206 Genome-Wide Associations for Reproductive Traits in Russian Holstein Population

Крамаренко, А. С.; Lopukchov, A. A.; Гладырь, Е. А.; Сингина, Г. Н.; Ermilov, A. Yu.; Yanchukov, I. N.; Brem, Gottfried; Zinovieva, N. A.

doi:10.1071/rdv27n1ab206

Cited by 3 publications

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“…Thus, it is hard to be improved by using traditional selection methods, e.g., selective breeding. With the development of molecular technologies, new approaches are being applied to improve reproductive traits and other complex traits, such as marker-assisted selection (MAS) and genomic selection [ 5 , 6 ]. These methods have been used widely in domestic animals for years.…”

Section: Introductionmentioning

confidence: 99%

The microRNA miR-14 Regulates Egg-Laying by Targeting EcR in Honeybees (Apis mellifera)

Chen

2021

Insects

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Honeybees (Apis mellifera) are important pollinators and are commonly used for honey production. The oviposition behavior in honeybees is complex and errors in oviposition could affect the development of the bee colony. Recent studies reported that RNA–RNA cross-talk played a critical role in several biological processes, including reproduction. Ecdysone receptor (EcR) and miR-14 were previously reported to play important roles in egg-laying. Moreover, EcR was predicted to be the target gene of miR-14 and may form miR-14-EcR cross-talk. In this study, knocking down and overexpression of miR-14 and EcR in queen model were implemented. The effect of RNA expression of miR-14 and EcR on the number of eggs laid by honeybee queens were analyzed. Further, luciferase assay was used to confirm the target relation between miR-14 and 3′UTR of EcR. The results showed that the expression of miR-14 and EcR was associated with the number of eggs laid by queens. In specific, inhibition of miR-14 expression enhanced the number of eggs laid, while overexpression of EcR enhanced the number of eggs laid. Lastly, we determined that miR-14 directly targets the mRNA of EcR. These findings suggest that the cross-talk of miR-14-EcR plays an important role in the number of eggs laid by honeybee queens.

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Section: Introductionmentioning

confidence: 99%

The microRNA miR-14 Regulates Egg-Laying by Targeting EcR in Honeybees (Apis mellifera)

Chen

2021

Insects

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“…Thus, it is hard to introduce improvements by using traditional selection methods, eg., selective breeding. With the development of molecular technologies, new approaches applied to improve reproductive traits and other complex traits, such as marker-assisted selection (MAS) and genomic selection ( Kramarenko et al, 2014 ; Spötter et al, 2012 ). These methods have been widely used in domestic animals for years.…”

Section: Introductionmentioning

confidence: 99%

Integration of lncRNA–miRNA–mRNA reveals novel insights into oviposition regulation in honey bees

Chen

et al. 2017

PeerJ

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BackgroundThe honey bee (Apis mellifera) is a highly diverse species commonly used for honey production and pollination services. The oviposition of the honey bee queen affects the development and overall performance of the colony. To investigate the ovary activation and oviposition processes on a molecular level, a genome-wide analysis of lncRNAs, miRNAs and mRNA expression in the ovaries of the queens was performed to screen for differentially expressed coding and noncoding RNAs. Further analysis identified relevant candidate genes or RNAs.ResultsThe analysis of the RNA profiles in different oviposition phase of the queens revealed that 740 lncRNAs, 81 miRNAs and 5,481 mRNAs were differently expressed during the ovary activation; 88 lncRNAs, 13 miRNAs and 338 mRNAs were differently expressed during the oviposition inhibition process; and finally, 100 lncRNAs, four miRNAs and 497 mRNAs were differently expressed during the oviposition recovery process. In addition, functional annotation of differentially expressed RNAs revealed several pathways that are closely related to oviposition, including hippo, MAPK, notch, Wnt, mTOR, TGF-beta and FoxO signaling pathways. Furthermore, in the QTL region for ovary size, 73 differentially expressed genes and 14 differentially expressed lncRNAs were located, which are considered as candidate genes affecting ovary size and oviposition. Moreover, a core set of genes served as bridges among different miRNAs were identified through the integrated analysis of lncRNA-miRNA-mRNA network.ConclusionThe observed dramatic expression changes of coding and noncoding RNAs suggest that they may play a critical role in honey bee queens’ oviposition. The identified candidate genes for oviposition activation and regulation could serve as a resource for further studies of genetic markers of oviposition in honey bees.

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“…selective breeding. With the development of molecular technologies, new approaches applied to improve reproductive traits and other complex traits, such as marker-assisted selection (MAS) and genomic selection (Kramarenko et al 2014;Spötter et al 2012). These methods have been used widely in domestic animals for years.…”

Section: Introductionmentioning

confidence: 99%

Peer Review #2 of "Integration of lncRNA–miRNA–mRNA reveals novel insights into oviposition regulation in honey bees (v0.2)"

2017

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Background The honey bee (Apis mellifera) is a highly diverse species commonly used for honey production and pollination services. The oviposition of honey bee queen affects the development and overall performance of the colony. To investigate the ovary activation and oviposition processes on a molecular level, a genome-wide analysis of lncRNAs, miRNAs and mRNAs expression in ovaries of the queens was performed to screen for differentially expressed coding and noncoding RNAs. Further analysis identified relevant candidate genes or RNAs. Results The analysis of the RNA profiles in different oviposition phase of the queens revealed that 740 lncRNAs, 81 miRNAs and 5481 mRNAs were differently expressed during the ovary activation; 88 lncRNAs, 13 miRNAs and 338 mRNAs were differently expressed during the oviposition inhibition process; and finally, 100 lncRNAs, 4 miRNAs and 497 mRNAs were differently expressed during the oviposition recovery process. In addition, functional annotation of differentially expressed RNAs revealed several pathways that are closely related to oviposition, including hippo, MAPK, notch, Wnt, mTOR, TGF-beta and FoxO signaling pathways. Furthermore, in the QTL region for ovary size, 73 differentially expressed genes and 14 differentially expressed lncRNAs were located, which are considered as candidate genes affecting ovary size and oviposition. Moreover, a core set of genes served as bridges among different miRNAs were identified through the integrated analysis of lncRNA-miRNA-mRNA network. Conclusion The observed dramatic expression changes of coding and noncoding RNAs suggest that they may play a critical role in honey bee queens' oviposition. The identified candidate genes for oviposition activation and regulation could serve as a resource for further studies of genetic markers of oviposition in honey bees.

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206 Genome-Wide Associations for Reproductive Traits in Russian Holstein Population

Cited by 3 publications

References 0 publications

The microRNA miR-14 Regulates Egg-Laying by Targeting EcR in Honeybees (Apis mellifera)

The microRNA miR-14 Regulates Egg-Laying by Targeting EcR in Honeybees (Apis mellifera)

Integration of lncRNA–miRNA–mRNA reveals novel insights into oviposition regulation in honey bees

Peer Review #2 of "Integration of lncRNA–miRNA–mRNA reveals novel insights into oviposition regulation in honey bees (v0.2)"

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