25 Characterization of Lipids of Halophilic Archaea

Corcelli, Angela; Lobasso, Simona

doi:10.1016/s0580-9517(08)70028-x

Cited by 47 publications

(48 citation statements)

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“…The strains used for comparisons were cultured according to the authors' description for each strain and standardizing to the same incubation conditions. Polar lipids were extracted with chloroform/methanol following the method for extraction of membrane polar lipids of halophilic archaea described by Corcelli & Lobasso (2006); the extracts were dried carefully using a SpeedVac Thermo Savan SPD111V before weighing and then dissolved in chloroform to obtain a concentration of 10 mg lipid ml …”

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confidence: 99%

Halorubrum halodurans sp. nov., an extremely halophilic archaeon isolated from a hypersaline lake

Corral

Haba

Sánchez‐Porro

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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Two extremely halophilic archaea, strains Cb34T and C170, belonging to the genus Halorubrum, were isolated from the brine of the hypersaline lake Aran-Bidgol in Iran. Cells of the two strains were motile, pleomorphic rods, stained Gram-variable and produced redpigmented colonies. Strains Cb34 T and C170 required 25 % (w/v) salts, pH 7.0 and 37 8C for optimal growth under aerobic conditions; 0.3 M Mg 2+ was required. Cells of both isolates were lysed in distilled water and hypotonic treatment with ,10 % NaCl provoked cell lysis. Phylogenetic analysis based on 16S rRNA gene sequence similarities showed that these two strains were closely related to Halorubrum cibi B31 T (98.8 %) and other members of the genusHalorubrum. In addition, studies based on the rpoB9 gene revealed that strains Cb34 T and C170 are placed among the species of Halorubrum and are closely related to Halorubrum cibi B31 T , with rpoB9 gene sequence similarity less than or equal to 95.7 %. The polar lipid patterns of both strains consisted of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and sulfated mannosyl glucosyl diether. The DNA G+C content was 62.1-62.4 mol%. DNA-DNA hybridization studies confirmed that strains Cb34 T and C170 constitute a distinct species. Data obtained in this study show that the two strains represent a novel species, for which the name Halorubrum halodurans sp. nov. is proposed. The type strain is Cb34 T (5CECT 8745 T 5IBRC-M 10233 T ).The haloarchaea are a group of extremely halophilic, aerobic archaea included in the class Halobacteria ) that comprises 48 genera including the genus Halorubrum, established by McGenity & Grant (1995). Currently, it is the largest haloarchaeal genus, with 31 validly published species names (Parte, 2015). Species of the genus Halorubrum have been isolated from diverse natural and artificial hypersaline environments such as salterns, salt lakes, coastal sabkhas, soda lakes, saline soils and salt-fermented and salt-preserved food. It is often reported as the dominant genus present in many hypersaline environments; their ubiquity and adaptability to nearly all possible hypersaline conditions has been demonstrated by cultivation and culture-independent methodologies (Ghai et al., 2011; Makhdoumi-Kakhki et al., 2012a; Fernández et al., 2014a, b). Species of this genus are aerobic, chemo-organotrophic and require high concentrations of NaCl in media (e.g. 1.0-5.2 M) for growth. The genus is phenotypically highly variable: its members use many carbon substrates including a wide range of sugars as sources of energy. However, some species use only single carbon sources for growth. They produce bacterioruberin carotenoid pigments that colour colonies pink to red. Most described species are neutrophilic, but some are alkaliphilic and grow optimally at pH 9.5. The major polar lipids are C 20 C 20 and sometimes C 20 C 25 Modern classification of the haloarchaea requires using phenotypic characterization, a chemotaxonomic approach based on the polar l...

