3,4-DHPEA-EA from Olea Europaea L. is effective against standard and clinical isolates of Staphylococcus sp

Abstract

BackgroundThe aim of the present work was to evaluate the antibacterial effect of 3,4-DHPEA-EA (methyl-4-(2-(3,4-dihydroxyphenethoxy)-2-oxoethyl)-3-formyl-2-methyl-3,4-dihydro-2H-pyran-5-carboxylate), a derivate of oleuropein, against a range of Gram-positive bacteria, including ATCC strains, food and clinical isolates.MethodsThe minimum inhibitory concentrations (MICs) of 3,4-DHPEA-EA were determined by the broth microdilution method and the Bioscreen C.Results3,4-DHPEA-EA was effective against ATCC and clinical isolates of Staphylococcus aureus (MIC values between 125 and 250 μg/ml) and ATCC and clinical isolates of Staphylococcus epidermidis (MIC values between 7.81 and 62.5 μg/ml). No significant differences were observed between the two solvents (methanol and DMSO) used to dissolve 3,4-DHPEA-EA.ConclusionsThe results obtained could be used to develop novel therapies for the treatment of skin infections. Further studies need to be performed to elucidate the formation of 3,4-DHPEA-EA by acid hydrolysis of oleuropein in the human stomach.

Efficient bioconversion of oleuropein from olive leaf extract to antioxidant hydroxytyrosol by enzymatic hydrolysis and high‐temperature degradation

“…After evaluating the scientific evidence, the European Food Safety Authority deemed that HT is a protector of low‐density lipoprotein from oxidative damage . Recently, numerous in vitro studies and animal models have established that HT can prevent many diseases, due to its anticarcinogenic , anti‐inflammatory , and antimicrobial activities. In addition, protective activities have been described against high cholesterol , metabolic diseases , antigenotoxicity, cytotoxicity, and proapoptotic effects , oxidative stress , and digestive disorders .…”

In VitroAntimicrobial Activity and Effect on Biofilm Production of a White Grape Juice (Vitis vinifera) Extract

“…We have recently shown that an extract from white grape juice could have a beneficial effect on radiocontrast medium toxicity in human renal proximal tubular cells [ 6 ] and exert neuroprotective effect in a mouse model of experimental autoimmune encephalomyelitis [ 7 ]. Due to the increased antibiotic resistance, the antimicrobial properties of natural compounds have been gaining attention: we have previously reported the antibacterial activity of polyphenols-rich natural products, including almonds [ 8 , 9 ], pistachios [ 10 ], Citrus plants [ 11 ], Vitis vinifera L. [ 12 ], Olea europaea L. [ 13 ], Citrus bergamia essential oil [ 14 ], and juice [ 15 ]. In particular, the identification of novel compounds with bactericidal rather than bacteriostatic effect has attracted interest in recent years.…”

Olive mill wastewater as a potential source of antibacterial and anti-inflammatory compounds against the food-borne pathogen Campylobacter

“…as Elenolic acid and some of its derivatives (3,4-DHPE-HDCM-EA), have also been proven to possess stronger bactericidal potential compared with Oleuropein (Thielmann et al, 2017, Bisignano et al, 2014Mosele et al, 2014;De Marco et al, 2007;Soler-Rivas et al, 2000). In addition, it was shown that mixtures of phenols and secoiridoids exert greater antibacterial activity with respect to single compounds (Obied et al, 2007).…”