3-O-Glucosylation of quercetin enhances inhibitory effects on the adipocyte differentiation and lipogenesis

Lee, Chang Won; Seo, Jae‐Hyun; Lee, Jisun; Choi, Ji Won; Cho, Sarang; Bae, Jae Youn; Sohng, Jae Kyung; Kim, Sung Oog; Kim, Jihoon; Park, Yong Il

doi:10.1016/j.biopha.2017.08.002

Cited by 42 publications

(29 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This compound has been reported to show anti-obesity, antidiabetic, antiatherosclerotic, and anti-inflammatory properties, being effects associated with its ability to increase the expression and membrane translocation of the glucose transporter Glut-4 , thus facilitating adipocyte glucose uptake; moreover, protocatechuic acid was able to upregulate the levels of adiponectin, Ppar-γ and Ampk , which can also account for the thus ameliorating of the insulin resistance and obesity-associated disorders [ 55 , 56 ]. When Filipina MAE is considered, quercetin-3-glucoside is one of the main analytes detected, with reported beneficial effects against experimental obesity, given its ability to improve the altered lipid profile and to ameliorate the higher weight gain in obese mice, as well as to modulate the expression of genes involved in lipid metabolism and adipocyte differentiation [ 57 , 58 ]. Moreover, fumaroprotocetraric acid isomer 2 was found in the two most active MAEs, Filipina and Italia ; this fatty acid possesses antioxidant and antimicrobial properties, which may be also involved in their beneficial effects observed [ 59 ].…”

Section: Resultsmentioning

confidence: 99%

Comparative Study of the Antioxidant and Anti-Inflammatory Effects of Leaf Extracts from Four Different Morus alba Genotypes in High Fat Diet-Induced Obesity in Mice

Leyva-Jiménez¹,

Ruiz-Malagón

Molina-Tijeras

et al. 2020

Antioxidants

View full text Add to dashboard Cite

Increased levels of reactive oxygen species (ROS) and a low-grade chronic inflammation in multiple organs have been demonstrated in obesity. Morus alba leaves extracts (MAEs) have been used in traditional medicine as anti-inflammatory agents. In this work, the bioactive compounds of different genotypes of M. alba L. (Filipina, Valenciana Temprana, Kokuso, and Italia) were analyzed not only by reverse phase high performance liquid chromatography–electrospray ionization-time of flight-mass spectrometry (RP-HPLC-ESI-TOF-MS) and hydrophilic interaction chromatography–electrospray ionization-time of flight-mass spectrometry (HILIC-ESI-TOF-MS), but also screened for in vitro and in vivo antioxidant activity by means of DPPH· radical scavenging assay and Caenorhabditis elegans model. These MAEs were administered daily in a model of diet-induced obesity in mice. Filipina and Italia genotypes significantly reduced weight gain, the glycemic levels in high fat diet, as well as, levels of LDL-cholesterol and triglycerides. Filipina and Italia MAEs also reduced the expression of proinflammatory mediators such as Tnf-α, Il-1β, Il-6 and increased the levels of adiponectin and AMPK, which exert anti-inflammatory effects. Moreover, Italia genotype ameliorated the intestinal barrier function. In conclusion, Filipina and Italia methanolic extracts show the highest antioxidant and anti-inflammatory effect, due to the presence of compounds such as protocatechuic acid or quercetin-3-glucoside, and they could be developed as a complementary treatment for obesity and metabolic disorders.

show abstract

Section: Resultsmentioning

confidence: 99%

Comparative Study of the Antioxidant and Anti-Inflammatory Effects of Leaf Extracts from Four Different Morus alba Genotypes in High Fat Diet-Induced Obesity in Mice

