This work was carried out to find a new and cheap material as source for alternative energy. In the present study a newly recorded bacterial sub-strain (E. coli SAY) was used for the bio-fermentation of latus leaves. The results show that the bacteria were able to fermenting the sugar presented in the latus leaves and produce ethanol. It was found that the pretreatment increases the amount of sugar available for fermentation and the best results were found with HCL pretreatment. The resulted ethanol concentrations were increased with distillation as well as with dehydration process.