258. The Vitamin B₁and Riboflavin of Milk

Abstract

With 5 Figures) SYNOPSIS Pait I. The application of Jansen's thiochrome lest to the estimation of vitamin B l m milk. Introduction Experimental The thiochrome test Study of factors influencing the action of takaphosphatase (takadiastase) on milk in the chemical estimation of vitamin Bj Effect of pH. Period of incubation Method of incubation Peptic digestion Tryptic digestion Comparison of the effect of takaphosphatase, pepsm and trypsin preparations on the assay of vitamin B, in milk. The effect of crystalline pepsm. Part II. The different forms of vitamin B 2 m milk. Fractionation of the vitamin Bj of milk Introduction Ultra-filtration through Cellophane. Ultra filtration through collodion membranes The extraction of vitamin B 1 from milk by trichloroacetic acid Examination of the protein residue obtained in the ultiafiltration of milk through Cellophane. Vitamin B t firmly bound to protein. Tiichloioacetic acid experiments. Fuller's earth adsorbates. Form in which cocarboxylase is piesent in full lactation milk. Part I I I Effect of stage of lactation and of season on tlic vitamin B r and 1 tboflavm content of milk.

A Study of the Composition of Sow's Milk

“…It is known (Houston, Kon & Thompson, 1940) that in cow's milk vitamin B, occurs in a 'free' form and also bound, much of it phosphorylated. It was of interest to find out the form in which the vitamin was present in sow's colostrum and milk, and for this purpose measurements were made before and after treatment with takaphosphatase.…”

The effect of mastitis on the carotenoids, vitamin A and phosphorus compounds of milk

“…The positive correlation between the phosphatase titre and the percentage of the total thiamine present in the free form (Table 4) appears to be general. It was observed by Houston et al (1940) for the milk of cows and goats, and by Braude et al (1947) for sow's milk. Chanda et al (1952) found it to exist in the milk Qf cows treated with thyroxine and thiouracil.…”

“…For estimating chloride the method of Davies (1938) and for phosphatase and the partition of phosphorus the procedures used by were adopted. Total thiamine and the partition of thiamine were determined by a slight modification (Chanda, McNaught & Owen, 1952) of the method described by Houston, Kon & Thompson (1940). The reading for each fraction was made in a Spekker fluorimeter in isobutanol as thiochrome (Jansen, 1936).…”

European Conference of the Nutrition Society

“…Henry, Houston, Kon and Osborne (1939), Houston, Kon and Thompson (1940) arid Henry, Houston, Kon and Thompson (1944) showed that the loss of nutrients in processing was negligible, except of vitamin B, and ascorbic acid. The loss of vitamin R, and ascorbic acid increased on storage.…”