227 A transcriptomic quest to untangle equine oocyte maturation

Fuente, A. de la; Meyers, S.; Dini, P.

doi:10.1071/rdv35n2ab227

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“…A previous study by A. de la Fuente characterized the transcriptome profile of oocytes (OC) and cumulus cells (CC) derived from immature (MI) and in vitro‐matured (MII) cumulus‐oocyte complexes (COC) using ordinary RNA‐seq and found that the up‐regulated differentially expressed genes (DEG) in OC‐MII were mainly involved in the regulation of cellular metabolic processes, component organization and biogenesis, and nucleic acid‐binding, whereas the down‐regulated DEGs are related to organelle, nuclear and membrane‐enclosed lumen, nucleoplasm, and nucleolus (de la Fuente et al, 2021). The dynamics of gene expression patterns in equine cumulus cells during in vivo maturation (IVV) and in vitro maturation (IVM) were further characterized in another research using RNA‐seq (de la Fuente et al, 2023). While using the Smart‐seq2, our study provides more information about the expression patterns of mRNAs and lncRNAs in equine GV and MII oocytes.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analyses reveal transcriptional profiles of horse oocytes before and after in vitro maturation

Song,

Jiang,

et al. 2023

Reprod Domestic Animals

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Oocyte in vitro maturation is necessary for the study and application of animal‐assisted reproduction technology in animal reproduction and breeding. The comprehensive transcriptional profile of equine oocyte maturated in vitro has not been fully mined yet, which makes many key transcriptional events still unidentified. Here, Smart‐seq2 was performed to analyse the gene expression pattern and the underlying regulatory mechanism of horse germinal vesicle (GV) and in vitro metaphase II (MII) oocytes. The results showed that 6402 genes (2640 up‐regulated and 3762 down‐regulated in MII samples compared to GV) and 4021 lncRNA transcripts (1210 up‐regulated and 2811 down‐regulated in MII samples compared to GV) were differentially expressed in GV and MII oocytes. Further, GO and KEGG analysis found that differentially expressed mRNAs and lncRNAs were mainly enriched in the pathways related to energy and lipid metabolism. In addition, LGALS3 was found a key gene in mediating the regulation of oocyte meiosis recovery and fertilization ability. This study provides novel knowledge about gene expression and energy metabolism during equine oocyte maturation and a reference for the further study and application of assisted reproductive technology in horse reproduction and breeding.

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Section: Discussionmentioning

confidence: 99%