2115 POSTER Randomized phase 3 clinical trial comparing 130-nanometer albumin bound paclitaxel with solvent-based paclitaxel in Chinese patients with metastatic breast cancer

Guan, Zhong; Feng, F.Y.; Li, L.Q.; Zefei, J.; Shen, Zhenzhou; Yu, Shuhui; Feng, Jun; Huang, Jing; Zhao, Yang; Hawkins, Michael

doi:10.1016/s1359-6349(07)70877-7

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“…[31] By increasing the polymer percentage, a viscose gel layer is formed, resisting to erosion and the diffusion of the drug is controlled primarily by the gel viscosity. [32][33][34] …”

Section: In Vitro Drug Releasementioning

confidence: 99%

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Namasivayam

2017

AJP

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Background and Purpose: Nanotechnology principles are extensively utilized in the development of various drugs which exhibit the improved spectrum of activity with the less side effects. Among the various nanoparticles (NPs), protein-based nanomaterial gains recent attention because of the high rate of efficacy, delivery, and less side effects. In this study, the antibacterial antibiotics cefpirome has been formulated with biocompatible ovalbumin (OVA) NPs and the nanodrug conjugate was evaluated against improved antibacterial activity, in vitro control release and nontarget toxicity against Vero cells and blood cells. Materials and Methods: The methods for the preparation of phycocyanin functionalized OVA NPs were optimized with various parameters adapting coacervation technique. The nanodrug conjugate prepared using the optimized condition was characterized using electron microscopy studies, Fourier transform infrared spectroscopy and in vitro controlled drug release by continuous dialysis method, antibacterial activity against human pathogenic bacterial strains by well diffusion, liquid colorimetric assays, and biofilm inhibition assays. Nontarget toxicity study was conducted by determination of cytotoxicity against Vero cell line and human peripheral blood cells under laboratory condition. Results: Phycocyanin functionalized nanodrug conjugate was prepared under optimum condition characterized by electron microscopy studies reveals nanosphere is in the range of 100-200 nm. The drug loading efficiency and entrapment efficiency were found to be 85.0% and 90.5%. The in vitro drug release profile showed a steady rate of release and maximum release of 99.0% during 20 h. Antibacterial activity against human pathogenic bacteria showed improved spectrum of activity against all the tested pathogenic bacteria as dose-dependent manner. Microtiter plate and nitrocellulose membrane assay were adapted to study the biofilm inhibition of pathogenic bacteria which revealed all the tested bacterial biofilm was highly inhibited in both the tested assays as concentration-dependent manner. Effect of drug conjugate on dynamic growth curve of tested bacteria also showed the drastic reduction of growth inhibition. The antibacterial activity of nanodrug conjugate was found to be retained all the tested temperature against all the tested bacterial organism. Nontarget toxicity studies showed there was no distinct toxic effect on Vero and blood cells. Conclusion: Effective inhibition of pathogenic bacteria and best biocompatibility of nanoformulated cefpirome would suggest the possible utilization of the formulation as an antibacterial agent to fight against human pathogenic bacteria in modern medicine.

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“…[31] By increasing the polymer percentage, a viscose gel layer is formed, resisting to erosion and the diffusion of the drug is controlled primarily by the gel viscosity. [32][33][34] …”

Section: In Vitro Drug Releasementioning

confidence: 99%

Untitled

Namasivayam

2017

AJP

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“…The nanoparticles fit into colloidal DDSs, which offer advantages of drug targeting by modified body distribution as well as the enhancement of the cellular uptake which benefits from reduction of undesired toxic side effects of the free drugs. [5][6][7] With their easy accessibility in the body, nanoparticles can be transported via the circulation to different body sites, thus aiding in systemic treatments. Nanoparticles (including nanospheres and nanocapsules of size 10-200 nm) are in the solid state and are either amorphous or crystalline.…”

Section: Introductionmentioning

confidence: 99%

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Namasivayam

2016

AJP

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Aim: In this study, algal based protein, such as phycocyanin nanoparticles loaded cephalosporine derivative cefotaxime nano drug conjugate, has been prepared for the improved antibacterial activity, in vitro drug release and cytotoxicity. Materials and Methods: Nano drug conjugate was prepared by simple coacervation technique. Method for preparation of nano drug conjugate was optimized with various parameters such as pH, ethanol to phycocyanin ratio, and crosslinking time. Well diffusion assay and turbidimetric assay were carried out to determine the antibacterial activity. Effect of nanoparticles loading on drug release was studied by continuous dialysis method, and cytotoxicity was determined by methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay using Vero cell line. Results and Discussion: Nano drug conjugate was prepared using the optimized conditions at pH were 8.0 pH, 6:1 the ethanol to phycocyanin ratio, 10 h. Crosslinking time and the nanospheres formed were characterized using scanning electron microscopy which showed a particle size of nanosphere in the range of 70-90 nm and Fourier transform infrared spectroscopy revealed the possible functional groups of nano drug conjugate. Nano drug conjugate showed distinct drug loading, entrapment efficacy, and release profile. Antibacterial activity against human pathogenic bacterial strains revealed effective inhibition at the least concentration. Biocompatibility studies using Vero cell line adopting MTT assay was confirmed by showing maximum cell viability. Conclusion: This study would suggest the possible utilization of phycocyanin nanoparticles loaded cefotaxime nano drug conjugate as an antibacterial agent against pathogenic bacteria.

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2115 POSTER Randomized phase 3 clinical trial comparing 130-nanometer albumin bound paclitaxel with solvent-based paclitaxel in Chinese patients with metastatic breast cancer

Cited by 2 publications

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