[3] Application of the freeze-fracture technique to natural membranes

Fisher, Knute A.; Branton, Daniel

doi:10.1016/0076-6879(74)32006-x

Cited by 50 publications

(24 citation statements)

References 12 publications

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“…Negative staining with phosphotungstic acid was performed as described by Munn (1974). Freeze-fracture of samples was performed in a Balzers instrument (Fisher & Branton, 1974). Grids were examined in a Philips 300 electron microscope at 60 or 80 kV.…”

Section: Methodsmentioning

confidence: 99%

A procedure for membrane-protein reconstitution and the functional reconstitution of the anion transport system of the human-erythrocyte membrane

Wolosin¹

1980

Biochemical Journal

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A short procedure for the isolation of band-3 protein, the protein responsible for anion exchange in erythrocytes, in a reasonable degree of purity was developed. Using this protein preparation and a novel procedure for membrane-protein reconstitution, vesicles displaying the basic features of the anion-exchange system of the erythrocyte were obtained. The reconstitution procedure is based on slow direct removal of Triton X-100 from aqueous lipid/detergent solutions. According to the composition of the reconstitution medium, either small single-walled or large multi-walled vesicles are obtained. The procedure conserves protein properties well, as is revealed by the similarity of the rates of SQ42-exchange in erythrocytes and reconstituted vesicles when corrected for the relevant volumes. A number of functional features of the exchange system were studied and compared with those of the native membrane.

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Section: Methodsmentioning

confidence: 99%

A procedure for membrane-protein reconstitution and the functional reconstitution of the anion transport system of the human-erythrocyte membrane

Wolosin¹

1980

Biochemical Journal

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“…For a general description of fi-eeze-fracture and freeze-etch methods, results, and interpretations of results the reader is referred to numerous reviews (Akert et al, 1981;Arancia, 1983;Benedetti and Farvard, 1973;Bohler, 1975;Branton, 1966Branton, , 1971Bullivant, 1970Bullivant, , 1973Bullivant, , 1977Fineran, 1978;Fisher and Branton, 1975;Hudson et al, 1981;McNutt, 1977;Moor, 1971;Niedermeyer, 1977;Orci and Perrelet, 1975;Pfenninger, 1981;Rash and Hudson, 1979;Robards and Sletyr, 1985;Sandri et al, 1982;Shotton, 1982;Robards, 1977b, 1982;Steere, 1981;Southworth et al, 1975;Stolinski, 1977;Stolinski and Breathnach, 1975;Ververgaert, 1975, 1978;Willison and ROWS, 1980;Zingsheim, 1972;Zingsheim and Plattner, 1976). The freeze-substitution technique has also been reviewed (Bullivant, 1970;Harvey, 1982;Plattner and Bachmann, 1982;Robards and Sletyr, 1985).…”

Section: Rapid-and High Pressure-freeze Technologymentioning

confidence: 99%

A survey of ultra‐rapid cryofixation methods with particular emphasis on applications to freeze‐fracturing, freeze‐etching, and freeze‐substitution

Menco

1986

J. Elec. Microsc. Tech.

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“…6. Cardenolides appear as purple spots when sprayed first with 2 percent methanolic m-dinitrobenzoic acid and then with 5 percent methanolic KOH 10. We thank J. Roba (Continental Pharma) for performing these experiments; the details will be reported elsewhere (J. Roba, in preparation).…”

mentioning

confidence: 99%

“…Naloxone or a matched volume of physiological saline was administered intravenously in a randomized doubleblind fashion. The following items were rated on a severity scale of I to 7 (not present to severe): hallucinations, mannerisms and posturing, conceptual disorganization, unusual thought content, psychosis, and the mood variables of depression, elation, and dysphoria (10). These variables were chosen in order to evaluate the possible antipsychotic and antidepressant effects cited earlier.…”

mentioning

confidence: 99%