3-D Printed Biocompatible Micro-Bellows Membranes

Moussi, Khalil; Kosel, Jürgen

doi:10.1109/jmems.2018.2819994

Cited by 13 publications

(12 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The 3D printed pyramidal MNs were prepared using a direct laser writing system (Nanoscribe, GmbH, Photonics Professional GT) [21], utilizing two-photon polymerization of photoresist (IP-L780, Nanoscribe, GmbH) that was drop cast on a borosilicate glass coverslip (area of 2.5 cm 2 and thickness of 180 µm) ( Fig. 2 (a-b)).…”

Section: A Fabricationmentioning

confidence: 99%

3D Printed Microneedle Array for Electroporation

Moussi

Kavaldzhiev

Perez

et al. 2020

2020 42nd Annual International Conference of the IEEE Engineering in Medicine &Amp; Biology Society (EMBC)

Self Cite

View full text Add to dashboard Cite

In-vitro transfection of cells by electroporation is a widely used approach in cell biology and medicine. The transfection method is highly dependent on the cell culture's electrical resistance, which is strongly determined by differences in the membranes, but also on the morphology of the electrodes. Microneedle (MN)-based electrodes have been used to concentrate the electrical field during electroporation, and therefore maximize its effect on cell membrane permeability. So far, the methods used for the fabrication of MN electrodes have been relatively limited with respect to the needle design. In this work, we provide a method to fabricate MNs using 3D printing, which is a technology that provides a high degree of flexibility with respect to geometry and dimensions. Pyramidal-shaped MN designs were fabricated and tested on HCT116 cancer cells. Customization of the tips of the pyramids permits tailoring of the electrical field in the vicinity of the cell membranes. The fabricated device enables low-voltage (2 V) electroporation, eliminating the need for the use of specialized chemical buffers. The results show the potential of this method, which can be exploited and optimized for many different applications, and offer a very accessible approach for in-vitro electroporation and cell studies. The MNs can be customized to create complex structures, for example, for a multi-culture cell environment.

show abstract

Section: A Fabricationmentioning

confidence: 99%

3D Printed Microneedle Array for Electroporation

Moussi

Kavaldzhiev

Perez

et al. 2020

2020 42nd Annual International Conference of the IEEE Engineering in Medicine &Amp; Biology Society (EMBC)

Self Cite

View full text Add to dashboard Cite

show abstract

“…The fabrication process of the Parylene C micro-bellows membrane summarized in Fig. 3 was previously reported [9]. The membrane's dimensions were 3 mm by 1.2 mm in length and width, respectively, with a height varying from 0.5 mm, when the membrane is fully folded (deflated), to about 2 mm, when it is fully expended (inflated).…”

Section: A Micropump Fabricationmentioning

confidence: 99%

“…Bellow membranes are suitable as actuators for microelectrolytic pumps, due to their fitting pressure ranges [9]. The predictable performance and expansion profile of microbellows translates to controlled dosage in drug delivery.…”

Section: Introductionmentioning

confidence: 99%

“…In this study, a miniaturized and wirelessly powered drug delivery system is presented. The system includes an inductive powering unit, interdigitated electrodes for electrolysis, a Parylene C micro-bellows membrane [9], and a 3D printed reservoir with microneedles. Utilizing the tools and components mentioned, the presented design displays some advantages in size, powering, integration ease, versatility, and flexibility.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Miniaturized Drug Delivery System for Biomedical Applications

Moussi

AlDajani

Kosel

2019

2019 IEEE 14th International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)

Self Cite

View full text Add to dashboard Cite

A miniaturized 3D printed drug delivery device powered wirelessly is presented. The device is composed of an electrochemical micropump, a 3D printed reservoir with microneedles, and a wireless powering unit. The electrochemical pump features an expandable (up to 300%) Parylene C microbellows membrane fabricated by mold casting, using a twophoton polymerization 3D printing technique. The hollow microneedles are 100 µm in diameter and 300 µm long. The fabrication process offers customizable device properties, where the shape, size, and performance can be tailored to fit a wide range of in vivo drug delivery applications. Delivery of 3.8 ± 0.3 µL within 10 seconds of actuation is demonstrated, using inductive wireless powering at a distance of 10 mm between the primary and secondary coils.

show abstract

“…Thus, with the advent of TPP, it is now feasible to 3D print microstructures having dimensions in the millimeter scale while providing feature sizes with micrometer resolution. [35,36] Hence, in this work, the TPP 3D printing technique is used to fabricate various designs of hollow MNs, made of IP-S photoresist, with an aspect ratio (height/outer diameter of the MN tip) ranging from 1 to 20. The design, fabrication, fluidic, and mechanical properties are studied, and the biocompatibility is investigated.…”

Section: Introductionmentioning

confidence: 99%

Biocompatible 3D Printed Microneedles for Transdermal, Intradermal, and Percutaneous Applications

et al. 2019

Self Cite

View full text Add to dashboard Cite

Microneedles (MNs) are playing an increasingly important role in biomedical applications, where minimally invasive methods are being developed that require imperceptible tissue penetration and drug delivery. To improve the integration of MNs in microelectromechanical devices, a high‐resolution 3D printing technique is implemented. A reservoir with an array of hollow MNs is produced. The flow rate through the MNs is simulated and measured experimentally. The mechanical properties of the 3D printed material, such as elasticity modulus and yield strength, are investigated as functions of printing parameters, reaching maximum values of 1750.7 and 101.8 MPa, respectively. Analytical estimation of the MN buckling, fracture, and skin penetration forces is presented. Penetration tests of MNs into a skin‐like material are conducted, where the piercing force ranges from 0.095 to 0.115 N, confirming sufficient stability of MNs. Furthermore, 200 and 400 μm‐long MN arrays are used to successfully pierce and deliver into mouse skin with an average penetration depth of 100 and 180 μm, respectively. A biocompatibility assessment is performed, showing a high viability of HCT 116 cells cultured on top of the MN's material, making the developed MNs a very attractive solution for many biomedical applications.

show abstract

3-D Printed Biocompatible Micro-Bellows Membranes

Cited by 13 publications

References 23 publications

3D Printed Microneedle Array for Electroporation

3D Printed Microneedle Array for Electroporation

Miniaturized Drug Delivery System for Biomedical Applications

Biocompatible 3D Printed Microneedles for Transdermal, Intradermal, and Percutaneous Applications

Contact Info

Product

Resources

About