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Mudasir, Mudasir; Yoshioka, Naoki; Inoue, Hidenari

doi:10.1023/a:1006906208569

Cited by 65 publications

(53 citation statements)

References 12 publications

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“…Many techniques, such as UV-Vis spectrophotometry, 15 fluorescence spectroscopy, 16 circular 17 and linear dichroism spectroscopy, 18 mass spectrometry, 19 X-ray crystallography 20 and voltammetry 21 have been used to study the binding properties between metal complexes and DNA.…”

Section: Introductionmentioning

confidence: 99%

Study on the interaction between morin-bi(III) complex and DNA with the use of methylene blue dye as a fluorophor probe

Ensafi

Hajian

Ebrahimi

2009

J. Braz. Chem. Soc.

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Com base em nossa investigação, ambos os complexos, morin-Bi(III) e Morin, podem vincularse ao DNA, embora a natureza da ligação seja diferente para cada um deles. Na presença e ausência do DNA, o morin-Bi(III) mostrou características espectrais diferentes, o que está de acordo com as observadas para outros intercaladores. Neste trabalho, a interação do complexo morin-Bi(III) com o DNA de timo de vitela foi investigada com o uso do azul de metileno (MB), como uma sonda de corante espectral e aplicação de espectrofotometria UV-Vis, espectroscopia de fluorescência e voltametria cíclica. , enquanto que o morin liga-se por um modelo de não-intercalação.Based on our investigation, although both morin-Bi(III) complex and morin can bind to DNA, the nature of the binding was found to be different for each of them. In the presence and absence of the DNA, the morin-Bi(III) complex shows different spectral characteristics which agree with those observed for other intercalators. In this work, the interaction of morin-Bi(III) complex with calf thymus DNA was investigated with the use of methylene blue (MB) dye as a spectral probe and application of UV-Vis spectrophotometry, fluorescence spectroscopy and cyclic voltammetry. The 2:1 morin-Bi(III) complex ratio was calculated by UV-Vis spectroscopy (mole ratio method). The fluorescence signal of Bi(III)-morin complex is increased with DNA addition whereas the fluorescence signal of Morin is decreased with DNA addition. The fluorescence signal of the DNA-complex is quenched by addition of MB which confirms the displacement of the complex with MB. Cyclic voltammetry studies confirm the intercalation reaction. The results showed that only morin-Bi(III) complex can intercalate into the double helix of the DNA. The apparent binding constant of morin-Bi(III) complex with DNA is found to be 2.8 × 10 4 L mol -1, while morin binds in a non-intercalation mode.

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Section: Introductionmentioning

confidence: 99%

Study on the interaction between morin-bi(III) complex and DNA with the use of methylene blue dye as a fluorophor probe

Ensafi

Hajian

Ebrahimi

2009

J. Braz. Chem. Soc.

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“…1, 26) [DNA]/(e a Ϫe f )ϭ Fluorescence Spectra Further support for the complexes binding to DNA by intercalation mode was given through the competitive binding experiment. EB is a conjugate planar molecule.…”

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confidence: 99%

Antitumor Activity and DNA-Binding Investigations of the Zn(II) and Cu(II) Complexes with Isoeuxanthone

Wang

Shen

2009

CHEMICAL & PHARMACEUTICAL BULLETIN

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Recently, more and more attention has been paid to xanthones that are widely present as a class of secondary metabolites in some higher plants and microorganisms. [1][2][3] These naturally occurring compounds and their synthetic analogs have been reported to exhibit multiple important pharmacological properties, such as anti-oxidant, anti-inflammatory, anti-malarial and anti-cancer activity, etc. [4][5][6][7][8][9] As shown in Fig. 1a, xanthones having three linear fused aromatic rings may be viewed as flavone derivatives in which the phenyl group is fused with the two aromatic rings. This structural 'similarity,' together with the aforementioned studies on hydroxyflavones and biological efficacy of xanthones enforced many scientists to isolate or synthesize appropriately modified derivatives for the development of prospective new drug candidates.10-12) Isoeuxanthone (Fig. 1b) is one of well-known xanthone derivatives which have diverse biological profiles.Since DNA is an important cellular receptor, many chemicals exert their antitumor effects to DNA thereby changing the replication of DNA and inhibiting the growth of the tumor cell, which is the basis of designing new and more efficient antitcancer drugs and their effectiveness depends on the mode and affinity of the binding.13) Similarly, interactions of metallic antitumor reagents with DNA are also very important in understanding the mechanisms of their antitumor activities.14,15) We were thus motivated to investigate metallic complexes of isoeuxanthone on their binding modes and affinities to DNA. Therefore, in this context, we report the synthesis and characterization of the mononuclear Zn(II) complex (1) and Cu(II) complex (2) with isoeuxanthone. The interactions of them with calf thymus (ct DNA) have also been studied by absorption spectroscopy, fluorescence spectroscopy, circular dichroism spectroscopy and viscosity measurements. In addition, they were evaluated for their cytotoxic activities toward human esophageal cancer (ECA109) and human gastric cancer (SGC7901) cells by microculture tetrazolium (MTT) method. ExperimentalMaterials The ligand HL (isoeuxanthone, namely 1,6-dihydroxyxanthone) was prepared according to the literature 16) with some improvement. All the chemicals were reagent grade and were used without further purification. Calf thymus DNA (ct DNA) and ethidium bromide (EB) were obtained from Sigma Chemical Co. All the measurements involving the interactions of the two complexes with ct DNA were carried out in doubly distilled water buffer containing 5 mM Tris[Tris(hydroxymethyl)-aminomethane] and 50 mM NaCl, and adjusted to pH 7.1 with hydrochloric acid. UV-Vis spectrometer was employed to check a solution of ct DNA purity (A 260 : A 280 Ͼ1.80) and the concentration (eϭ6600 M Ϫ1 cm Ϫ1 at 260 nm) in the buffer. 17,18)Physical Measurements Elemental analyses were conducted using an Elementar Vario EL elemental analyzer. The metal contents of the complexes were determined by titration with EDTA (ethylenediamine tetraacetic acid). Infrared ...

