2925 Expression of the Epithelial-Mesenchymal-Transition (EMT) markers, Twist, Slug, Snail, E- and N-cadherins and the association with clinical and pathological features of human pituitary adenomas

Wang, Jia; Zhu, Jun; Martin, Tracey Amanda; Sanders, Andrew; Yang, Xueming; Cheng, Shao-Ang; Yu, Hongjuan; Gui, Jia; Jiang, Wen Guo

doi:10.1016/s0959-8049(16)31640-9

Cited by 7 publications

(8 citation statements)

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“…Another interesting finding of the present study, probably the one of most clinical relevance for its application in personalized medicine, is the association of EMT-markers with local growth in NF-PitNETs. Although this association has been previously reported ( 18 , 39 – 41 ), we could observe that the EMT phenomenon is more present in the NF-PitNETs compared to somatotropinomas. Furthermore, EMT has been described as a cause of resistance to medical treatment in pituitary tumors, in particular to SRLs ( 20 ); thus, the evaluation of these biomarkers in NF-PitNETs would seem highly recommendable in case of considering medical treatment.…”

Section: Discussioncontrasting

confidence: 82%

“…EMT is not a binary process, and distinct intermediate cellular states have been reported ( 17 ). Epithelial-Mesenchymal Transition (EMT) has been linked to both the clinical course of NF-PitNET ( 18 , 19 ) and resistance to medical therapy in pituitary tumors ( 20 – 23 ). Despite not being fully understood, several mechanisms have been described to explain the association of EMT and the lack of response to therapy in PitNETs.…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of epithelial-mesenchymal transition and medical treatment related-genes in non-functioning pituitary neuroendocrine tumors

et al. 2023

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IntroductionDifferent medical therapies have been developed for pituitary adenomas. However, Non-Functioning Pituitary Neuroendocrine Tumors (NF-PitNET) have shown little response to them. Furthermore, epithelial-mesenchymal transition (EMT) has been linked to resistance to medical treatment in a significant number of tumors, including pituitary adenomas.MethodsWe aimed to evaluate the expression of EMT-related markers in 72 NF-PitNET and 16 non-tumoral pituitaries. To further explore the potential usefulness of medical treatment for NF-PitNET we assessed the expression of somatostatin receptors and dopamine-associated genes.ResultsWe found that SNAI1, SNAI2, Vimentin, KLK10, PEBP1, Ki-67 and SSTR2 were associated with invasive NF-PitNET. Furthermore, we found that the EMT phenomenon was more common in NF-PitNET than in GH-secreting pituitary tumors. Interestingly, PEBP1 was overexpressed in recurrent NF-PitNET, and could predict growth recurrence with 100% sensitivity but only 43% specificity. In parallel with previously reported studies, SSTR3 is highly expressed in our NF-PitNET cohort. However, SSTR3 expression is highly heterogeneous among the different histological variants of NF-PitNET with very low levels in silent corticotroph adenomas.ConclusionNF-PitNET showed an enhanced EMT phenomenon. SSTR3 targeting could be a good therapeutic candidate in NF-PitNET except for silent corticotroph adenomas, which express very low levels of this receptor. In addition, PEBP1 could be an informative biomarker of tumor regrowth, useful for predictive medicine in NF-PitNET.

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Section: Discussioncontrasting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Molecular characterization of epithelial-mesenchymal transition and medical treatment related-genes in non-functioning pituitary neuroendocrine tumors

et al. 2023

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“…They found a higher CDH2 transcript to be associated with larger tumours, when investigating all tumours together. They also found the CDH2 transcript to be higher in the non-functioning tumours than in PRL, GH and mixed-hormone secreting tumours (157). The studies points to a variable expression of N-cadherin in different subtypes of PAs.…”

Section: E-cadherin and N-cadherin In Gonadotroph Adenomas Papermentioning

confidence: 88%

Selection and validation of reliable reference genes for RT-qPCR analysis in a large cohort of pituitary adenomas

Normann

Øystese

Berg

et al. 2016

Molecular and Cellular Endocrinology

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“…The ZO-1 expression decrease and the increase in vimentin expression observed in our study may suggest a hypoxia-dependent EMT in A2780 cells. In contrast, increased Slug expression may induce spindle-like morphology by the loss of the apical–basal polarity in these cells [ 86 ].…”

Section: Discussionmentioning

confidence: 99%

Hypoxia, but Not Normoxia, Reduces Effects of Resveratrol on Cisplatin Treatment in A2780 Ovarian Cancer Cells: A Challenge for Resveratrol Use in Anticancer Adjuvant Cisplatin Therapy

Synowiec

Brodaczewska

Wcisło

et al. 2023

IJMS

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Natural compounds, such as resveratrol (Res), are currently used as adjuvants for anticancer therapies. To evaluate the effectiveness of Res for the treatment of ovarian cancer (OC), we screened the response of various OC cell lines to the combined treatment with cisplatin (CisPt) and Res. We identified A2780 cells as the most synergistically responding, thus optimal for further analysis. Because hypoxia is the hallmark of the solid tumor microenvironment, we compared the effects of Res alone and in combination with CisPt in hypoxia (pO2 = 1%) vs. normoxia (pO2 = 19%). Hypoxia caused an increase (43.2 vs. 5.0%) in apoptosis and necrosis (14.2 vs. 2.5%), reactive oxygen species production, pro-angiogenic HIF-1α (hypoxia-inducible factor-1α) and VEGF (vascular endothelial growth factor), cell migration, and downregulated the expression of ZO1 (zonula occludens-1) protein in comparison to normoxia. Res was not cytotoxic under hypoxia in contrast to normoxia. In normoxia, Res alone or CisPt+Res caused apoptosis via caspase-3 cleavage and BAX, while in hypoxia, it reduced the accumulation of A2780 cells in the G2/M phase. CisPt+Res increased levels of vimentin under normoxia and upregulated SNAI1 expression under hypoxia. Thus, various effects of Res or CisPt+Res on A2780 cells observed in normoxia are eliminated or diminished in hypoxia. These findings indicate the limitations in using Res as an adjuvant with CisPt therapy in OC.

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2925 Expression of the Epithelial-Mesenchymal-Transition (EMT) markers, Twist, Slug, Snail, E- and N-cadherins and the association with clinical and pathological features of human pituitary adenomas

Cited by 7 publications

References 0 publications

Molecular characterization of epithelial-mesenchymal transition and medical treatment related-genes in non-functioning pituitary neuroendocrine tumors

Molecular characterization of epithelial-mesenchymal transition and medical treatment related-genes in non-functioning pituitary neuroendocrine tumors

Selection and validation of reliable reference genes for RT-qPCR analysis in a large cohort of pituitary adenomas

Hypoxia, but Not Normoxia, Reduces Effects of Resveratrol on Cisplatin Treatment in A2780 Ovarian Cancer Cells: A Challenge for Resveratrol Use in Anticancer Adjuvant Cisplatin Therapy

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