210 The effect of CGMP-specifc phosphodiesterase inhibitors on oxytocin-induced myometrial contractions

Cho, Jae Sung; Laporte, Régent; Alvarez, Juan; Lim, Kee-Hak

doi:10.1016/s0002-9378(01)80245-3

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar to sildenafil, UK-363664 inhibited oxytocinevoked contractions of preterm rat myometrium and this effect decreased at term (26), the effect being demonstrated by the selective cGMP phosphodiesterase inhibitor zaprinast (27). Sensitivity to UK-114542 was significantly higher in the uterine rings from pregnant versus non-pregnant horn with no difference in maximal effects in our study.…”

Section: Discussionsupporting

confidence: 52%

Expression of iNOS mRNA and Inhibitory Effect of NO on Uterine Contractile Activity in Rats Are Determined by Local Rather Than Systemic Factors of Pregnancy

et al. 2004

View full text Add to dashboard Cite

Abstract. Our purpose was to investigate whether the local or systemic factors of pregnancy are associated with inducible nitric oxide synthase (iNOS) mRNA expression and to determine the inhibitory effects of pharmacological agents that increase cGMP levels in rat myometrium. iNOS mRNA expression was determined in uterine tissues from nonpregnant rats and on day 17 of gestation in the pregnant and non-pregnant uterine horns by RT-PCR. In addition, uterine rings from the pregnant and non-pregnant uterine horns were placed in Krebs-Henseleit solution for isometric recordings of spontaneous contractions. Concentration-inhibition relationships to diethylamine / nitric oxide complex, 8-bromo-cGMP, and the selective phosphodiesterase V inhibitor were obtained. Compared to nonpregnant rats, expression of iNOS mRNA in myometrium increased during pregnancy, which was maximal on day 17, followed by a decrease on day 21 of gestation. Expression of iNOS mRNA at day 17 of gestation was greater in pregnant uterine horns than in nonpregnant ones. Maximal inhibition of phosphodiesterase V and increasing cGMP induced similar inhibition of spontaneous contractions in nonpregnant and pregnant uterine horns, while NO induced less inhibition in the former. The results suggest that the local pregnancy factor is needed for signal transduction from NO to soluble guanylate cyclase at a time when maximal expression of iNOS mRNA is evident.

show abstract

Section: Discussionsupporting

confidence: 52%