3-Hydroxykynurenine as a Potential Ligand for Hsp70 Proteins and Its Effects on Drosophila Memory After Heat Shock

Zhuravlev, Aleksandr V.; Щеголев, Б. Ф.; Захаров, Г. А.; Ivanova, Polina N.; Никитина, Е. А.; Савватеева-Попова, Е. В.

doi:10.1007/s12035-021-02704-3

Cited by 2 publications

(3 citation statements)

References 61 publications

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“…4, a). The CS LI was still high after 3 h, indicating STM preservation, in agreement with (Zhuravlev et al, 2022). The strain with limk1 interference also preserved STM: although its LI (3 h) was only about 20 %, it did not statistically differ from that for CS, as well as from LI (0 h).…”

Section: H Stm Differs In Hybrids With and Without Limk1 Interferencesupporting

confidence: 75%

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LIM-kinase 1 effects on memory abilities and male courtship song in Drosophila depend on the neuronal type

Zhuravlev,

Zalomaeva,

Egozova

et al. 2023

Vestn. VOGiS

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The signal pathway of actin remodeling, including LIM-kinase 1 (LIMK1) and its substrate cofilin, regulates multiple processes in neurons of vertebrates and invertebrates. Drosophila melanogaster is widely used as a model object for studying mechanisms of memory formation, storage, retrieval and forgetting. Previously, active forgetting in Drosophila was investigated in the standard Pavlovian olfactory conditioning paradigm. The role of specific dopaminergic neurons (DAN) and components of the actin remodeling pathway in different forms of forgetting was shown. In our research, we investigated the role of LIMK1 in Drosophila memory and forgetting in the conditioned courtship suppression paradigm (CCSP). In the Drosophila brain, LIMK1 and p-cofilin levels appeared to be low in specific neuropil structures, including the mushroom body (MB) lobes and the central complex. At the same time, LIMK1 was observed in cell bodies, such as DAN clusters regulating memory formation in CCSP. We applied GAL4 × UAS binary system to induce limk1 RNA interference in different types of neurons. The hybrid strain with limk1 interference in MB lobes and glia showed an increase in 3-h short-term memory (STM), without significant effects on long-term memory. limk1 interference in cholinergic neurons (CHN) impaired STM, while its interference in DAN and serotoninergic neurons (SRN) also dramatically impaired the flies’ learning ability. By contrast, limk1 interference in fruitless neurons (FRN) resulted in increased 15–60 min STM, indicating a possible LIMK1 role in active forgetting. Males with limk1 interference in CHN and FRN also showed the opposite trends of courtship song parameters changes. Thus, LIMK1 effects on the Drosophila male memory and courtship song appeared to depend on the neuronal type or brain structure.

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Section: H Stm Differs In Hybrids With and Without Limk1 Interferencesupporting

confidence: 75%

“…Learning and courtship suppression tests in Drosophila males. Flies learning and STM were estimated in CCSP, as described in (Zhuravlev et al, 2022). In the case of long term memory (LTM), learning was performed by placing flies in food-containing glasses (20 mm diam., ~20 mm high) for 5 h (Kamyshev et al, 1999).…”

Section: Methodsmentioning

confidence: 99%

LIM-kinase 1 effects on memory abilities and male courtship song in Drosophila depend on the neuronal type

Zhuravlev,

Zalomaeva,

Egozova

et al. 2023

Vestn. VOGiS

Self Cite

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“…Heat shock induces apoptosis in young cd 1 flies [ 28 ]. In addition, it impairs STM in cd 1 [ 41 ]. In contrast, cn 1 shows the neuroprotective and anti-apoptotic activity [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

cd1 Mutation in Drosophila Affects Phenoxazinone Synthase Catalytic Site and Impairs Long-Term Memory

Zhuravlev

Ivanova

Makaveeva

et al. 2022

IJMS

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Being involved in development of Huntington’s, Parkinson’s and Alzheimer’s diseases, kynurenine pathway (KP) of tryptophan metabolism plays a significant role in modulation of neuropathology. Accumulation of a prooxidant 3-hydroxykynurenine (3-HOK) leads to oxidative stress and neuronal cell apoptosis. Drosophila mutant cardinal (cd1) with 3-HOK excess shows age-dependent neurodegeneration and short-term memory impairments, thereby presenting a model for senile dementia. Although cd gene for phenoxazinone synthase (PHS) catalyzing 3-HOK dimerization has been presumed to harbor the cd1 mutation, its molecular nature remained obscure. Using next generation sequencing, we have shown that the cd gene in cd1 carries a long deletion leading to PHS active site destruction. Contrary to the wild type Canton-S (CS), cd1 males showed defective long-term memory (LTM) in conditioned courtship suppression paradigm (CCSP) at days 5–29 after eclosion. The number of dopaminergic neurons (DAN) regulating fly locomotor activity showed an age-dependent tendency to decrease in cd1 relative to CS. Thus, in accordance with the concept “from the gene to behavior” proclaimed by S. Benzer, we have shown that the aberrant PHS sequence in cd1 provokes drastic LTM impairments and DAN alterations.

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3-Hydroxykynurenine as a Potential Ligand for Hsp70 Proteins and Its Effects on Drosophila Memory After Heat Shock

Cited by 2 publications

References 61 publications

LIM-kinase 1 effects on memory abilities and male courtship song in Drosophila depend on the neuronal type

LIM-kinase 1 effects on memory abilities and male courtship song in Drosophila depend on the neuronal type

cd1 Mutation in Drosophila Affects Phenoxazinone Synthase Catalytic Site and Impairs Long-Term Memory

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