[25] Analysis of apocarotenoids and retinoids by capillary gas chromatography-mass spectrometry

Hc, Furr; Aj, Clifford; Jones, A. Daniel

doi:10.1016/0076-6879(92)13129-l

Cited by 18 publications

(9 citation statements)

References 7 publications

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“…Therefore reversed-phase high-performance liquid chromatography (HPLC) coupled with UV/visible detection is commonly used for isolation, separation, and identification of retinyl esters. − Limitations of HPLC separation and the similarity of absorption spectra, however, do not allow for unequivocal characterization. These disadvantages can be eliminated by employing mass spectrometry (MS), which provides both molecular weight information and characteristic fragment ion information helpful for structural elucidation. − …”

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“…Various kinds of ionization techniques, including electron impact (EI), − positive or negative chemical ionization (PCI, NCI), , laser desorption/ionization (LDI), and electrospray (ESI) 16 mass spectrometry have been applied for analysis of underivatized retinol and related retinoids. Mass spectrometry of retinoids suffers particularly from their thermal instability.…”

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High-Performance Liquid Chromatography and Laser Desorption/Ionization Mass Spectrometry of Retinyl Esters

et al. 1997

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Laser desorption/ionization mass spectrometry (LDI-MS) at 337 nm laser wavelength was used to analyze retinol and several long-chain fatty acid esters of retinol. Employing this ionization technique helped to overcome the inherent problems resulting from thermal instability of retinyl esters which render this group of compounds rather difficult for standard ionization techniques. Mass spectra were recorded with a linear time-of-flight instrument in positive ion mode. Under these conditions, retinyl esters formed radical molecular ions (M.+) and in addition fragmented by elimination of the fatty acyl chain to uniformly form a peak at m/z = 269 u. The elimination of carbon dioxide was also observed in the spectra. The method is suitable to identify specific retinyl esters in complex mixtures of these compounds. A gradient reversed-phase high-performance liquid chromatography (HPLC) is described for the separation of retinol and 15 related fatty acid esters within 28 min. HPLC was applied to separate retinyl esters from rat liver extracts. The LDI mass spectrum of the collected HPLC fraction of rat liver extract showed the molecular parent ions of retinyl myristate, pentadecanoate, palmitoleate, palmitate, heptadecanoate, linoleate, oleate, stearate, and 3,4-didehydroretinyl palmitate. LDI-MS was found to be more appropriate than matrix-assisted laser desorption/ionization for the described analytical task.

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High-Performance Liquid Chromatography and Laser Desorption/Ionization Mass Spectrometry of Retinyl Esters

et al. 1997

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“…The diagnosis of anemia was established based on the criteria recommended by the WHO, and children with Hb < 11 g/dL 16 were considered anemic. Serine retinol was processed at the Laboratory of the Micronutrient Research Center (Centro de Investigação em Micronutrientes -CIMICRON), University Hospital Lauro Wanderley, Federal University of Paraíba (UFPB) and analyzed by the high pressure liquid chromatography (HPLC) method, according to a technique recommended by Furr et al 17 . CIMICRON is accredited by WHO as a reference for the determination of retinol.…”

Section: Methodsmentioning

confidence: 99%

Estado nutricional de crianças em amamentação exclusiva prolongada no Estado de Pernambuco

Azevedo¹,

Caminha²,

Cruz

et al. 2019

Rev. bras. epidemiol.

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RESUMO: Introdução: O aleitamento materno exclusivo (AME), após o sexto mês de vida, como fonte alimentar única não é recomendado. Acredita-se que não é possível suprir às necessidades calórico proteicas, de ferro e vitaminas sem a devida complementação alimentar. Objetivo: Comparar a situação nutricional de crianças com AME por mais de seis mesesversuscrianças com outras práticas de amamentação. Método: Estudo transversal/exploratório com685 crianças (39 em AME > 6meses e 646com outras práticas de amamentação). Situações de déficit antropométrico foram consideradas por valores < - 2 no escore Z, anemia avaliada por hemoglobina (Hb) < 11 g/dL e níveis deficientes/baixos de vitamina A por retinol sérico < 1,05 µmol/L. Resultados: Nãoocorreram déficitsnas relações de peso/altura, peso/idade e índice de massa corporal (IMC) no grupo de crianças em AME > 6meses, enquanto no grupo de comparação esse índice foi de aproximadamente 0,5%. O déficit na relação altura/idade foi de aproximadamente 2,6% nos dois grupos. Na relação peso/altura e no IMC, os resultados variaram de 28,7 a 31,9% para excesso de peso no grupo de comparação. As médias de Hb, retinol sérico, peso e altura foram similares nos grupos. Discussão: A baixa prevalência (≤ 0,6%) de desnutrição energético proteica (DEP) nos dois grupos representa um achado, surpreendentemente, abaixo dos valores encontrados em população de referência de normalidade internacional, padrão da Organização Mundial da Saúde (OMS). Conclusão: As crianças que se mantiveram em AME após seis meses apresentaram situação nutricional equivalente àquelas com outras práticas de amamentação.

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“…The samples were then stored in a freezer at −20 • C and were later transported to the Micronutrients Research Centre Paraíba Federal University (UFPB), kept cold at all times. Concentrations of serum retinol were analysed by high performance liquid chromatography (HPLC, model 305, Gilson, France) using the technique established by Furr et al [17]. The intra-and inter-assay variations for low and high concentrations of retinol ranged from 2.4 to 6.7 for intra-assay and 4.3 to 8.5 for inter-assay replication.…”

Section: Methods and Evaluation Techniquesmentioning

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The Effects of Two Maternal Vitamin A Supplementation Regimens on Serum Retinol in Postpartum Mothers: A Randomised Controlled Trial in Brazil

et al. 2012

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Objective. To test whether the serum retinol level in mothers supplemented with 400,000 IU of vitamin A is higher than in those supplemented with 200,000 IU and to estimate duration of the protective effect of vitamin A supplementation in the serum retinol level. Methods. Double-blind, randomised controlled trial performed in two hospitals in the state of Pernambuco in northeast Brazil. Three hundred twelve mothers were recruited immediately postpartum. All women received a capsule containing 200,000 IU of vitamin A, and 10 days after delivery, they were randomly assigned to one of two treatment groups. One group received a second capsule containing vitamin A and the other group received a placebo. Each group was invited back after 2, 4, and 6 months for serum retinol analyses. Results. No difference was found between the two groups in serum maternal retinol concentration at 2 months (2.13 versus 2.03 μmol/L), 4 months (2.20 versus 2.24 μmol/L) or 6 months (2.29 versus 2.31 μmol/L). Because there was no further effect and because this population has a level of vitamin A deficiency considered mild, our results do not support a proposal to increase the dosing schedule for vitamin A in postpartum women as recommended by the IVACG.

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[25] Analysis of apocarotenoids and retinoids by capillary gas chromatography-mass spectrometry

Cited by 18 publications

References 7 publications

High-Performance Liquid Chromatography and Laser Desorption/Ionization Mass Spectrometry of Retinyl Esters

High-Performance Liquid Chromatography and Laser Desorption/Ionization Mass Spectrometry of Retinyl Esters

Estado nutricional de crianças em amamentação exclusiva prolongada no Estado de Pernambuco

The Effects of Two Maternal Vitamin A Supplementation Regimens on Serum Retinol in Postpartum Mothers: A Randomised Controlled Trial in Brazil

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