2B or not 2B, that is the question: further investigations into the use of pencil as a matrix for matrix‐assisted laser desorption/ionisation

Langley, G. John; Herniman, Julie; Townell, Marc S.

doi:10.1002/rcm.2827

Cited by 49 publications

(48 citation statements)

References 17 publications

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“…To date a number of ionization techniques that belong to SALDI have been proposed,1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, ...…”

Section: Introductionmentioning

confidence: 99%

“…nanoparticles as typical, in treatable forms such as suspension liquids in order to ensure that the photon energies of the laser can act to desorp and ionize analytes 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45. Indeed, the soft ionization laser method such as the original form of MALDI also belongs to this category 1.…”

Section: Introductionmentioning

confidence: 99%

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A novel laser desorption/ionization method using through hole porous alumina membranes

Naito

Kotani

Ohmura

2018

Rapid Comm Mass Spectrometry

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RationaleA novel matrix‐free laser desorption/ionization method based on porous alumina membranes was developed. The porous alumina membranes have a two‐dimensional (2D) ordered structure consisting of closely aligned straight through holes of sub‐micron in diameter that are amenable to mass production by industrial fabrication processes.MethodsConsidering a balance between the ion generating efficiency and the mechanical strength of the membranes, the typical values for the hole diameter, open aperture ratio and membrane thickness were set to 200 nm, 50% and 5 μm, respectively. The membranes were coated with platinum on a single side that was exposed to the laser. Evaluation experiments were conducted on the feasibility of this membrane structure for an ionization method using a single peptide and mixed peptides and polyethylene glycol samples and a commercial matrix‐assisted laser desorption/ionization (MALDI) time‐of‐flight mass spectrometer in the positive ion mode.ResultsResults showed a softness of ionization and no sweet spot nature. The capillary action of the through holes with very high aspect ratio enables several loading protocols including sample impregnation from the surface opposite to the laser exposure side.ConclusionsThe feasibility study indicates that the through hole porous alumina membranes have several advantages in terms of usefulness over the conventional surface‐assisted laser desorption ionization (SALDI) methods. The proposed novel ionization method is termed Desorption Ionization Using Through Hole Alumina Membrane (DIUTHAME).

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A novel laser desorption/ionization method using through hole porous alumina membranes

Naito

Kotani

Ohmura

2018

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

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“…Additionally, samples can be stored on TLC plates for further analyses, which is not possible in other chromatography techniques. 17 Although the use of pencil lead as a MALDI matrix has been reported, 26,27 it is far from common. To our knowledge, only one article describes the use of pencil lead as a TLC-MALDI matrix, where a pencil line was pre-drawn on the TLC plate for the analysis of porphines.…”

Section: Resultsmentioning

confidence: 99%

“…17 Because powdered graphite is not easy to handle, pencil lead has recently been used as a new matrix material. 26,27 Pencil lead has also been used in the TLC separation and MALDI analysis of porphines, where a line was pre-drawn with a pencil on the TLC plate where the sample was separated, followed by laser irradiation on the pencil line to obtain mass spectra. 28 The current study describes, to our knowledge, the first reported detection of esters using TLC-MALDI-MS with a pencil lead matrix.…”

Section: Introductionmentioning

confidence: 99%

Detection of Long Alkyl Esters of Succinic and Maleic Acid Using TLC-MALDI-MS

Kim¹,

Han²,

Kim³

et al. 2011

Bulletin of the Korean Chemical Society

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Four esters of succinic and maleic acid were synthesized, separated by thin-layer chromatography (TLC), and identified using matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS). A comparison of matrix materials showed that 2,6-dihydroxybenzoic acid (2,6-DHB) yielded a greater ionization efficiency than 2,5-DHB prior to TLC separation. The location of each ester sample on the TLC plate was estimated by comparing the developed plate with a duplicate plate that had been visualized by immersion in a KMnO4 solution. Generally, mass spectra obtained from the KMnO4-visualized plate were relatively poor. Reproducible mass spectra with high peak abundance were difficult to obtain using the 2,6-DHB matrix from crude synthetic esters extracted from the TLC plates. Significant improvements in both reproducibility and sensitivity were realized by using pencil lead as the MALDI matrix. The current methodology will be beneficial to organic chemists since it can provide a guideline for simple and rapid characterization of small organic compounds.

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“…The samples were extensively diluted with 50:50 methanol/water solution to desired concentrations prior to analysis. Taking the analysis of glucose as an example, we defined a positive detection of nitrate-attached glucose ions based on the peak detection criteria of above a signal-to-noise (S/N) to 3 ( Figure 3) [29,[37][38][39]. Experimentally determined limit of detections (LODs) for the above mentioned analytes were listed in the Supplementary Table 1.…”

Section: Nedn As a Negative Ion-mode Matrix For Small Molecule Analysismentioning

confidence: 99%

N-(1-Naphthyl) Ethylenediamine Dinitrate: A New Matrix for Negative Ion MALDI-TOF MS Analysis of Small Molecules

Chen

Xiong

et al. 2012

J. Am. Soc. Mass Spectrom.

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An organic salt, N-(1-naphthyl) ethylenediamine dinitrate (NEDN), with rationally designed properties of a strong UV absorbing chromophore, hydrogen binding and nitrate anion donors, has been employed as a matrix to analyze small molecules (m/zG1000) such as oligosaccharides, peptides, metabolites and explosives using negative ion matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF MS). Compared with conventional matrixes such as α-cyano-4-hydroxycinnamic acid (CCA) and 2,5-dihydroxybenzoic acid (DHB), NEDN provides a significant improvement in detection sensitivity and yields very few matrixassociated fragment and cluster ions interfering with MS analysis. For low-molecular-weight saccharides, the lowest detection limit achieved ranges from 500 amol to 5 pmol, depending on the molecular weight and the structure of the analytes. Additionally, the mass spectra in the lower mass range (m/zG200) consist of only nitrate and nitric acid cluster ions, making the matrix particularly useful for structural identification of oligosaccharides by post-source decay (PSD) MALDI-MS. Such a characteristic is illustrated by using maltoheptaose as a model system. This work demonstrates that NEDN is a novel negative ion-mode matrix for MALDI-MS analysis of small molecules with nitrate anion attachment.

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2B or not 2B, that is the question: further investigations into the use of pencil as a matrix for matrix‐assisted laser desorption/ionisation

Cited by 49 publications

References 17 publications

A novel laser desorption/ionization method using through hole porous alumina membranes

A novel laser desorption/ionization method using through hole porous alumina membranes

Detection of Long Alkyl Esters of Succinic and Maleic Acid Using TLC-MALDI-MS

N-(1-Naphthyl) Ethylenediamine Dinitrate: A New Matrix for Negative Ion MALDI-TOF MS Analysis of Small Molecules

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