20-Hydroxyecdysone-responsive microRNAs of insects

Jin, Xiaoli; Wu, Xiaoyan; Zhou, Lanting; He, Ting; Yin, Qiuxiang; Liu, Shiping

doi:10.1080/15476286.2020.1775395

Cited by 24 publications

(27 citation statements)

References 87 publications

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“…However, how the infection induces the upregulation of miR6498-5p is still unknown. In the upstream region of 20-hydroxyecdysone-responsive miR275 cluster, multiple ecdysone receptor elements have been found, which indicates an inducer-related induction mechanism of miRNA expression [37]. As an inhibitor, the induced upregulation of miR6498-5p may be due to the activation of host pathways involved in response to pathogen invasion.…”

Section: Discussionmentioning

confidence: 97%

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MicroRNA-6498-5p Inhibits Nosema bombycis Proliferation by Downregulating BmPLPP2 in Bombyx mori

Dong

Deng

et al. 2021

JoF

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As microRNAs (miRNAs) are important expression regulators of coding RNA, it is important to characterize their role in the interaction between hosts and pathogens. To obtain a comprehensive understanding of the miRNA alternation in Bombyx mori (B. mori) infected with Nosema bombycis (N. bombycis), RNA sequencing and stem-loop qPCR were conducted to screen and identify the significantly differentially expressed miRNAs (DEmiRNAs). A total of 17 such miRNAs were identified in response to N. bombycis infection, among which miR6498-5p efficiently inhibited the proliferation of N. bombycis in BmE-SWU1 (BmE) cells by downregulating pyridoxal phosphate phosphatase 2 (BmPLPP2). In addition, a fluorescence in situ hybridization (FISH) assay showed that miR6498-5p was located in the cytoplasm of BmE cells, while it was not found in the schizonts of N. bombycis. Further investigation of the effect of BmPLPP2 on the proliferation of schizonts found that the positive factor BmPLPP2 could facilitate N. bombycis completing its life cycle in cells by overexpression and RNAi of BmPLPP2. Our findings offer multiple new insights into the role of miRNAs in the interaction between hosts and microsporidia.

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Section: Discussionmentioning

confidence: 97%

“…PEER REVIEW 13 of 15 inducer-related induction mechanism of miRNA expression [37]. As an inhibitor, the induced upregulation of miR6498-5p may be due to the activation of host pathways involved in response to pathogen invasion.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-6498-5p Inhibits Nosema bombycis Proliferation by Downregulating BmPLPP2 in Bombyx mori

Dong

Deng

et al. 2021

JoF

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“…In insect, the moulting process is triggered by the increasing 20E concentration, and ended along with the decreasing hormone level (Kiguchi & Agui, 1981). Interestingly, the expression pattern of bmo- treatment in BmE cell (Jin et al, 2020) implying that bmo-miR-282-5p is a 20E negative response gene in the silkworm. Correspondingly, we predicted that Broad complex z1 (Br-Z1) could bind to the up regulatory region of pre-miR-282-5p (Figure S4).…”

Section: Discussionmentioning

confidence: 99%

The miR‐282‐5p regulates larval moulting process by targeting chitinase 5 in Bombyx mori

Wang

Zhang

Lai

et al. 2021

Insect Molecular Biology

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Moulting is critical for growth, development and survival in insects. As the main components of cuticle, dynamic change of chitin is consistent with the moulting process. Chitinase is the main enzyme to mediate chitin metabolism in the old cuticle. To avoid over-degrading chitin from the new cuticle, the expression of chitinase must be precisely regulated. In this study, we performed microRNA-sequencing to investigate expression change of microRNAs in silkworm epidermis during the moulting process. A comparative microRNA transcriptomic analysis from different moulting stages and 20-hydroxyecdysone (20E) treatment identified bmo-miR-282-5p as a candidate. By the bioinformatic analysis, chitinase 5 (BmCht5) was predicted to be a target of bmo-miR-282-5p. Meanwhile, a temporal expression analysis revealed that BmCht5 only expressed at moulting D3 stage, whereas bmo-miR-282-5p showed a converse pattern, in which its transcript signal disappeared at this time point. Furthermore, a luciferase assay and agomir treatment demonstrated that bmo-miR-282-5p suppressed transcript of BmCht5 in vivo. As a result, injection of 282-5p agomir triggered 40% death due to moulting failure. In addition, RNA interference (RNAi)-mediated silencing of BmCht5 caused 30% developmental defect. Taken together, our data demonstrate the coordinated regulation of chitinase 5 by conserved miR-282-5p, and the 20E signalling pathway is essential for the normal moulting process in the domesticated silkworm.

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“…Many miRNAs that respond to 20E in Drosophila and Bombyx have been identified (Jin et al ., 2020; Lim et al ., 2020), which suggests the essential role of the ecdysone pathway controlling the post‐transcriptional regulation during metamorphosis (Song & Zhou, 2020). Several ecdysone‐responsive miRNAs, including let‐7, also exhibit their anti‐metamorphic functions by targeting 20E signaling‐related genes.…”

Section: Discussionmentioning

confidence: 99%

The steroid‐induced microRNA let‐7 regulates developmental growth by targeting cdc7 in the Drosophila fat body

et al. 2020

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In insects, 20‐hydroxyecdysone (20E) limits systemic growth by triggering developmental transitions. Previous studies have shown that 20E‐induced let‐7 exhibits crosstalk with the cell cycle. Here, we examined the underlying molecular mechanisms and physiological functions of 20E‐induced let‐7 in the fat body, an organ for energy storage and nutrient mobilization which plays a critical role in the larval growth. First, the overexpression of let‐7 decreased the body size and led to the reduction of both nucleolus and cell sizes in the larval fat body. In contrast, the overexpression of let‐7‐Sponge increased the nucleolus and cell sizes. Moreover, we found that cdc7, encoding a conserved protein kinase that controls the endocycle, is a target of let‐7. Notably, the mutation of cdc7 in the fat body resulted in growth defects. Overall, our findings revealed a novel role of let‐7 in the control of endoreduplication‐related growth during larval‐prepupal transition in Drosophila.

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20-Hydroxyecdysone-responsive microRNAs of insects

Cited by 24 publications

References 87 publications

MicroRNA-6498-5p Inhibits Nosema bombycis Proliferation by Downregulating BmPLPP2 in Bombyx mori

MicroRNA-6498-5p Inhibits Nosema bombycis Proliferation by Downregulating BmPLPP2 in Bombyx mori

The miR‐282‐5p regulates larval moulting process by targeting chitinase 5 in Bombyx mori

The steroid‐induced microRNA let‐7 regulates developmental growth by targeting cdc7 in the Drosophila fat body

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