1989
DOI: 10.1016/0076-6879(89)70061-6
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[23] Isolation and characterization of histones

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Cited by 131 publications
(72 citation statements)
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“…Purification of H1 Histones by Gel Filtration-H1 histones were purified from histone solutions in 30 mM HCl (see above) by gel filtration (12,13). Briefly, solubilized H1 histones were applied onto a Biogel P60 column (Bio-Rad).…”
Section: Methodsmentioning
confidence: 99%
“…Purification of H1 Histones by Gel Filtration-H1 histones were purified from histone solutions in 30 mM HCl (see above) by gel filtration (12,13). Briefly, solubilized H1 histones were applied onto a Biogel P60 column (Bio-Rad).…”
Section: Methodsmentioning
confidence: 99%
“…Reconstitution of Histone Octamers-Histone octamers consisting of native H2A or uH2A were reconstituted as described previously (38,40).…”
Section: Sds-page-electrophoreticmentioning
confidence: 99%
“…However, this later separation is enormously time consuming, taking several days, and requires large amounts of starting sample (15-20 mg\ cm#) [3]. In contrast, complete fractionation of histones can be quickly achieved (1-2 h) by RP-HPLC and only microgram amounts of starting sample are required.…”
Section: Introductionmentioning
confidence: 99%