“…It is best practice to use a staff chaperone, such as a medical assistant or nurse, during the anogenital examination. Chaperones play an important role in assuring the safety of both patient and provider (Maghfour, Chen, Olson, Roach, & Murina, 2021). The PNP must understand that the appearance of normal female genital anatomy is influenced by the presence of estrogen in both adolescents and infants.…”