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“…The program SMART was used to collect the intensity data, 12) SAINT for integration of the intensity, 18) SADABS for absorption correction and SHELXTL for structure solution and refinements on F 2 . 19,20) The phenyl ring was disordered into two positions with occupancy ratio 63 : 37.…”

Section: Dna Binding Experiments Methodsmentioning

confidence: 99%

“…12) . The K b value obtained here is higher than the other mononuclear or dinuclear Ni(II) complexes reported before.…”

Section: Dna-binding Studiesmentioning

confidence: 99%

Synthesis, Crystal Structure, Antioxidant Activity, and DNA-Binding Studies of a Novel Ni(II) [2*2] Grid Complex with a Rigid Bistridentate Schiff Base Ligand

Jia

et al. 2010

CHEMICAL & PHARMACEUTICAL BULLETIN

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It is well known that deoxyribonucleic acid (DNA) plays an important role in the life process since it contains all the genetic information for cellular function.1) Investigations of the interactions of DNA with transition metal complexes are basis to design new types of the pharmaceutical molecules to elucidate the mechanism involved in the site specific recognition of DNA and to determine the principles governing the recognition.2,3) Schiff bases exhibit remarkable biological activity and play an important role in bioinorganic chemistry. For example, the Cr(III) complexes can lead to DNA damage, plasmid cleavage, and protein cleavage. 4,5) The acid hydrazides possess R-CO-NH-NH 2 , a class of Schiff base, their corresponding aroylhydrazones, R-CO-NH-NϭCH-RЈ, and the dependence of their mode of chelation with transition metal ions present in the living system have been of significant interest. 6) Various coordination compounds of aroylhydrazones have been reported to act as enzyme inhibitors and are useful due to their pharmacological applications. [7][8][9] Nickel is recognized as an essential trace element for bacteria, plants, animals, and humans, though the role of this metal in animal biochemistry is still not well defined.10) These facts encouraged us to synthesize a novel ligand NЈ,NЈ Results and DiscussionSynthesis and General Properties Complex 1 was prepared in high yield from reactions of the Schiff base ligand H 2 L in the presence of Ni(ClO 4 ) 2 · 6H 2 O. The UV-Vis investigations revealed that the spectra of the Ni(II) complex in N,N-dimethylformamide (DMF) solution have remained unaltered for the solutions and its molar conductance values have no obvious change for very freshly prepared and for over the whole experiment (24 h). It indicated that the Ni(II) complex is quite stable in solution. The molar conductivity data is in accordance with the 1 : 4 type electrolyte.Crystal Structure The crystal and experimental data is presented in Table 1. The selected bond distances and angles are presented in Table 2. Single-crystal X-ray analyses revealed that complex 1 crystallized in the space group I4/m consisting of the cationic square, [Ni 4 H 4 L 4 ] 4ϩ . As shown in Fig. 2, in which the ligand H 2 L coordinates to metal ion, it becomes negatively charged, HL Ϫ , and the ligands were divided into pairs, one of which lies above and the other below the mean plane through the four metal ions, giving a novel [2ϫ2] grids. The four Ni(II) atoms are distorted octahedral coordinated by two acyloxy oxygen, two amide, and two pyridine N atoms, evidenced by the angles O1-Ni1-N1 (154.3(2)°) and O1-Ni1-N2 (76.8(2)°). The distortion mainly originates from the extremely rigid binding pockets present within each ligand, with two coplanar five-membered chelate rings formed upon complexation, including one to the smaller-sized pyrazolate heterocycle, and the resulting proximity of the parallel ligand strands in the very compact grid array. So, four Ni(II) atoms occupy the corners of a [2ϫ2] grid with edge lengths Ni...

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Cited by 65 publications

References 12 publications

Study on the interaction between morin-bi(III) complex and DNA with the use of methylene blue dye as a fluorophor probe

Study on the interaction between morin-bi(III) complex and DNA with the use of methylene blue dye as a fluorophor probe

Antitumor Activity and DNA-Binding Investigations of the Zn(II) and Cu(II) Complexes with Isoeuxanthone

Synthesis, Crystal Structure, Antioxidant Activity, and DNA-Binding Studies of a Novel Ni(II) [2*2] Grid Complex with a Rigid Bistridentate Schiff Base Ligand

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