2PS049 Localization control of chemotaxis-related signaling components of Vibrio cholera(The 50th Annual Meeting of the Biophysical Society of Japan)

Abstract

Liye-cell imaging and analysis of cAMP-induced cAMP response ;n Dictyostetium using microfluidics chambers Fumihito Fukujini, Keita Kaminoi, Satoshi Sawaii'2'] (iGnadttate Schooi of' Arts andScienees, Universitv. ofTbk}'e, !Reseat'ch Center.for thmplex S.vstems Biology, Uhiversit.v (ijr 7bkyo, ]PRESTO, Japan Science and 71ichnoiog}' Agency)Transient synthesis and secretion of cyclic AMP {cAMP) induced by extracellular cAMP mediates interce]]ular signa]ing during aggregation of Dictyosteiium. The response is characterized by a fast initia] peak that appears about 1inin after exposure to an increase in extraeellular cAMP fo11owed by dose-dependent attenuation to the pre-stimu]us level. There is a]so a fast oscillatory component of about