All rights reserved. No part of this periodical may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, recording, or any information storage and retrieval system, without permission in writing from the publisher. Permission for printing and for reprinting the material contained herein has been obtained by the publisher. Reducing disturbance by tillage and addition of crop residues affects soil biota and their role in soil C storage. For 1 yr in a fi eld station trial in Davis, CA, these treatments were compared: no-tillage + continuous cropping, no-tillage + fallow, standard tillage + continuous cropping, and standard tillage + fallow. The continuous cropping treatment consisted of tomato (Lycopersicon esculentum Mill.)/sorghum-sudangrass [Sorghum bicolor (L.) Moench]/garbanzo (Cicer arietinum L.)/cowpea cover crop [Vigna unguiculata (L.) Walpers ssp. unguiculata]. The fallow rotation omitted the sorghum-sudangrass and cowpea cover crops. No-tillage + continuous cropping resulted in signifi cant changes in the surface layer (0-5 cm): higher microbial biomass C, more fungi as indicated by ergosterol and phospholipid fatty acid analysis, and higher soil NO 3 − in summer, and higher pH, soluble K + , and Olsen P at the end of the experiment. At lower depths (5-15 and 15-30 cm), few differences were observed. Total soil C (at 0-30 cm) was least with standard tillage + fallow, the typical management practice in the region. The soil food web, as indicated by the nematodes, did not become more complex with no-tillage + continuous cropping, contrary to expectations, possibly because higher trophic level nematodes had been eliminated after decades of cultivation. The bacterial decomposition pathway dominated the surface layer in all treatments, but, with no-tillage, opportunistic (colonizer-persistent Group 1) bacterial feeders greatly decreased with depth. Plant productivity, except for weeds, was reduced by no-tillage, especially in the garbanzo crop. By decreasing disturbance and increasing fungi, no-tillage + continuous cropping appears to have accelerated soil C storage but management alterations are needed to produce higher crop biomass in this Mediterranean-type climate.