3-Bromopyruvate-induced glycolysis inhibition impacts larval growth and development and carbohydrate homeostasis in fall webworm, Hyphantria cunea Drury

Chitinase inhibition induces transcriptional dysregulation altering ecdysteroid-mediated control of Spodoptera frugiperda development

“…Reduced energy and trehalose content may affect insects’ vitality, leading to decreased body mass. 28 …”

Quantification of 3‐bromopyruvate in rat plasma by HPLC–MS/MS employing precolumn derivatization and the application to a pharmacokinetics study

“…The rats were divided into three groups (6-7 per group) for oral dosing of 3-BrPA (30 mg/kg) and intravenous dosing of 3-BrPA (5 mg/kg) and 3-BrPA-L-Val-L-Ile (a dipeptide prodrug of 3-BrPA, 5 mg/kg calculated as 3-BrPA), respectively. Serial blood samples (0.2 ml) were obtained via the retro-orbital plexus with a capillary tube at 5, 20, 45 min and 1, 1.5, 2, 3,4,6,8,12, 24 h after dosing. All samples were placed into heparinized tubes.…”

“…Glyceraldehyde‐3‐phosphate dehydrogenase was identified as the primary intracellular target of 3‐BrPA (Ganapathy‐Kanniappan et al, 2009; Geschwind et al, 2002). In addition to targeting glycolytic enzymes, 3‐BrPA can also inhibit several nonglycolytic enzymes (Ota et al, 2013; Qiu et al, 2021). Given the simple structure of 3‐BrPA, 3‐BrPA could be best characterized as a toxic molecule rather than a specific inhibitor of glycolysis (Sun, Sun, Huang, Hao, et al, 2020; Sun, Sun, Huang, Zhang, et al, 2020).…”