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Chen, Wusi; Lennox, Sandra; Palmer, Kenneth E.; Thomson, Jennifer A.

doi:10.1023/a:1018659430217

Cited by 10 publications

(3 citation statements)

References 33 publications

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“…sanguinalis tissue culture and transformation. The tissue culture, transformation and regeneration of D. sanguinalis, including details of tissue-culture media used, have been described previously (Chen et al, 1998). For this work, embryogenic D. sanguinalis calli were transformed by particle bombardment using the Bio-Rad/ DuPont PDS1000-He system.…”

Section: Methodsmentioning

confidence: 99%

“…(6.2 MPa), each shot delivering~333 ng per plasmid. Seven days after bombardment, calli were placed on regeneration medium with selection (3 mg bialophos l 21 ) and cultured as described by Chen et al (1998). Shooting calli were transferred to rooting medium with selection and plantlets were hardened off in a 33 : 33 : 33 mix of sand, compost and palm peat and finally transferred to potting soil.…”

Section: Methodsmentioning

confidence: 99%

“…Quantitative transient-replication assays in maize suspension cells co-bombarded with an infectious MSV genomic plasmid and various rep constructs were used to identify trans-dominant mutations that might inhibit MSV replication in maize. Digitaria sanguinalis, an MSV-sensitive grass (Chen et al, 1998), was then transformed with the most promising of these mutant rep constructs and a number of MSV-resistant lines, some of which were both phenotypically normal and fertile, were characterized.…”

Section: Introductionmentioning

confidence: 99%

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Inhibition of maize streak virus (MSV) replication by transient and transgenic expression of MSV replication-associated protein mutants

Shepherd

Mangwende

Martin

et al. 2007

Journal of General Virology

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Maize streak disease is a severe agricultural problem in Africa and the development of maize genotypes resistant to the causal agent, Maize streak virus (MSV), is a priority. A transgenic approach to engineering MSV-resistant maize was developed and tested in this study. A pathogenderived resistance strategy was adopted by using targeted deletions and nucleotide-substitution mutants of the multifunctional MSV replication-associated protein gene (rep). Various rep gene constructs were tested for their efficacy in limiting replication of wild-type MSV by co-bombardment of maize suspension cells together with an infectious genomic clone of MSV and assaying replicative forms of DNA by quantitative PCR. Digitaria sanguinalis, an MSV-sensitive grass species used as a model monocot, was then transformed with constructs that had inhibited virus replication in the transient-expression system. Challenge experiments using leafhopper-transmitted MSV indicated significant MSV resistance -from highly resistant to immune -in regenerated transgenic D. sanguinalis lines. Whereas regenerated lines containing a mutated full-length rep gene displayed developmental and growth defects, those containing a truncated rep gene both were fertile and displayed no growth defects, making the truncated gene a suitable candidate for the development of transgenic MSV-resistant maize.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Inhibition of maize streak virus (MSV) replication by transient and transgenic expression of MSV replication-associated protein mutants

Shepherd

Mangwende

Martin

et al. 2007

Journal of General Virology

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Plant Transformation Technology: Particle Bombardment

Twyman

Christou

2004

Handbook of Plant Biotechnology

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Stable gene transfer to plants can be achieved by two general strategies— Agrobacterium ‐mediated transformation and the direct introduction of DNA into the plant cell. Agrobacterium ‐mediated transformation is dependent on complex biological interactions between the bacterium and host plant, while direct DNA transfer is based on chemical or physical principles. Techniques included in this category, however, require particular cell types as transformation targets, and it is not always possible to regenerate plants from such cells. Only particle bombardment, the introduction of DNA into plants using high‐velocity microprojectiles, has the potential to transform any cell type from any plant, providing a truly universal transformation strategy. In this chapter, we discuss the principles of particle bombardment and variables that must be optimised for efficient transient and stable transformation. We also discuss the fate of exogenous DNA once it has entered the plant cell, and how this influences transgene expression. A literature survey is presented, listing all the plant species that have been transformed using this method, up to the end of the year 2001.

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The Use of African Indigenous Genes in the Development of Transgenic Maize Tolerant to Drought and Resistant to Maize Streak Virus

Thomson

Shepherd

Rybicki

2014

Biotechnology in Africa

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When developing a plant with resistance to biotic and abiotic stresses and utilizing genetic engineering, why should scientists limit themselves to genes from known organisms? Why not test those from indigenous species that might have unique properties? In this chapter we describe the use of indigenous genes for the development of crops important to Africa. The first is maize tolerant to drought, a situation which appears to be worsening on the continent, and the second is maize resistant to the African-endemic maize streak virus. The genes for drought tolerance were derived from the resurrection plant, Xerophyta viscosa, which survives even when it contains only 5 % of its relative water content. The plant can be 'resurrected' within 80 h of receiving moisture. Two methods were used to identify potential genes of interest. The first was complementation by functional sufficiency in Escherichia coli, resulting in the isolation of XvSap1 (which was found to code for a membrane-associated signalling protein) and XvAld, coding for aldose reducatase which converts glucose to sorbitol, an osmoprotectant. The second method was differential screening of expression libraries resulting in the isolation of XvPrx2, which codes for an antioxidant peroxiredoxin, and XvG6, which codes for a stress-responsive regulatory protein. Other genes isolated, tested, and not used further are also mentioned. For resistance to maize streak virus, the approach of pathogen derived resistance was used, resulting in the isolation of dominant negative mutants of the viral replication associated protein gene, rep. In a refinement of this approach, a virus-inducible version of the mutants was developed as well as an siRNA approach. As the development of transgenic maize is a lengthy process, the genes were first tested in model systems. For drought tolerance the

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Cited by 10 publications

References 33 publications

Inhibition of maize streak virus (MSV) replication by transient and transgenic expression of MSV replication-associated protein mutants

Inhibition of maize streak virus (MSV) replication by transient and transgenic expression of MSV replication-associated protein mutants

Plant Transformation Technology: Particle Bombardment

The Use of African Indigenous Genes in the Development of Transgenic Maize Tolerant to Drought and Resistant to Maize Streak Virus

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