-2 viral load seems to be associated neither with the severity of symptoms nor with mortality of hospitalized patients with COVID-19. This may explain why, so far, virus-centred strategies using antivirals aiming to inhibit the viral replicative machinery have failed to reduce COVID-19 mortality in patients with respiratory failure. By contrast, anti-inflammatory treatments without antiviral capacities but centred on patients, such as dexamethasone or Tocilizumab ® , reduce COVID-19 mortality. Finally, since the spike protein of SARS-CoV-2 binds to angiotensin converting enzyme 2 and inhibits its function, we explore the different treatment options focussing on rebalancing the renin-angiotensin system. This new therapeutic strategy could hopefully further reduce the severity of respiratory failure and limit COVID-19 mortality in elderly patients.

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Section: Methodsmentioning

confidence: 99%

A comparison between virus- versus patients-centred therapeutic attempts to reduce COVID-19 mortality

Camelo

Latil

Agus³

et al. 2021

Emerging Microbes & Infections

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show abstract

“…The other question we sought to answer was what effect any rise in progesterone would have on muscle arteriolar functions and whether this would alter the vasodilatory effects of 20-OHE [ 21 ]. The data showed that progesterone was able to dilate both muscle and mesenteric arterioles, as well as enhancing the vasodilatory effect of 20-OHE in these arterioles.…”

Section: Discussionmentioning

confidence: 99%

“…Although progesterone did not require nitric oxide to relax these arterioles, its presence enhanced the relaxation to 20-OHE, which mechanism is largely nitric oxide-dependent [ 21 ]. The synergy may be related to the utilization of different but complementary mechanisms of relaxation by the two compounds in these arterioles.…”

Section: Discussionmentioning

confidence: 99%

Downregulation of CYP17A1 By 20-hydroxyecdysone: Plasma Progesterone and Its Vasodilatory Properties

et al. 2022

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Aim: To investigate the effect of 20-hydroxyecdysone on steroidogenic pathway genes and plasma progesterone, and its potential impact on vascular functions. Methods: Chimeric mice with humanized liver were treated with 20-hydroxyecdysone for 3 days, and hepatic steroidogenic pathway genes and plasma progesterone were measured by transcriptomics and GC–MS/MS, respectively. Direct effects on muscle and mesenteric arterioles were assessed by myography. Results: CYP17A1 was downregulated in 20-hydroxyecdysone-treated mice compared with untreated group (p = 0.04), with an insignificant increase in plasma progesterone. Progesterone caused vasorelaxation which was blocked by 60 mM KCl, but unaffected by nitric oxide synthase inhibition. Conclusion: In the short term, 20-hydroxyecdysone mediates CYP17A1 downregulation without a significant increase in plasma progesterone, which has a vasodilatory effect involving inhibition of voltage-dependent calcium channels, and the potential to enhance 20-hydroxyecdysone vasorelaxation.

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Organs-specific metabolomics and anticholinesterase activity suggests a trade-off between metabolites for therapeutic advantages of Trillium govanianum Wall. ex D. Don

Kumar,

Kumari,

Kumar

2024

Sci Rep

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Trillium govanianum is traditionally used to treat innumerable alignments like sexual disorders, cancer, inflammation etc. Mainly rhizomes of T. govanianum have been explored for phytochemical profiling but comprehensive metabolomics of other parts has not been yet deeply investigated. Thus, current study was aimed for organs-specific (roots, rhizomes, rhizomatous buds, stems, leaves, and fruits) phytochemical profiling of T. govanianum via metabolomics approach. Targeted (steroidal saponins and free sugars) and non-targeted metabolomics were performed by UPLC-PDA/ELSD & UHPLC-Q-TOF-IMS. Among steroidal compounds, 20-hydroxyecdysone, pennogenin-3-O-β-chacotrioside, dioscin were found predominantly in all samples while diosgenin was identified only in rhizomes. Further, four free sugars viz. 2-deoxyribose (116.24 ± 1.26 mg/g: leaves), fructose (454.76 ± 12.14 mg/g: rhizomes), glucose (243.21 ± 7.53 mg/g: fruits), and galactose (69.06 ± 2.14 mg/g: fruits) were found significant in respective parts of T. govanianum. Elemental analysis of targeted samples was determined by atomic absorption spectrophotometer. Heavy metals (Cd, Hg, Pd, As) were absent while micro- (Mn, Na, Zn, Cu) and macro- (Ca, Fe, Mg, K) elements were found in all samples. Furthermore, UHPLC-Q-TOF-IMS had identified 103 metabolites based on their mass fragmentation patterns and 839 were tentatively predicted using METLIN database. The multivariate statistical analysis showed organs specific clustering and variance of metabolites. Apart from this, extracts were evaluated for in vitro anticholinesterase activity, and found potentials inhibitors with IC50 values 2.02 ± 0.15 to 27.65 ± 0.89 mg/mL and 3.58 ± 0.12 to 16.81 ± 2.48 mg/mL of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzyme, respectively. Thus, comprehensive metabolomics and anti-cholinesterase activity of different parts of T. govanianum would lay the foundation for improving medicinal importance and health benefits of T. govanianum.

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20-hydroxyecdysone dilates muscle arterioles in a nitric oxide-dependent, estrogen ER-β receptor-independent manner

Cited by 4 publications

References 25 publications

A comparison between virus- versus patients-centred therapeutic attempts to reduce COVID-19 mortality

A comparison between virus- versus patients-centred therapeutic attempts to reduce COVID-19 mortality

Downregulation of CYP17A1 By 20-hydroxyecdysone: Plasma Progesterone and Its Vasodilatory Properties

Organs-specific metabolomics and anticholinesterase activity suggests a trade-off between metabolites for therapeutic advantages of Trillium govanianum Wall. ex D. Don

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