3,6-O-[N-(2-Aminoethyl)-acetamide-yl]-chitosan exerts antibacterial activity by a membrane damage mechanism

Yan, Feilong; Dang, Qifeng; Liu, Chengsheng; Yan, Jingquan; Wang, Teng; Fan, Bing; Cha, Dongsu; Li, Xiaoli; Liang, Shengnan; Zhang, Zhenzhen

doi:10.1016/j.carbpol.2016.04.098

Cited by 72 publications

(28 citation statements)

References 34 publications

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“…41,45 A higher number of PI stained A. alternata spores were observed in response to BITC fumigation, with the membrane integrity of A. alternata almost completely destroyed at BITC concentration of 0.625 mM. These were similar to the values previously reported by F. Yan et al 46 Lipophilic compound accumulates in the phospholipid bilayer and alters the permeability of the plasma membrane for K + and H + , resulting in the disruption of ionic homeostasis. 47,48 A loss of membrane integrity results in the leakage of small molecules ions and electrolytes from the cells.…”

Section: Discussionsupporting

confidence: 86%

Benzyl isothiocyanate fumigation inhibits growth, membrane integrity and mycotoxin production inAlternaria alternata

Wang

et al. 2020

RSC Adv.

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Section: Discussionsupporting

confidence: 86%

Benzyl isothiocyanate fumigation inhibits growth, membrane integrity and mycotoxin production inAlternaria alternata

Wang

et al. 2020

RSC Adv.

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“…PI can enter the cell and combine with nucleic acids, exhibiting red fluorescence, once the cell membrane is destroyed. Yan, F et al [24] revealed that AACS can reduce cell viability by causing cell membrane rupture or increased permeability, thereby facilitating PI entry into cells and binding to nucleic acids. They also believed that the fluorescence was related to the concentration of AACS.…”

Section: Discussionmentioning

confidence: 99%

“…Propidium iodide staining and inverted fluorescence MICroscopy (MOTIC CHINA GROUP CO., LTD., Fujian, Xiamen, China) were used to identify cell death [24]. The cells that were grown to the logarithmic growth phase were cultured with 1 MIC limonene (1 MIC and 2 MIC) for 6 h at 37 • C and then centrifuged at 2000 rpm for 5 min.…”

Section: Determination Of Cell Death Of L Monocytogenesmentioning

confidence: 99%

Antimicrobial Susceptibility and Antibacterial Mechanism of Limonene against Listeria monocytogenes

2019

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Limonene is a monoterpenoid compound, which is founded in a lot of plants' essential oils with good antibacterial activity against food-borne pathogens, but it has an ambiguous antimicrobial susceptibility and mechanism against Listeria monocytogenes (L. monocytogenes). In this study, the antimicrobial susceptibility of Limonene to L. monocytogenes was studied, and some new sights regarding its antibacterial mechanism were further explored. Scanning electron MICroscopy (SEM) verified that limonene caused the destruction of the cell integrity and wall structure of L. monocytogenes. The increase in conductivity and the leakage of intracellular biomacromolecules (nucleic acids and proteins) confirmed that limonene had an obvious effect on cell membrane permeability. The results of Propidium Iodide (PI) fluorescence staining were consistent with the results of the conductivity measurements. This indicated that limonene treatment caused damage to the L. monocytogenes cell membrane. Furthermore, the decrease in ATP content, ATPase (Na + K + -ATPase, Ca 2+ -ATPase) activity and respiratory chain complex activity indicated that limonene could hinder ATP synthesis by inhibiting the activity of the respiratory complex and ATPase. Finally, differential expression of proteins in the respiratory chain confirmed that limonene affected respiration and energy metabolism by inhibiting the function of the respiratory chain complex.Molecules 2020, 25, 33 2 of 15 terpenes in nature and is widely found in the volatile oils of various plants (black pepper, lemon and orange, etc.) [9]. Limonene has broad application prospects in antibacterial and food preservation due to its broad-spectrum bactericidal activity, safety, and low toxicity [10]. Moreover, D-limonene can significantly inhibit gram-negative and gram-positive bacteria as well as fungal activity [11]. In addition, many researchers have confirmed that D-limonene can effectively inhibit the growth of spoilage bacteria, such as Aspergillus niger, Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli [12,13]. Limonene, which is the main ingredient of lemon essential oil, was found to have antimicrobial activities against L. monocytogenes in minced beef meat [14]. However, few studies have investigated the antibacterial mechanism of limonene.The aims of this study were to determine the antibacterial susceptibility and its antibacterial mechanism of limonene against L. monocytogenes. The minimum inhibitory concentration (MIC) was used to evaluate the anti-L. monocytogenes susceptibility of limonene. The growth curves of the bacterial were determined for evaluating the effect of limonene on the growth and reproduction of L. monocytogenes. The mechanism of action of limonene was explored by analysing its influence on the cell morphology, membrane permeability and changes in the protein, nucleic acid, ATP, ATPase (Na + K + -ATPase, Ca 2+ -ATPase), respiratory chain complex I~V, and differential protein expression of the respiratory chain complex of L. monocytogenes. Resu...

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“…In addition, the intact LPS showed a gradual increase in hydrophobicity from the inside out in terms of structural composition [20], which may explain why the LPS truncation led to increased hydrophobicity in Y. enterocolitica. It is known that the adhesive capacity closely related to hydrophobicity is important for bacteria to exert their toxic effects [37]. The hydrophobicity of bacteria usually alters when the cell membrane is compromised [37].…”

Section: Discussionmentioning

confidence: 99%

Rcs Phosphorelay Responses to Truncated Lipopolysaccharide-Induced Cell Envelope Stress in Yersinia enterocolitica

Meng

Huang

et al. 2020

Molecules

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Lipopolysaccharide (LPS) is the major component of the outer membrane of Gram-negative bacteria, and its integrity is monitored by various stress response systems. Although the Rcs system is involved in the envelope stress response and regulates genes controlling numerous bacterial cell functions of Yersinia enterocolitica, whether it can sense the truncated LPS in Y. enterocolitica remains unclear. In this study, the deletion of the Y. enterocolitica waaF gene truncated the structure of LPS and produced a deep rough LPS. The truncated LPS increased the cell surface hydrophobicity and outer membrane permeability, generating cell envelope stress. The truncated LPS also directly exposed the smooth outer membrane to the external environment and attenuated the resistance to adverse conditions, such as impaired survival under polymyxin B and sodium dodecyl sulfate (SDS) exposure. Further phenotypic experiment and gene expression analysis indicated that the truncated LPS was correlated with the activation of the Rcs phosphorelay, thereby repressing cell motility and biofilm formation. Our findings highlight the importance of LPS integrity in maintaining membrane function and broaden the understanding of Rcs phosphorelay signaling in response to cell envelope stress, thus opening new avenues to develop effective antimicrobial agents for combating Y. enterocolitica infections.

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3,6-O-[N-(2-Aminoethyl)-acetamide-yl]-chitosan exerts antibacterial activity by a membrane damage mechanism

Cited by 72 publications

References 34 publications

Benzyl isothiocyanate fumigation inhibits growth, membrane integrity and mycotoxin production inAlternaria alternata

Benzyl isothiocyanate fumigation inhibits growth, membrane integrity and mycotoxin production inAlternaria alternata

Antimicrobial Susceptibility and Antibacterial Mechanism of Limonene against Listeria monocytogenes

Rcs Phosphorelay Responses to Truncated Lipopolysaccharide-Induced Cell Envelope Stress in Yersinia enterocolitica

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