Leyva-Jiménez¹,

Ruiz-Malagón

Molina-Tijeras

et al. 2020

Antioxidants

View full text Add to dashboard Cite

show abstract

“…According to the HPLC analysis previously reported by our group, the concentration of genistein, daidzein, quercetin, and naringenin in LBE were determined as about 0.053 mg/g, 0.165 mg/g, 0.853 mg/g, and 0.08 mg/g, respectively [18]. These natural compounds showed antioxidant effects and exerted anti-diabetic and anti-lipogenic potentials in in vitro studies [21][22][23][24][25]. In the current study, a high dose of LBE supplementation reduced the levels of HbA1c along with RAGE, which was considered as an index of chronic hyperglycemic states [26], although a low dose of LBE treatment decreased only HbA1c, which is a more useful clinical biomarker of diabetes.…”

Section: Discussionmentioning

confidence: 73%

Effects of Lespedeza Bicolor Extract on Regulation of AMPK Associated Hepatic Lipid Metabolism in Type 2 Diabetic Mice

et al. 2019

View full text Add to dashboard Cite

Lespedeza bicolor (LB) is one of the ornamental plants used for the treatment of inflammation caused by oxidative damage. However, its beneficial effects on hyperglycemia-induced hepatic damage and the related molecular mechanisms remain unclear. We hypothesized that Lespedeza bicolor extract (LBE) would attenuate hyperglycemia-induced liver injury in type 2 diabetes mellitus (T2DM). Diabetes was induced by a low dosage of streptozotocin (STZ) injection (30 mg/kg) with a high fat diet in male C57BL/6J mice. LBE was administered orally at 100 mg/kg or 250 mg/kg for 12 weeks. LBE supplementation regardless of dosage ameliorated plasma levels of hemoglobin A1c (HbA1c) in diabetic mice. Moreover, both LBE supplementations upregulated AMP-activation kinase (AMPK), which may activate sirtuin1 (SIRT) associated pathway accompanied by decreased lipid synthesis at low dose of LBE supplementation. These changes were in part explained by reduced protein levels of oxidative stress (nuclear factor erythroid 2-related factor 2 (Nrf2) and catalase), inflammation (nuclear factor kappa B (NF-κB), interleukin-1β (IL-1β), interleukin-6 (IL-6), and nitric oxide synthases (iNOS)), and fibrosis (α-smooth muscle actin (α-SMA) and protein kinase C (PKC)) in diabetic liver. Taken together, LBE might be a potential nutraceutical to ameliorate hepatic damage by regulation of AMPK associated pathway via oxidative stress, inflammation, and fibrosis in T2DM.

show abstract

“…Flavonols such as quercetin, kaempferol, and its glycosides inhibited adipogenesis or promoted AMPK signaling in adipocytes [54,55]. In comparison of aglycone and its glycosides, quercetin glucoside showed strong activity in reduction of lipid accumulation and inhibited adipogenic factors such as C/EBP-β, -α, and aP2 than its aglycone, quercetin [54]. Wnt/β-catenin signaling is mandatory in adipogenesis, and it inhibited preadipocyte differentiation by regulation of β-catenin [25].…”

Section: Discussionmentioning

confidence: 99%

“…Flavonols including quercetin, kaempferol, and rutin are flavonoids with a ketone group in onions, tomatoes, apples, and berries [50]. Flavonols such as quercetin, kaempferol, and its glycosides inhibited adipogenesis or promoted AMPK signaling in adipocytes [54,55]. In comparison of aglycone and its glycosides, quercetin glucoside showed strong activity in reduction of lipid accumulation and inhibited adipogenic factors such as C/EBP-β, -α, and aP2 than its aglycone, quercetin [54].…”

Section: Discussionmentioning

confidence: 99%

“…Wnt/β-catenin signaling is mandatory in adipogenesis, and it inhibited preadipocyte differentiation by regulation of β-catenin [25]. Lee et al reported that IQ suppressed the adipogenesis in 3T3-L1 cells via the inhibition of Wnt/β-catenin signaling, regulation of lipid metabolism-related factors such as PPARγ, C/EBPα, SREBP-1, adiponectin, resistin, visfatin, and improvement of insulin resistance [54,56]. In addition, our results indicated the inhibitory effect of lipid accumulation of flavonoids from A. okamotoanum including QU, IQ, and AF, in particular IQ, by regulation of adipogenesis, lipolysis, and AMPK signaling.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Flavonoids from Acer okamotoanum Inhibit Adipocyte Differentiation and Promote Lipolysis in the 3T3-L1 Cells

