Salvia microphylla and Teucrium polium are medicinal and aromatic plants with ethnobotanical uses. The present study was conducted to investigate the chemical composition of Salvia microphylla and Teucrium polium essential oils, the secondary metabolites, and the biological activities of their infusion and methanolic (MeOH) extracts. Essential oils were extracted by hydrodistillation from shoots Salvia microphylla and Teucrium polium. Phenolic content, antioxidant and antimicrobial activities were determined. Gas chromatography-mass spectrometry (GC-MS) results showed the presence of significant qualitative and quantitative variations for the composition of the both essential oils (EO). Salvia microphylla EO were most complex and present 70 compounds with the major components were β-caryophyllene (13.32%), 1,8 cineole (11.25%), Cis p-Menthane-3-one (10.74%), and β-Selinenol (9.71%). Where in the Teucrium polium EO, 45 compounds were identified with the important components are β-cadinene (10%), β-citronellol (8.5%), Carvacrol (7.63%), and Eugenol (7.15%). Obtained results showed that both plant are very rich in secondary metabolites. Extracts isolated from Salvia microphylla presented the highest contents in the phenolic compound than extracted from Teucrium polium. The antioxidant activity data demonstrated that all extracts showed strong antioxidant and radical scavenging activities. Essential oil and methanol extracts presented a potential for antimicrobial activities against all tested microorganisms. The obtained results highlight the potential use of Salvia microphylla and Teucrium polium as possible natural antioxidant substances and sources of bioactive molecules.