[218] A DEAE-cellulose chromatographic separation of57Co vitamin B12 binders in normal serum

Comparison of Biochemical Properties of Human Serum B12 Binders Derived from Sephadex and DEAE‐Cellulose Chromatography

“…SUDHIR KUMAR, LEO M. MEYER & RlCHARD A. GAMS 1961), Geon-block electrophoresis (Hall & Finkler 1962), column chromatography and immunodiffusion technique of Ouchterlony (Hall & Finkler 1963), DEAE-cellulose (Hall & Finkler 1966, Retief et a1 1967, Gizis et a1 1970, Gizis & Meyer 1971, 1972, Kumar et a1 1974, and CM-cellulose ion exchange chromatography (Hall & Finkler 1965, Hom et a1 1966, Sephadex G-200 (Hom et a1 1966, Lawrence 1969, affinity chromatography (Allen & Majerus 1972 a/b/c), and more recently isoelectric focusing (Stenman & Grasbeck 1972, Stenman 1974. It is, however, of interest that no two laboratories have used identical procedures for isolating the vitamin Biz binding proteins from human serum.…”

“…The presence of at least 3 vitamin Biz binding proteins in human serum has been demonstrated (Hall & Finkler 1966, Retief et al 1967, Lawrence 1969, Gizis et a1 1970, Gullberg 1970, Bloomfield & Scott 1972, Gizis & Meyer 1971, 1972, Chanarin et a1 1972, Kumar et a1 1974. These binders have been designated as TC I, TC I1 and TC 111.…”