Untitled

Abstract

Isolation, detection and quantification of pathogenic DNA is the novel approach when it comes to the diagnosis of infectious disease. In every laboratory more especially in the waterborne pathogenic research laboratories, achieving an accurate result is always the main goal.Efficient and reliable results are not achieved using only the old conventional methods due to number of reasons. To this end, molecular methods that include real-time polymerase chain reactions (real-time PCRs) for the detection of nucleic acid stand novel. In this review, the efficiency, sensitivity and reliability of singleplex and multiplex real-time PCR are thoroughly considered. Further analysis on their contrasting validities was also briefed on. Articles were randomly selected with some specificity to the previous water microbiology studies. From the review, it is clear that both the use of singleplex and multiplex real-time PCR are always valid for the waterborne pathogen detection. However, majority of the researchers would prefer to choose multiplex over singleplex. Both has shown a considerable validity but running for multiple target in single reaction tube assured both the economic cost and saves time.