2020

View full text Add to dashboard Cite

Flavonoids, quercitrin, isoquercitrin (IQ), and afzelin, were isolated from ethyl acetate fraction of Acer okamotoanum. We investigated anti-obesity effects and mechanisms of three flavonoids from A. okamotoanum in the differentiated 3T3-L1 cells. The differentiated 3T3-L1 cells increased triglyceride (TG) contents, compared with non-differentiated normal group. However, treatments of three flavonoids from A. okamotoanum decreased TG contents without cytotoxicity. In addition, they showed significant down-regulation of several adipogenic transcription factors, such as γ-cytidine-cytidine-adenosine-adenosine-thymidine/enhancer binding protein -α, -β, and peroxisome proliferator-activated receptor-γ, compared with non-treated control group. Furthermore, treatment of the flavonoids inhibited expressions of lipogenesis-related proteins including fatty acid synthase, adipocyte protein 2, and glucose transporter 4. Moreover, IQ-treated group showed significant up-regulation of lipolysis-related proteins such as adipose triglyceride lipase and hormone-sensitive lipase. In addition, flavonoids significantly activated 5 -adenosine monophosphate-activated protein kinase (AMPK) compared to control group. In particular, IQ showed higher inhibition of TG accumulation by regulation of pathways related with both adipogenesis and lipolysis, than other flavonoids. The present results indicated that three flavonoids of A. okamotoanum showed anti-obesity activity by regulation of adipocyte differentiation, lipolysis, and AMPK signaling, suggesting as an anti-obesity functional agents.Molecules 2020, 25, 1920 2 of 13 activation of hydrolyzing enzymes including hormone-sensitive lipase (HSL) and adipose triglyceride lipase (ATGL), resulting in the production of fatty acids and glycerol [9]. In addition, lipolysis is decreased by over-expression of adipogenic key transcription factors in the adipogenesis [9]. Moreover, phosphorylation of 5 -adenosine monophosphate-activated protein kinase (AMPK) is important for progression of obesity [10]. Therefore, many researchers are focused on treatment of obesity by regulation of AMPK pathway [10,11]. Recently, anti-obesity agents are popular and attracted much attention, but they have several side effects including diarrhea, headache, and gastrointestinal discomfort [12]. Therefore, finding of natural products without side effects for anti-obesity agents by inhibition of adipogenesis and promotion of lipolysis is one of strategies to prevent and treat obesity.Acer okamotoanum is widely distributed in Ulleung-do, Republic of Korea, as one variety of Korean endemic species [13]. A. okamotoanum has been reported to exert some biological activities such as anti-oxidant, anti-hypertension and immune improvement effect [14][15][16]. A. okamotoanum contains biological compounds such as cleomiscosin A and C, gallic acid, and β-amyrin [17]. We previously isolated flavonoids such as quercitrin (QU; quercetin-3-rhamoside), isoquercitrin (IQ; quercetin-3-glucoside), and afzelin (AF; kaempferol-3-rhamoside...

show abstract

3-O-Glucosylation of quercetin enhances inhibitory effects on the adipocyte differentiation and lipogenesis

Cited by 42 publications

References 38 publications

Comparative Study of the Antioxidant and Anti-Inflammatory Effects of Leaf Extracts from Four Different Morus alba Genotypes in High Fat Diet-Induced Obesity in Mice

Comparative Study of the Antioxidant and Anti-Inflammatory Effects of Leaf Extracts from Four Different Morus alba Genotypes in High Fat Diet-Induced Obesity in Mice

Effects of Lespedeza Bicolor Extract on Regulation of AMPK Associated Hepatic Lipid Metabolism in Type 2 Diabetic Mice

Flavonoids from Acer okamotoanum Inhibit Adipocyte Differentiation and Promote Lipolysis in the 3T3-L1 Cells

Contact Info

Product

Resources

About