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confidence: 99%

Halorubrum halodurans sp. nov., an extremely halophilic archaeon isolated from a hypersaline lake

Corral

Haba

Sánchez‐Porro

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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“…At 82 h, the culture was stopped and the total fermented liquid (1.5 L) was centrifuged at 4500 rpm and 4°C for 1 h. Pigmented lipids were extracted from cells by mixing the pellet and 100 mL of CHCl 3 ∕methanol∕H 2 O (2:1:0.8 v/v), using magnetic stirring at 150 rpm for 2 h. The pigmented lipid phase (chloroform phase) was recovered by decantation and resuspended in a fresh mixture of CHCl 3 ∕methanol∕H 2 O (2:1:0.8 v/v) [17,33]. Chloroform was evaporated and the lipid pigments were solubilized with 5 mL of hexane and stored at −4°C in glass vials covered with aluminum, until the carotenoid assays by RS.…”

Section: Samples Preparation and Carotenoids Extraction From H Marismentioning

confidence: 99%

“…The pigments responsible for these colors comprise isoprenoid derivatives or compounds of retinal proteins [15,16], as found in H. dombrowskii, Halococcus morrhuae, H. salinarum NRC-1, H. salinarum (DSM670, DSM3754 T ), Halobacterium noricense, H. japonica, Halorubrum saccharovorum, Halorubrum sp. Naxos II, all with a deep pink or red pigmentation [2,17]. The pigment responsible for the purple color is a complex retinal protein called bacterio-rhodopsin or rhodopsin [18].…”

Section: Introductionmentioning

confidence: 99%

“…The pigment responsible for the purple color is a complex retinal protein called bacterio-rhodopsin or rhodopsin [18]. The bright red-pink color is conferred by isoprenoid derivatives, carotenoid pigments and bacterioruberin, which are found in the membrane of halophilic archaea [1,17]. They play not only a role in the photoresist system [3,17], but they are also important for the adjustment of the fluidity of the membrane in the changing osmotic conditions of media which they proliferate [19,20].…”

Section: Introductionmentioning

confidence: 99%

“…The bright red-pink color is conferred by isoprenoid derivatives, carotenoid pigments and bacterioruberin, which are found in the membrane of halophilic archaea [1,17]. They play not only a role in the photoresist system [3,17], but they are also important for the adjustment of the fluidity of the membrane in the changing osmotic conditions of media which they proliferate [19,20]. The search for metabolites of industrial interest in archaea is a scarcely explored field, due to the difficulty of culturing archaea (natural highly corrosive salt-rich culture media), which means these organisms cannot be cultivated in bioreactors containing stainless steel, due to the high cost of culture media, and above all, the lack of methodologies, which allow the quantification of their metabolites, including biomass.…”

Section: Introductionmentioning

confidence: 99%

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Estimation of bacterioruberin by Raman spectroscopy during the growth of halophilic archaeon Haloarcula marismortui

et al. 2014

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Halophilic archaea are interesting microorganisms that produce low biomass and metabolites, complicating their quantification. Raman spectroscopy (RS) is a powerful technique, which requires small samples, attractive for using in archaeal research. The objective of this work was the estimation of bacterioruberin content along with Haloarcula marismortui growth and their correlation with biomass concentration. RS was used to detect characteristic bands of bacterioruberin (vibrational modes C═CH, C─C, and C═C) in H. marismortui culture samples. The intensity of Raman spectra in bacterioruberin and the biomass concentration were adequately correlated. The highest production of bacterioruberin occurred at 60 h. RS is revealed as a reliable technique for the estimation of bacterioruberin in the biomass of H. marismortui, which could be considered as a promising qualitative and quantitative technique to assay metabolites in cell cultures.

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Nanoarchaeosomes in Drug Delivery

Romero

Morilla

2022

Nanotechnology in the Life Sciences

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25 Characterization of Lipids of Halophilic Archaea

Cited by 47 publications

References 49 publications

Halorubrum halodurans sp. nov., an extremely halophilic archaeon isolated from a hypersaline lake

Halorubrum halodurans sp. nov., an extremely halophilic archaeon isolated from a hypersaline lake

Estimation of bacterioruberin by Raman spectroscopy during the growth of halophilic archaeon Haloarcula marismortui

Nanoarchaeosomes in Drug Delivery

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