25% Human Serum Albumin Improves Hemodynamics and Prevents the Need for Nearly All Pre-Hospital Resuscitation in a Rat (Rattus Norvegicus) Model of Trauma and Hemorrhage

Abstract

Combat casualty care can be complicated by transport times exceeding the “golden hour,” with intervention and resuscitation limited to what the medic can carry. Pharmaceutical albumin comes highly saturated with non-esterified fatty acids (NEFAs). We recently showed that treatment with 25% bovine serum albumin (BSA) loaded with oleic acid (OA), but not NEFA-free BSA, improved survival for hours after severe hemorrhage and often eliminated the need for resuscitation in rats. However, it was unknown if pharmaceutical albumin, derived from human sources and loaded with caprylic acid (CA), would have the same benefits. We compared adjunct treatment with OA-saturated BSA, CA-saturated BSA, pharmaceutical human serum albumin (HSA), or a no-albumin control in a similar rat hemorrhagic shock model to determine if the three NEFA-albumin groups provided the same benefits relative to control. We found almost no significant differences among the NEFA-albumin groups in any measure. Mortality in controls was too low to allow for detection of improvement in survival, but NEFA-albumin groups had significantly improved hemodynamics, lactate clearance, and greatly reduced fluid requirements compared to controls. Contrary to expectations of “dehydration”, 25% albumins shifted little additional fluid into the vasculature. Rather, they restored protein to the auto-transfusion fluid. NEFA-albumin did not worsen lung permeability, but we observed a loss of circulating protein suggesting it may have increased overall vascular permeability. Our findings suggest that, though imperfect, 25% HSA could be a solution for resuscitation in austere conditions requiring prolonged field care.