3,3′-Diselenodipropionic acid immobilised gelatin gel: a biomimic catalytic nitric oxide generating material for topical wound healing application

Abstract

Nitrous oxide (NO) plays a pivotal role in wound healing process and promotes the regeneration of healthy endothelium. In this work, a simple method has been developed for fabricating a...

Fabrication of yeast β-glucan/sodium alginate/γ-polyglutamic acid composite particles for hemostasis and wound healing

“…During the past several years, multiple forms of biomaterials used for wound healing have been developed including hydrogels, sponges, and particles. [8][9][10][11] Among which, particles have attracted great attention because of their good therapeutic efficacy and low toxicity. 12,13 Particles with a porous structure show high water absorbability.…”

Smart Magnetic Nanocarriers for Codelivery of Nitric Oxide and Doxorubicin for Enhanced Apoptosis in Cancer Cells

“…The supernatant solution obtained after magnetic separation of SNO-Cy-CMNPs as well as their washing solution were used for determining the percentage of NO loaded onto the surface of the nanocarrier (by conversion of -SH to -SNO via nitrosation) by the Griess reagent assay using a UV–visible spectrophotometer. The assay was based on the reaction between nitrite, sulfanilamide, and N-1-naphthylethylenediamine dihydrochloride, which can produce a compound with maximum absorption at 540 nm. , For this, 50 μL of supernatant/washing solution was mixed with 100 μL of Griess reagent, and the mixture was incubated for 15 min under stirring at 37 °C in the dark. Then, the absorbance was measured using a microplate reader at 540 nm, and NO uptake was quantified against the standard plot prepared under a similar condition using sodium nitrite (concentration range = 0–100 μM, R 2 = 0.9991).…”

“…The assay was based on the reaction between nitrite, sulfanilamide, and N-1-naphthylethylenediamine dihydrochloride, which can produce a compound with maximum absorption at 540 nm. 22 , 40 For this, 50 μL of supernatant/washing solution was mixed with 100 μL of Griess reagent, and the mixture was incubated for 15 min under stirring at 37 °C in the dark. Then, the absorbance was measured using a microplate reader at 540 nm, and NO uptake was quantified against the standard plot prepared under a similar condition using sodium nitrite (concentration range = 0–100 μM, R 2 = 0.9991).…”

“…NO then binds to the heme iron of sGC to activate and generate cyclic guanosine monophosphate (cGMP) . This in turn activates the downstream kinases (cGMP-regulated protein kinases) and ion channels to regulate a variety of physiological processes like cardiovascular function, RBC and platelet activity, metabolic regulation, neurotransmission, host defense, and cancer. − …”

A redox active organodiselenide as an efficacious catalyst for the synthesis of oxygen-containing heterocyclic compounds

“…DSePA is an organodiselenide with a range of potential applications including as a radioprotector, peroxyl radical scavenger, and glutathione peroxidase (GPx) mimic. [8][9][10] However, its emergence as a catalyst in organic synthesis is a recent discovery, and the current study exploring its potential in this context is the first of its kind (Fig. 1).…”