3.15 Cell Culture Systems for Studying Biomaterial Interactions With Biological Barriers ☆

Hermanns, M.I.; Freese, C.; Anspach, L.; Grützner, V.; Pohl, C.; Unger, R.E.; Kirkpatrick, Charles James

doi:10.1016/b978-0-12-803581-8.09821-0

Cited by 2 publications

(2 citation statements)

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“…TT1 and hAELVi represent cells with an AT1-like phenotype regarding morphology and caveolae presence, although they do not display other common AT1 markers like AQP5 or, in the case of TT1, only show discontinuous tight junctions [ 38 , 39 , 40 , 41 ]. To get a human epithelial cell line representing the bronchial epithelium, for example BEAS-2B, 16HBE14o or Calu-3 are well established [ 42 ]. Indeed, there are also murine lung epithelial cell lines, namely MLE-12 or LA-4, representing the alveolar compartment.…”

Section: Culture Methods For Nm Hazard Assessmentmentioning

confidence: 99%

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Lung Organoids for Hazard Assessment of Nanomaterials

Kastlmeier

Guenther

Stoeger

et al. 2022

IJMS

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Lung epithelial organoids for the hazard assessment of inhaled nanomaterials offer a promising improvement to in vitro culture systems used so far. Organoids grow in three-dimensional (3D) spheres and can be derived from either induced pluripotent stem cells (iPSC) or primary lung tissue stem cells from either human or mouse. In this perspective we will highlight advantages and disadvantages of traditional culture systems frequently used for testing nanomaterials and compare them to lung epithelial organoids. We also discuss the differences between tissue and iPSC-derived organoids and give an outlook in which direction the whole field could possibly go with these versatile tools.

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Section: Culture Methods For Nm Hazard Assessmentmentioning

confidence: 99%

“…3 are well established [42]. Indeed, there are also murine lung epithelial cell lines, namely MLE-12 or LA-4, representing the alveolar compartment.…”

Section: Culture Methods For Nm Hazard Assessmentmentioning

confidence: 99%