293FT cells transduced with four transcription factors (OCT4, SOX2, NANOG, and LIN28) generate aberrant ES-like cells

Oka, Yusuke; Nakajima, Koichi; Nagao, Kōichi; Miura, Kazukiyo; Ishii, Nobuhisa; Kobayashi, Hideyuki

doi:10.46582/jsrm.0603008

Cited by 12 publications

(2 citation statements)

References 17 publications

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“…Although HEK 293 cell lines are used as kidney epithelial cell lines [66], they also express some proteins specific to immature neurons, e.g., neurofilament proteins or alphainternexin [67]. Hence, it is still unclear whether they should be considered a model of kidney cells or whether they should be considered instead as immature neurons [65,[67][68][69]. Given this information, the significant rise in IgG binding to this cell line could be attributed to both its expression of inflammatory biomarkers (neurofilament proteins [70]) and its representation of human kidney epithelium.…”

Section: Discussionmentioning

confidence: 99%

Serological Antibodies against Kidney, Liver, and Spleen Membrane Antigens as Potential Biomarkers in Patients with Immune Disorders

Hernandez-Suarez,

Diez-Martin,

Egiguren-Ortiz

et al. 2024

IJMS

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Immune disorders arise from complex genetic and environmental factors, which lead to dysregulation at the cellular and inflammatory levels and cause tissue damage. Recent research highlights the crucial role of reactive antibodies in autoimmune diseases and graft rejection, but their complex determination poses challenges for clinical use. Therefore, our study aimed to ascertain whether the presence of reactive antibodies against membrane antigens in tissues from both animal models and humans could serve as biomarkers in patients with autoimmune disorders. To address this issue, we examined the binding profile of serological antibodies against a diverse panel of cell membranes from the spleen, liver, and kidney tissues of monkeys, rats, and humans. After developing the cell membrane microarrays, human sera were immunologically assayed. The study was first conducted on sera from two groups, healthy subjects and patients with inflammatory and autoimmune disorders, and then optimized for kidney transplant patient sera. A significant increase in antibody reactivity against specific monkey kidney and spleen membranes was observed in the serum of patients with lupus nephritis, while kidney transplant patients showed a significant enhancement against human tissues and human embryonic kidney 293 cells. These results show the potential importance for clinical and basic research purposes of studying the presence of specific IgG against membrane antigens in patients’ serum as potential biomarkers of immune disorders. However, it is important to note that these results need to be verified in further studies with a larger sample size to confirm their relevance.

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Section: Discussionmentioning

confidence: 99%

Serological Antibodies against Kidney, Liver, and Spleen Membrane Antigens as Potential Biomarkers in Patients with Immune Disorders

Hernandez-Suarez,

Diez-Martin,

Egiguren-Ortiz

et al. 2024

IJMS

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“…LIN28 stands out among the iPS cell reprogramming factors in that it is not a transcription factor but rather an mRNA-binding protein. It is highly expressed in ES cells and generally associated with undifferentiated cells (Oka et al 2010;Heffernan et al 2011). The original reprogramming factor combination did not contain Lin28, but it was found to reprogram human cells in combination with NANOG, POU5F1, and SOX2 (Yu et al 2007).…”

Section: Lin28mentioning

confidence: 99%

Somatic Lineage Reprogramming

Shelby

Wernig

2021

Cold Spring Harb Perspect Biol

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Stem cell research for male infertility

Kobayashi

Nagao

Nakajima

2011

Reprod Medicine & Biology

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Stem cells have the ability both to differentiate into numerous tissues and to self-renew. Because of these unique properties, stem cells are promising candidates for use in regenerative medicine. Among stem cell types, embryonic stem (ES) cells have been the most studied; however, alternatives such as induced pluripotent stem cells or other adult stem cells are now being established. In this review, we focus on stem cell research that may have applications in treating male infertility. Stem cells with ES-like properties have been generated from adult human testis tissue. We expect that breakthroughs in stem cell research will increase our understanding of male infertility and lead to treatments in the near future.

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293FT cells transduced with four transcription factors (OCT4, SOX2, NANOG, and LIN28) generate aberrant ES-like cells

Cited by 12 publications

References 17 publications

Serological Antibodies against Kidney, Liver, and Spleen Membrane Antigens as Potential Biomarkers in Patients with Immune Disorders

Serological Antibodies against Kidney, Liver, and Spleen Membrane Antigens as Potential Biomarkers in Patients with Immune Disorders

Somatic Lineage Reprogramming

Stem cell research for male infertility

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