2A2-K08 All-in-One Unified Microfluidic Chip for Automation of Embryonic Cell Manipulation Based on Micro-Robotics

Abstract

We developed a chip for automation of cloning based on a new protocol of cloning. The protocol is removal of the zona pellucida at first stage which contributes to simple on-chip manipulation for automation of cloning. The cloning chip was designed to have 3 parts working for manipulation of oocyte and donor cell. The concept of design is summarized as follows. (1) The oocyte is cut into two parts. (2) The divided half oocyte is sorted with and without nucleus. (3) The half oocyte is coupled with a donor cell, and the coupled oocyte and donor cells are fused by an electrical field. For the current study all-in-one unified microfluidic chip was successfully fabricated for automation of cloning based on the new protocol using micro-robotics.

On-chip magnetically driven micro-robot for enucleation of oocyte

“…Automation of cloning technique is required for high-throughput production of processed cells with high quality and homogeneity [1]. On-chip micro-actuators driven by magnetic force, fluidic force and electrostatic force are important to achieve automation of complicated mico-manipulations under microscope.…”