26. Equilibrium vitrification of mouse embryos

Jin, Bo; Hotta, Eri; Kobayashi, Yukiko; Ito, Kaori; Egawa, Go; Seki, Shinsuke; Honda, Hiroshi; Mochida, Keiichi; Ogura, Atsuo; Edashige, Keisuke; Kasai, Magosaburo

doi:10.1016/j.cryobiol.2009.10.040

Cited by 10 publications

(18 citation statements)

References 12 publications

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“…Vitrification partially overcomes these disadvantage by avoiding intracellular ice crystal formation, high developmental recovery of warmed embryos, and ease of operation [19]. Vitrification can be performed using one of several systems such as OPS [13,35], Cryotube [8,9], Cryotop [1,38], CryoLoop [5,11], or CryoTech [7]. It is known that the cooling speed affects both survival rate and developmental potential of vitrified embryos, because ultra-rapid cooling retains cell recoverability after warming [26].…”

Section: Discussionmentioning

confidence: 99%

“…Most ultra-rapid cooling vitrification techniques require vitrification containers such as electron microscope grids [33], open pulled straws [20,35], CryoLoop [17,18], CryoTech [7], Cryotops [36,38], cryotip [36], Cryotube [21,22], or thin plastic strips [29]. These tools have improved the development of vitrification and led to the successful production of offspring from vitrified and warmed embryos [6,8]. Ultra-rapid cooling vitrification techniques induce embryo intracellular changes to the glass state but prevent crystallization because of the high cooling rate and high concentration of cryoprotectants.…”

Section: Introductionmentioning

confidence: 99%

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Efficient cryopreservation of mouse embryos by modified droplet vitrification (MDV)

Chang

et al. 2015

Cryobiology

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Efficient cryopreservation of mouse embryos by modified droplet vitrification (MDV)

Chang

et al. 2015

Cryobiology

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“…Cryopreserved embryos were also transferred into ICRstrain recipient females in some experiments. For cryopreserving IVF-derived 2-cell embryos, we used an equilibrium vitrification method as reported previously [14] with slight modifications. Briefly, after embryos were equilibrated in a medium consisting of 5% dimethyl sulfoxide and 5% ethylene glycol in phosphate-buffered medium (PB1) [15] for 3 min, they were transferred into a cryotube containing a vitrification medium: 42.5% (v/v) ethylene glycol, 17.3% (w/v) Ficoll and 1.0 M sucrose in PB1.…”

Section: Embryo Cryopreservation By Vitrificationmentioning

confidence: 99%

Efficient Production of Offspring from Japanese Wild-Derived Strains of Mice (Mus musculus molossinus) by Improved Assisted Reproductive Technologies1

Hasegawa

Mochida

Matoba

et al. 2012

Biology of Reproduction

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Because the genetic diversity of the laboratory mouse (Mus musculus) is very limited, wild-derived strains from this genus could provide invaluable experimental models for studies of mouse genetics and epigenetics such as quantitative trait locus analysis. However, such strains generally show poor reproductive performance under conventional husbandry conditions, so their use for large-scale analyses has been limited. This study was undertaken to devise assisted reproductive technologies (ARTs) for the efficient production of offspring in two wild-derived strains, MSM/Ms and JF1/Ms (Mus musculus molossinus). First, as females of these strains are poor responders to equine chorionic gonadotropin (eCG) stimulation, we examined the efficiency of superovulation by injecting anti-inhibin serum followed by human chorionic gonadotropin (hCG). Approximately four to six times more oocytes were ovulated than with eCG-hCG treatment in both strains, reaching ∼25-30 oocytes per female. Consequently, the procedures for in vitro fertilization using these superovulated oocytes and cryopreservation of embryos and spermatozoa could be optimized for both of the wild-derived strains. However, MSM/Ms embryos but not JF1/Ms embryos failed to develop to term after embryo transfer because of intrauterine death at mid to late gestation. We were able to overcome this obstacle by cotransfer of these embryos with those from laboratory strains combined with treatment of recipient females with an immunosuppressant (cyclosporin A). Thus, a series of ARTs essential for efficient production and preservation of the wild-derived strains were successfully devised. These technologies will facilitate systematic studies of mouse genetics and epigenetics using a wider range of genetic diversity than currently available in the genus Mus.

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“…Furthermore, Uechi et al also warn that vitrification may exert more harmful effects than slow controlled rate freezing [26]. However, few studies in the recent past have reported simpler and quicker methods of vitrification [7,8,16]. The present study was planned at the Advanced Centre for Treatment, Research and Education in Cancer (ACTREC) Laboratory Animal Facility with the objectives to cryopreserve preimplantation embryos of mice strains for long-term storage so as to reestablish the colonies from cryopreserved embryos.…”

Section: Introductionmentioning

confidence: 99%

An Attempt of Cryopreservation of Mouse Embryos at the ACTREC Laboratory Animal Facility in India

Thorat

Ingle

2012

Exp. Anim.

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Abstract:Cryopreservation is the long-term storage of viable cells/tissue in liquid nitrogen. The present study was conducted to freeze 8-cell-to morula-stage mouse embryos from the ACTREC Laboratory Animal Facility using a "slow freezing and fast revival" method. In all, 4,088 embryos were collected from 495 donor female mice of ten different strains. An average recovery of 8 embryos per donor mouse were recorded. Of the 4,088 embryos, 3,946 embryos of normal morphology were frozen in 173 straws. They were cooled down using a controlled-rate freezing assembly, and the straws were directly plunged into liquid nitrogen for long-term storage. Out of these 3,946 frozen embryos, 2,650 were found to be viable after fast revival. The highest survival rate, 81%, was recorded in B6D2F1 hybrid mice, whereas the lowest rate, 51%, was recorded in the S/RV/Cri-ba mutant strain. Out of 2,650 viable embryos, 2,359 embryos (89%) developed to the blastocyst stage after 24 h of incubation in a CO 2 incubator. The developed blastocysts were transferred surgically into 101 pseudopregnant female mice, of which 49 (48.5%) females were found to be pregnant. The highest percentage of pregnancy, 75%, was recorded in C57BL/6NCrl and NIH-III mice, whereas no pregnant recipients were recorded in Ptch, C3H/HeNCrl and NOD SCID mice. Based on the deliveries of these 49 females, an average of 4 young were delivered per female. Improvement in efficiency of freezing, thawing, and surgical transfer of embryos into pseudopregnant females is one of the challenges in such studies.

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26. Equilibrium vitrification of mouse embryos

Cited by 10 publications

References 12 publications

Efficient cryopreservation of mouse embryos by modified droplet vitrification (MDV)

Efficient cryopreservation of mouse embryos by modified droplet vitrification (MDV)

Efficient Production of Offspring from Japanese Wild-Derived Strains of Mice (Mus musculus molossinus) by Improved Assisted Reproductive Technologies1

An Attempt of Cryopreservation of Mouse Embryos at the ACTREC Laboratory Animal Facility in India

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