3-D axon structure extraction and analysis in confocal fluorescence microscopy images

A local maximum intensity projection (MIP) approach to the extraction of a 3D vascular network, acquired by the KnifeEdge Scanning Microscope (KESM), is presented. We build a local volume for local MIP processing at each tracing step in order to reduce the dimension of input data from 3D to 2D, which leads to a 65.22% reduction of computation time compared to 3D tracing method. The proposed method makes use of existing 2D tracing methods, extending them into a 3D tracing method. Our experimental results show that our approach can rapidly and accurately extract the medial axis of vascular data acquired by the KESM.

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A local maximum intensity projection tracing of vasculature in Knife-Edge Scanning Microscope volume data

Han

Keyser

Choe

2009

2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro

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3-D axon structure extraction and analysis in confocal fluorescence microscopy images

Cited by 1 publication

References 33 publications

A local maximum intensity projection tracing of vasculature in Knife-Edge Scanning Microscope volume data

A local maximum intensity projection tracing of vasculature in Knife-Edge Scanning Microscope volume data

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