3-Hydroxybenzoic Acid as an Internal Standard for the High-Pressure Liquid Chromatography Quantitation of Salicylic Acid in Plants

A rapid biosensor-based method for quantification of free and glucose-conjugated salicylic acid

“…Additionally, little luminescence was induced by pathogen-treated sid2 extracts, suggesting that if there is a compound other than SA that induces ADPWH_ lux , it is not present in sid2 , and may be derived from isochorismate. Another possibility is that recovery of SA using HPLC- and GC/MS-based methods which include organic solvent extraction and evaporation steps result in partial recovery of SA [ 38 ], despite inclusion of internal standards to account for the loss of SA. Although these internal standards have been shown to have similar recovery rates to SA [ 38 ], a difference in SA recovery between methods cannot be ruled out.…”

“…Another possibility is that recovery of SA using HPLC- and GC/MS-based methods which include organic solvent extraction and evaporation steps result in partial recovery of SA [ 38 ], despite inclusion of internal standards to account for the loss of SA. Although these internal standards have been shown to have similar recovery rates to SA [ 38 ], a difference in SA recovery between methods cannot be ruled out. Additionally, differences in photoperiod, pathogen inoculum, and the time after inoculation when SA content is measured, may also contribute to differences in SA measurements across different studies.…”

Combining bar adsorptive microextraction with capillary electrophoresis—Application for the determination of phenolic acids in food matrices

“…For this purpose, 3-hydroxybenzoic acid was used as internal standard (IS) in order to correct the migration time and peak areas. The selection criteria for the IS included similar chemical structure with the target compounds, specific detection, endogenous absence in real samples, high detection sensitivity, and nontoxicity [26]. According to the literature [27,28], borate buffer was successfully applied for the separation of those four phenolic acids.…”

植物激素定量分析方法研究进展

“…(HPLC)、毛细管电泳(CE)、免疫法等分析方法具有 简单、方便和仪器价廉等特点. 在早期的研究中, 植 物激素的电化学分析方法主要是针对脱落酸 [63] 、赤 霉素 [64] 、玉米素和激动素 [65] [21,22,72,73] . [13,74] 和电子轰击离子源(EI) [16,75] [77] , 但 ESI 源具有更高的灵敏度和更 低的背景噪音 [78] .…”