3-D printed microfluidics for rapid prototyping and testing of electrochemical, aptamer-based sensor devices under flow conditions

Belmonte, Israel; White, Raymond P.

doi:10.1016/j.aca.2021.339377

Cited by 10 publications

(10 citation statements)

References 36 publications

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“…On the other hand, sensors fabricated on dendritic modified electrode surfaces (2 mM HAuCl 4 modified) exhibit a 45% signal change upon 1 mM target addition compared to 120% reported in previous work done by our group. 35 Of note, we also explored 4 mM, 6 mM, and 8 mM HAuCl 4 surface modification and fabricated sensors on these platforms but observed no appreciable signal change (<60%) upon target interrogation (results not shown). This difference in percent signal change is a result of aptamer aggregation on the "flaky" modified electrode surface.…”

Section: ■ Results and Discussionmentioning

confidence: 93%

“…Even though aptamer probe packing densities are relatively the same for both surface modification, we hypothesized that sensors fabricated on a 2 mM modified surface have limited aptamer probe spacing which imposes restriction on target binding, thus affecting redox probe signal transduction. On the other hand, sensors fabricated on dendritic modified electrode surfaces (2 mM HAuCl 4 modified) exhibit a 45% signal change upon 1 mM target addition compared to 120% reported in previous work done by our group . Of note, we also explored 4 mM, 6 mM, and 8 mM HAuCl 4 surface modification and fabricated sensors on these platforms but observed no appreciable signal change (<60%) upon target interrogation (results not shown).…”

Section: Resultsmentioning

confidence: 98%

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Microscale, Electrochemical, Aptamer-Based Sensors for Enhanced Small-Molecule Detection at Millisecond Time Scales

Kumakli,

Baldwin,

Abeykoon

et al. 2023

ACS Sens.

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Section: ■ Results and Discussionmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 98%

Microscale, Electrochemical, Aptamer-Based Sensors for Enhanced Small-Molecule Detection at Millisecond Time Scales

Kumakli,

Baldwin,

Abeykoon

et al. 2023

ACS Sens.

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“…In contrast, the 3′ end is modified with a redox marker like methylene blue . In the presence of the target, the aptamer undergoes a conformational change bringing the redox tag closer to the electrode surface, facilitating the electron transfer process . SWV is particularly well-suited to monitor this class of sensor because of the ability to reduce background currents (non-faradaic charging current) while maximizing signaling differences between the target-free and target-bound states.…”

Section: Resultsmentioning

confidence: 99%

“…20 In the presence of the target, the aptamer undergoes a conformational change bringing the redox tag closer to the electrode surface, facilitating the electron transfer process. 21 SWV is particularly well-suited to monitor this class of sensor because of the ability to reduce background currents (non-faradaic charging current) while maximizing signaling differences between the target-free and target-bound states. White and Plaxco reported the optimization of electrochemical signaling via the variation of interrogation frequency as well as a measure of the apparent charge transfer rates with these types of folding-based sensors.…”

Section: Cswv To Interrogate Electrochemical Aptamer-based (E-ab) Sen...mentioning

confidence: 99%

Continuous Square Wave Voltammetry for High Information Content Interrogation of Conformation Switching Sensors

Abeykoon

White

2022

ACS Meas. Sci. Au

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Square wave voltammetry (SWV) is a voltammetric technique for measuring Faradaic current while minimizing contributions from non-Faradaic processes. In square wave voltammetry, the potential waveform applied to a working electrode and the current sampling protocols followed are designed to minimize contributions from non-Faradaic processes (i.e., double layer charging) to improve voltammetric sensitivity. To achieve this, the current is measured at the end of each forward and reverse potential pulse after allowing time for non-Faradaic currents to decay exponentially. A consequence of sampling current at the end of a potential pulse is that the current data from the preceding time of the potential pulse are discarded. These discarded data can provide information about the non-Faradaic contributions as well as information about the redox system including charge transfer rates. In this paper, we introduce continuous square wave voltammetry (cSWV), which utilizes the continuous collection of current to maximize the information content obtainable from a single voltammetry sweep eliminating the need for multiple scans. cSWV enables acquiring a multitude of voltammograms corresponding to various frequencies and, thus, different scan rates from a single sweep. An application that benefits significantly from cSWV is conformation switching, functional nucleic acid sensors. We demonstrate the utility of cSWV on two representative small molecules targeting electrochemical, aptamer-based sensors. Moreover, we show that cSWV provides comparable results to those obtained from traditional square wave voltammetry, but with cSWV, we are able to acquire dynamic information about the sensor surfaces enabling rapid calibration and optimization of sensing performance. We also demonstrate cSWV on soluble redox markers. cSWV can potentially become a mainstay technique in the field of conformation switching sensors.

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“…The small size of the voltammetric biosensor, the simplicity of the voltammetric analysis method and the flexibility of the chemometric method are ideal to obtain the target result from the information obtained by the voltammetric biosensor, for the simple operation and quick analysis of the results in various fields. POCT provides the premise [ 99 ]. In particular, devices with excellent computing power and network connectivity, represented by smartphones, provide a good combination for the application of voltammetric sensor systems assisted by chemometric technology for on-site detection and analysis.…”

Section: Applications Of Voltammetric Biosensing In Poctmentioning

confidence: 99%

Application and Progress of Chemometrics in Voltammetric Biosensing

Liu

et al. 2022

Biosensors

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The voltammetric electrochemical sensing method combined with biosensors and multi-sensor systems can quickly, accurately, and reliably analyze the concentration of the main analyte and the overall characteristics of complex samples. Simultaneously, the high-dimensional voltammogram contains the rich electrochemical features of the detected substances. Chemometric methods are important tools for mining valuable information from voltammetric data. Chemometrics can aid voltammetric biosensor calibration and multi-element detection in complex matrix conditions. This review introduces the voltammetric analysis techniques commonly used in the research of voltammetric biosensor and electronic tongues. Then, the research on optimizing voltammetric biosensor results using classical chemometrics is summarized. At the same time, the incorporation of machine learning and deep learning has brought new opportunities to further improve the detection performance of biosensors in complex samples. Finally, smartphones connected with miniaturized voltammetric biosensors and chemometric methods provide a high-quality portable analysis platform that shows great potential in point-of-care testing.

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3-D printed microfluidics for rapid prototyping and testing of electrochemical, aptamer-based sensor devices under flow conditions

Cited by 10 publications

References 36 publications

Microscale, Electrochemical, Aptamer-Based Sensors for Enhanced Small-Molecule Detection at Millisecond Time Scales

Microscale, Electrochemical, Aptamer-Based Sensors for Enhanced Small-Molecule Detection at Millisecond Time Scales

Continuous Square Wave Voltammetry for High Information Content Interrogation of Conformation Switching Sensors

Application and Progress of Chemometrics in Voltammetric Biosensing

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