215 Cardiac Output Monitoring: Experimental Study in An Infant Shock Animal Model Comparing Pulmonary Arterial Thermodilution, Femoral Arterial Thermodilution and Bioreactance

Díez, Yaiza Beatriz Molero; Villaescusa, Javier Urbano; Garcia, M.J.; Prieto, M. Botrán; Vinciguerra, D; Bellón, José María; Cid, J. López-Herce

doi:10.1203/00006450-201011001-00215

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“…Relevant studies show that Prox1 expression is related to tumor stage and grade, suggesting that Prox1 participates in the proliferation process of lung cancer cells [ 19 , 20 ]. Therefore, the MTT method was used to detect A549 and H446 cells’ growth overexpressing Prox1 after plasmid transfection.…”

Section: Resultsmentioning

confidence: 99%

“…It’s been suggested, that Prox1 is a downstream target gene of β-catenin/TCF in colon cancer, and Prox1 can lead to enhanced cell adhesion [ 30 ]. Others have confirmed that deletion, mutation, and hyper-methylation of the Prox1 gene can lead to the occurrence of biliary tumors, esophagus cancer, and breast cancer [ 20 , 31 ]. Previous experiments have proved that Rho protein can not only directly affect the construction of cytoskeleton but also participate in gene transcription regulation [ 32 ], thus indirectly affecting cytoskeleton and movement.…”

Section: Discussionmentioning

confidence: 99%

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The association of transcription factor Prox1 with the proliferation, migration, and invasion of lung cancer

2021

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Background The current study investigates the effect of transcription factor Prox1 on the proliferation, migration, and invasion ability of lung cancer. Methods Lung cancer cell lines (A549 and H446 cells) were transfected with Prox1NAD and siRNA, respectively. Thus, the A549 and H446 cells overexpressed Prox1 after transfection of Prox1NAD plasmids, and A549 and H446 cells have low expression of Prox1 after transfection with siRNA. Reverse transcriptase quantitative PCR and western blot analyses were used to detect Prox1 mRNA and protein expression in cells. Plate clone formation experiments and MTT experiments were used to detect cell proliferation. Western blot was used to detect the expression of Rho family-related proteins in cells. Results Compared to untransfected wild-type A549 and H446 that served as blank controls, the expression level of Prox1mRNA and protein in A549 and H446 cells overexpressing Prox1 after plasmid transfection was high, while the expression level of Prox1mRNA and protein in A549 and H446 cells with low expression of Prox1 after siRNA transfection was low. With the increase of Prox1 expression, the expression of RhoA and RhoC increased, while the expression of RhoB decreased. Conclusion The finding of this study may provide a new approach for the treatment of lung cancer using targeted gene therapy.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The association of transcription factor Prox1 with the proliferation, migration, and invasion of lung cancer

2021

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215 Cardiac Output Monitoring: Experimental Study in An Infant Shock Animal Model Comparing Pulmonary Arterial Thermodilution, Femoral Arterial Thermodilution and Bioreactance

Abstract: Background: Timing to indicate pharmacological PDA closure is controversial and varies widely among NICUs.

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The association of transcription factor Prox1 with the proliferation, migration, and invasion of lung cancer

The association of transcription factor Prox1 with the proliferation, migration, and invasion of lung cancer

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